m tris carboxyethylphosphine  (Millipore)


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  • 99
    Name:
    Tris
    Description:

    Catalog Number:
    ge17-1321-01
    Price:
    None
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    Structured Review

    Millipore m tris carboxyethylphosphine
    Tris

    https://www.bioz.com/result/m tris carboxyethylphosphine/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris carboxyethylphosphine - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: S100P enhances the motility and invasion of human trophoblast cell lines
    Article Snippet: .. qPCR analysis Following the appropriate incubation, trophoblast cells were collected after trypsinisation and mRNA extraction was carried out using TRIS reagent (Sigma, UK) according to the manufacturer’s protocol. qPCR was performed using S100P (sequences F: 5′-TCAAGGTGCTGATGGAGAA-3′ and R: 5′-ACACGATGAACTCACTGAA-3′) and β-actin primers (F: 5′-ATGTACGTTGCTATCCAGGC-3′ and R: 5′-CTCCTTAATGTCACGCACGAT-3′) and SYBR green mix (Roche, UK) according to the manufacturer’s protocols. ..

    Flow Cytometry:

    Article Title: Bacterially Expressed F1-20/AP-3 Assembles Clathrin Into Cages With a Narrow Size Distribution: Implications for the Regulation of Quantal Size During Neurotransmission
    Article Snippet: .. The Sepharose was then eluted three times with 0.5 ml 0.5 M Tris, 0.1 mM PMSF (pH 7.0) at 37°C for 15 min. Flow-through, washes, and eluates were all concentrated using Millipore quick concentrator-10s, and each sample was brought to 20 µl in IX SDS sample buffer. ..

    Buffer Exchange:

    Article Title: Cj1386, an Atypical Hemin-Binding Protein, Mediates Hemin Trafficking to KatA in Campylobacter jejuni
    Article Snippet: .. Buffer exchange with 100 mM NaCl, 20 mM Tris, pH 7.4, was performed using a 10-kDa-cutoff centrifugal filter unit (Millipore). ..

    SYBR Green Assay:

    Article Title: S100P enhances the motility and invasion of human trophoblast cell lines
    Article Snippet: .. qPCR analysis Following the appropriate incubation, trophoblast cells were collected after trypsinisation and mRNA extraction was carried out using TRIS reagent (Sigma, UK) according to the manufacturer’s protocol. qPCR was performed using S100P (sequences F: 5′-TCAAGGTGCTGATGGAGAA-3′ and R: 5′-ACACGATGAACTCACTGAA-3′) and β-actin primers (F: 5′-ATGTACGTTGCTATCCAGGC-3′ and R: 5′-CTCCTTAATGTCACGCACGAT-3′) and SYBR green mix (Roche, UK) according to the manufacturer’s protocols. ..

    Incubation:

    Article Title: S100P enhances the motility and invasion of human trophoblast cell lines
    Article Snippet: .. qPCR analysis Following the appropriate incubation, trophoblast cells were collected after trypsinisation and mRNA extraction was carried out using TRIS reagent (Sigma, UK) according to the manufacturer’s protocol. qPCR was performed using S100P (sequences F: 5′-TCAAGGTGCTGATGGAGAA-3′ and R: 5′-ACACGATGAACTCACTGAA-3′) and β-actin primers (F: 5′-ATGTACGTTGCTATCCAGGC-3′ and R: 5′-CTCCTTAATGTCACGCACGAT-3′) and SYBR green mix (Roche, UK) according to the manufacturer’s protocols. ..

    other:

    Article Title: Quantum and electrochemical interplays in hydrogenated graphene
    Article Snippet: Electrolyte- or electrochemical-gated GFET measurements were carried out in 0.1 M KCl solution containing 10 mM Tris as the buffer (pH 8, both from Sigma Aldrich).

    Activity Assay:

    Article Title: Influence of sodium substitutes on 5-HT-mediated effects at mouse 5-HT3 receptors
    Article Snippet: .. The following compounds were used in the experiments (for chemical structures, see ): 5-hydroxytryptamine creatinine sulphate from Sigma, Munich, Germany; [14 C]guanidinium chloride (specific activity=59 mCi mmol−1 ) from CEA (Biotrend, Cologne, Germany); [3 H]GR65630, (specific activity 64.8 Ci mmol−1 ) from NEN DuPont (Dreieich, Germany); choline chloride, TMA chloride, Tris and NMDG were all from Sigma (Munich, Germany). .. Basal [ 14 C]guanidinium influx : In N1E-115 cells incubated in buffer containing 135 m M sodium, basal influx of [14 C]guanidinium, that is, influx in the absence of 5-HT, amounted to 16.0±0.5 pmol × mg protein−1 ( n =19).