Structured Review

FUJIFILM m tris hcl
M Tris Hcl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris hcl/product/FUJIFILM
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
m tris hcl - by Bioz Stars, 2020-09
92/100 stars

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Protease Inhibitor:

Article Title: Enhanced Epithelial-Mesenchymal Transition-like Phenotype in N-Acetylglucosaminyltransferase V Transgenic Mouse Skin Promotes Wound Healing *
Article Snippet: .. Cell samples were solubilized at 4 °C in TNE buffer consisting of 10 m m Tris-HCl (pH 7.8), 1% Nonidet P-40, 0.15 m NaCl, 1 m m EDTA, and protease inhibitor mixture (Wako, Osaka, Japan). .. For in vivo samples, skin specimens were crushed in liquid nitrogen and solubilized at 4 °C in TNE buffer.

Avidin-Biotin Assay:

Article Title: Regulation of Burst Activity through Presynaptic and Postsynaptic GABAB Receptors in Mouse Superior Colliculus
Article Snippet: .. After washes in PBS, the sections were incubated with biotinylated goat anti-rabbit IgG antibody at room temperature for 2 h, washed, and reacted with avidin–biotin peroxidase complex (ABC elite kit; Vector Laboratories) at room temperature for 2 h. Subsequently, the sections were incubated in 50 m m Tris-HCl, pH 7.3, containing 0.025% 3–3-diaminobenzidine tetrahydrochloride (DAB; Wako, Osaka, Japan) and 0.003% hydrogen peroxide. .. Specificity of the immunolabeling was demonstrated by the absence of labeling for GABAB R1a/b when the primary antibody was omitted.

Purification:

Article Title: Crystal structures of the archaeal RNase P protein Rpp38 in complex with RNA fragments containing a K-turn motif
Article Snippet: .. The purified P10/12 was incubated with Pho Rpp21 and Pho Rpp29 in the presence of His-tagged Pho Rpp38 in a buffer consisting of 50 m M Tris–HCl pH 8.0 containing 5 m M MgCl2 and 500 m M NaCl at 50°C for 10 min, and the complexes were applied onto an Ni–NTA column (Wako Pure Chemical Industries, Osaka, Japan). .. After incubation for 30 min at room temperature and extensive washing, the complexes bound to the column were eluted with a buffer containing 0.5 M imidazole and the effluent was subjected to SDS–PAGE using the WIDE RANGE Gel Preparation buffer and visualized by staining the gels with Coomassie Brilliant Blue solution.

Activation Assay:

Article Title: Intermolecular autocatalytic activation of serine protease zymogen factor C through an active transition state responding to lipopolysaccharide
Article Snippet: .. For autocatalytic activation, factor C recombinants were incubated with 0.68 μ m (1.7 μg/ml) LPS at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl and subjected to Western blotting with anti-B chain antibody or anti-PA antibody (Wako, Osaka, Japan). .. In the case of amidase activity against Boc–Val–Pro–Arg– p NA, factor C recombinants were incubated with 0.68 μ m LPS for 30 min at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl.

Incubation:

Article Title: Crystal structures of the archaeal RNase P protein Rpp38 in complex with RNA fragments containing a K-turn motif
Article Snippet: .. The purified P10/12 was incubated with Pho Rpp21 and Pho Rpp29 in the presence of His-tagged Pho Rpp38 in a buffer consisting of 50 m M Tris–HCl pH 8.0 containing 5 m M MgCl2 and 500 m M NaCl at 50°C for 10 min, and the complexes were applied onto an Ni–NTA column (Wako Pure Chemical Industries, Osaka, Japan). .. After incubation for 30 min at room temperature and extensive washing, the complexes bound to the column were eluted with a buffer containing 0.5 M imidazole and the effluent was subjected to SDS–PAGE using the WIDE RANGE Gel Preparation buffer and visualized by staining the gels with Coomassie Brilliant Blue solution.

Article Title: Intermolecular autocatalytic activation of serine protease zymogen factor C through an active transition state responding to lipopolysaccharide
Article Snippet: .. For autocatalytic activation, factor C recombinants were incubated with 0.68 μ m (1.7 μg/ml) LPS at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl and subjected to Western blotting with anti-B chain antibody or anti-PA antibody (Wako, Osaka, Japan). .. In the case of amidase activity against Boc–Val–Pro–Arg– p NA, factor C recombinants were incubated with 0.68 μ m LPS for 30 min at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl.

Article Title: Regulation of Burst Activity through Presynaptic and Postsynaptic GABAB Receptors in Mouse Superior Colliculus
Article Snippet: .. After washes in PBS, the sections were incubated with biotinylated goat anti-rabbit IgG antibody at room temperature for 2 h, washed, and reacted with avidin–biotin peroxidase complex (ABC elite kit; Vector Laboratories) at room temperature for 2 h. Subsequently, the sections were incubated in 50 m m Tris-HCl, pH 7.3, containing 0.025% 3–3-diaminobenzidine tetrahydrochloride (DAB; Wako, Osaka, Japan) and 0.003% hydrogen peroxide. .. Specificity of the immunolabeling was demonstrated by the absence of labeling for GABAB R1a/b when the primary antibody was omitted.

Lysis:

Article Title: Testosterone induces activation of porcine primordial follicles in vitro
Article Snippet: .. Fresh ovarian cortical strips (1 mm × 1 mm × 1 mm) containing primordial follicles (15–20 strips, weighing 20 mg), were collected and washed 3 times in PBS and homogenized by means of a glass homogenizer kept on ice in a radioimmune precipitation assay (RIPA) lysis buffer containing 50 m m Tris–HCl (pH 7.4), 150 m m NaCl, 1 m m EDTA, 0.1% ( w / v ) sodium dodecyl sulfate (SDS), 0.5 m m p ‐aminophenyl methane sulfonyl fluoride hydrochloride ( p ‐APMSF; Wako Pure Chemical Industries), and 1% ( v / v ) Triton X‐100. .. The lysates were transferred to an Eppendorf tube to be sonicated by Vibra cell™ (Sonics and Materials, Danbury, CT, USA) with three short bursts of 10 s at 20% amplitude followed by intervals of 1 min on ice.

Western Blot:

Article Title: Intermolecular autocatalytic activation of serine protease zymogen factor C through an active transition state responding to lipopolysaccharide
Article Snippet: .. For autocatalytic activation, factor C recombinants were incubated with 0.68 μ m (1.7 μg/ml) LPS at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl and subjected to Western blotting with anti-B chain antibody or anti-PA antibody (Wako, Osaka, Japan). .. In the case of amidase activity against Boc–Val–Pro–Arg– p NA, factor C recombinants were incubated with 0.68 μ m LPS for 30 min at 37 °C in 20 m m Tris-HCl (pH 8.0) containing 300 m m NaCl.

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    FUJIFILM chemiluminescence ecl detection solution detection solution solution a 1 2 mm luminol in 0 1 m tris hcl
    Chemiluminescence Ecl Detection Solution Detection Solution Solution A 1 2 Mm Luminol In 0 1 M Tris Hcl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    FUJIFILM m ice cold tris hcl
    M Ice Cold Tris Hcl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    FUJIFILM m tris hcl
    M Tris Hcl, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/FUJIFILM
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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