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Bio-Rad m tris hcl
M Tris Hcl, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris hcl/product/Bio-Rad
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m tris hcl - by Bioz Stars, 2021-03
86/100 stars

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SDS Page:

Article Title: Identification of Potential Glycoprotein Biomarkers in Estrogen Receptor Positive (ER+) and Negative (ER-) Human Breast Cancer Tissues by LC-LTQ/FT-ICR Mass Spectrometry
Article Snippet: By use of PROTEAN IEF Cell (Bio-Rad), isoelectric focusing was performed according to the following procedure: rehydration of the IPG strips was done at 20 ˚C and 50 V for 16 h followed by focusing the proteins at 250 V for 15 min and then at 8,000 V maintained for a total of 50,000 V h per gel. .. 10% SDS-PAGE Tris-HCl Criterion (Bio-Rad, Hercules, CA) gels were prepared for 2-DE. ..

Article Title: Deletion of the Cel48S cellulase from Clostridium thermocellum
Article Snippet: The slope of these measurements was used to determine the initial hydrolysis rate (μmol/min) for a range of different cellulosome loadings from 0.05 to 0.8 mg. One unit (U) of enzyme activity releases 1 μmol SGE/min. .. Ten-microliter aliquots of the fermentation supernatant and 1.25-μL aliquots of purified cellulosome were run on a 7.5% Tris-HCl SDS/PAGE gel (Bio-Rad) to visualize the individual subunits ( ). .. Bands from the three purified lanes were excised, and the major components were analyzed by mass spectrometry (NextGen Science) ( ).

Article Title: Expression and Localization of an Hsp70 Protein in the Microsporidian Encephalitozoon cuniculi
Article Snippet: The strips were focused using a Biorad Protean IEF cell and the standard recommendations for programming. .. For the second dimension, the gel strips were equilibrated in the kit Equilibration Buffer I and II and precast Criterion 8–16% Tris-HCl SDS-PAGE gels (Biorad) were used. ..

Article Title: Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation
Article Snippet: Afterwards, mitochondrial, cytoplasmic and nuclear fractions were separated by differential centrifugation. .. All isolated fractions were separated in 4–15% Criterion Tris-HCl precast SDS-PAGE gels (Bio-Rad) and tested by a standard western blotting procedure. .. Each fraction was tested with the following primary antibodies: histone H3 (Sigma, dilution 1:200), VDAC (Mitosciences MSA03, dilution 1:1000), tubulin (Sigma, dilution 1:1000) and a polyclonal rabbit antiserum raised against DmLRPPRC2 (generated by PSL GmbH, dilution 1:1000).

Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation
Article Snippet: SH3-SH2 was purified through HiTrap™ SP HP column (GE Healthcare) in 20 mM MOPS pH 7.0 with a linear gradient of NaCl from 0 to 50% in 52 min. .. Abl kinase samples at different purification stages were subjected to 12% Tris-HCl SDS-PAGE gels (Bio-Rad), then transferred to PVDF membranes (Bio-Rad) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). ..

Article Title: Identification of Conus
Article Snippet: Following IEF, strips were reduced in equilibration buffer (75 m m Tris-HCl, 6 m urea, 30% glycerol, 2% SDS, 0.002% bromphenol blue) containing 65 m m dithiothreitol for 15 min followed by alkylation for 15 min in the presence of 80 m m iodoacetamide. .. Second dimension gel electrophoreses were performed on 8–16% Tris-HCl SDS-PAGE (Criterion, Bio-Rad) for 50 min at 200 V. Gels were stained with Coomassie Brilliant Blue G-250 (Bio-Rad). ..

Purification:

Article Title: Deletion of the Cel48S cellulase from Clostridium thermocellum
Article Snippet: The slope of these measurements was used to determine the initial hydrolysis rate (μmol/min) for a range of different cellulosome loadings from 0.05 to 0.8 mg. One unit (U) of enzyme activity releases 1 μmol SGE/min. .. Ten-microliter aliquots of the fermentation supernatant and 1.25-μL aliquots of purified cellulosome were run on a 7.5% Tris-HCl SDS/PAGE gel (Bio-Rad) to visualize the individual subunits ( ). .. Bands from the three purified lanes were excised, and the major components were analyzed by mass spectrometry (NextGen Science) ( ).

Article Title: Abl Kinase Constructs Expressed in Bacteria: facilitation of structural and functional studies including segmental labeling by expressed protein ligation
Article Snippet: SH3-SH2 was purified through HiTrap™ SP HP column (GE Healthcare) in 20 mM MOPS pH 7.0 with a linear gradient of NaCl from 0 to 50% in 52 min. .. Abl kinase samples at different purification stages were subjected to 12% Tris-HCl SDS-PAGE gels (Bio-Rad), then transferred to PVDF membranes (Bio-Rad) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). ..

Isolation:

Article Title: Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation
Article Snippet: Afterwards, mitochondrial, cytoplasmic and nuclear fractions were separated by differential centrifugation. .. All isolated fractions were separated in 4–15% Criterion Tris-HCl precast SDS-PAGE gels (Bio-Rad) and tested by a standard western blotting procedure. .. Each fraction was tested with the following primary antibodies: histone H3 (Sigma, dilution 1:200), VDAC (Mitosciences MSA03, dilution 1:1000), tubulin (Sigma, dilution 1:1000) and a polyclonal rabbit antiserum raised against DmLRPPRC2 (generated by PSL GmbH, dilution 1:1000).

Western Blot:

Article Title: Drosophila melanogaster LRPPRC2 is involved in coordination of mitochondrial translation
Article Snippet: Afterwards, mitochondrial, cytoplasmic and nuclear fractions were separated by differential centrifugation. .. All isolated fractions were separated in 4–15% Criterion Tris-HCl precast SDS-PAGE gels (Bio-Rad) and tested by a standard western blotting procedure. .. Each fraction was tested with the following primary antibodies: histone H3 (Sigma, dilution 1:200), VDAC (Mitosciences MSA03, dilution 1:1000), tubulin (Sigma, dilution 1:1000) and a polyclonal rabbit antiserum raised against DmLRPPRC2 (generated by PSL GmbH, dilution 1:1000).

Electrophoresis:

Article Title: Conditional Disruption of the Peroxisome Proliferator-Activated Receptor ? Gene in Mice Results in Lowered Expression of ABCA1, ABCG1, and apoE in Macrophages and Reduced Cholesterol Efflux
Article Snippet: Protein contained in the nuclear extracts of macrophages or total protein of Hepa-1 cells was assayed by the BCA protein assay (Pierce Chemical Co., Rockford, Ill.). .. Then, 10 μg of total protein from Hepa-1 cells and 10 μg of protein from nuclear extracts of macrophages were subjected to electrophoresis on a 4 to 15% Tris-HCl gradient gel (Bio-Rad, Hercules, Calif.), transferred to Immobilon-P membranes (Millipore, Bedford, Mass.), and probed according to the manufacturer's recommendations with anti-PPARγ antibodies (E-8 and H-100; Santa Cruz Biotechnology, Inc., Santa Cruz, Calif.) as indicated. .. Detection of immunoreactive proteins was done by an enhanced chemiluminescence blot detection system (Amersham, Inc., Arlington Heights, Ill.).

Staining:

Article Title: Identification of Conus
Article Snippet: Following IEF, strips were reduced in equilibration buffer (75 m m Tris-HCl, 6 m urea, 30% glycerol, 2% SDS, 0.002% bromphenol blue) containing 65 m m dithiothreitol for 15 min followed by alkylation for 15 min in the presence of 80 m m iodoacetamide. .. Second dimension gel electrophoreses were performed on 8–16% Tris-HCl SDS-PAGE (Criterion, Bio-Rad) for 50 min at 200 V. Gels were stained with Coomassie Brilliant Blue G-250 (Bio-Rad). ..

Article Title: The XcpV/GspI Pseudopilin Has a Central Role in the Assembly of a Quaternary Complex within the T2SS Pseudopilus *
Article Snippet: The digitalized gels were then analyzed with the free software “ImageJ for Mac” ( ) to quantify and compare the intensity of the protein bands. .. The eluted complex presented in C ( panel 1 , lane E ) has been electrophoresed on a Native 8–16% Tris-HCl PAGE (Bio-Rad) at 4 °C in a Tris/Glycine running buffer for 1 h at 50 V and a further 2 h at 125 V. The native-PAGE was then stained with Coomassie Brillant Blue R-250. ..

Polyacrylamide Gel Electrophoresis:

Article Title: The XcpV/GspI Pseudopilin Has a Central Role in the Assembly of a Quaternary Complex within the T2SS Pseudopilus *
Article Snippet: The digitalized gels were then analyzed with the free software “ImageJ for Mac” ( ) to quantify and compare the intensity of the protein bands. .. The eluted complex presented in C ( panel 1 , lane E ) has been electrophoresed on a Native 8–16% Tris-HCl PAGE (Bio-Rad) at 4 °C in a Tris/Glycine running buffer for 1 h at 50 V and a further 2 h at 125 V. The native-PAGE was then stained with Coomassie Brillant Blue R-250. ..

Clear Native PAGE:

Article Title: The XcpV/GspI Pseudopilin Has a Central Role in the Assembly of a Quaternary Complex within the T2SS Pseudopilus *
Article Snippet: The digitalized gels were then analyzed with the free software “ImageJ for Mac” ( ) to quantify and compare the intensity of the protein bands. .. The eluted complex presented in C ( panel 1 , lane E ) has been electrophoresed on a Native 8–16% Tris-HCl PAGE (Bio-Rad) at 4 °C in a Tris/Glycine running buffer for 1 h at 50 V and a further 2 h at 125 V. The native-PAGE was then stained with Coomassie Brillant Blue R-250. ..

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    Bio-Rad m tris hcl
    M Tris Hcl, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Bio-Rad
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2021-03
    86/100 stars
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