m sodium pyrophosphate  (Roche)


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    Structured Review

    Roche m sodium pyrophosphate
    M Sodium Pyrophosphate, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m sodium pyrophosphate/product/Roche
    Average 91 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    m sodium pyrophosphate - by Bioz Stars, 2020-09
    91/100 stars

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    Related Articles

    Transfection:

    Article Title: The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis *
    Article Snippet: .. Cells were harvested 16–20 h after transfection and lysed in ice-cold buffer containing 10 m m HEPES, 130 m m NaCl, 1% Triton X-100, 10 m m NaF, 10 m m Na2 HPO4 , 10 m m sodium pyrophosphate, and protease inhibitor mixture (Roche Applied Science). ..

    Protease Inhibitor:

    Article Title: The Significance of the Bifunctional Kinase/Phosphatase Activities of Diphosphoinositol Pentakisphosphate Kinases (PPIP5Ks) for Coupling Inositol Pyrophosphate Cell Signaling to Cellular Phosphate Homeostasis *
    Article Snippet: .. Cells were harvested 16–20 h after transfection and lysed in ice-cold buffer containing 10 m m HEPES, 130 m m NaCl, 1% Triton X-100, 10 m m NaF, 10 m m Na2 HPO4 , 10 m m sodium pyrophosphate, and protease inhibitor mixture (Roche Applied Science). ..

    Article Title: Tti1 and Tel2 Are Critical Factors in Mammalian Target of Rapamycin Complex Assembly *
    Article Snippet: .. For immunoprecipitation analysis, cell lysates were prepared in a second lysis buffer (40 m m HEPES (pH 7.5), 150 m m NaCl, 2 m m EDTA, 0.3% CHAPS, 10 m m NaF, 0.4 m m Na3 VO4 , 10 m m sodium pyrophosphate, and Complete EDTA-free protease inhibitor (Roche Applied Science)). .. The lysates were clarified by centrifugation at 15,000 rpm for 15 min and then subjected to immunoprecipitation using specific antibodies in combination with protein A- or G-Sepharose (Amersham Biosciences).

    Article Title: Cytosolic Phospholipase A2 Activation Correlates with HER2 Overexpression and Mediates Estrogen-Dependent Breast Cancer Cell Growth
    Article Snippet: .. Cells were then transferred onto ice, washed twice with ice-cold PBS, and then ultrasonicated in lysis buffer [20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, 1 m m phenylmethylsulfonyl fluoride, complete mini EDTA-free protease inhibitor mixture tablets (one tablet per 10 ml lysis buffer; Roche) and phosphatase inhibitors]. .. Total protein concentration was quantified using the bicinchoninic acid assay ( ).

    Article Title: Type I Phosphotidylinosotol 4-Phosphate 5-Kinase ? Regulates Osteoclasts in a Bifunctional Manner *
    Article Snippet: .. Cultured cells were washed twice with ice-cold PBS and lysed in radioimmune precipitation assay buffer containing 20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 m m NaF, and 1× protease inhibitor mixture (Roche Applied Science). .. After incubation on ice for 10 min, cell lysates were clarified by centrifugation at 15,000 rpm for 10 min. Forty micrograms of total lysates were subjected to 8–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto PVDF membranes.

    Article Title: An activating mutation of interferon regulatory factor 4 (IRF4) in adult T-cell leukemia
    Article Snippet: .. Nuclear pellets were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science) and sonicated on ice for 20 s before use for analysis (modified from Ref. ). .. Cells were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science/Sigma).

    Cell Culture:

    Article Title: Type I Phosphotidylinosotol 4-Phosphate 5-Kinase ? Regulates Osteoclasts in a Bifunctional Manner *
    Article Snippet: .. Cultured cells were washed twice with ice-cold PBS and lysed in radioimmune precipitation assay buffer containing 20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 m m NaF, and 1× protease inhibitor mixture (Roche Applied Science). .. After incubation on ice for 10 min, cell lysates were clarified by centrifugation at 15,000 rpm for 10 min. Forty micrograms of total lysates were subjected to 8–12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto PVDF membranes.

    Immunoprecipitation:

    Article Title: Tti1 and Tel2 Are Critical Factors in Mammalian Target of Rapamycin Complex Assembly *
    Article Snippet: .. For immunoprecipitation analysis, cell lysates were prepared in a second lysis buffer (40 m m HEPES (pH 7.5), 150 m m NaCl, 2 m m EDTA, 0.3% CHAPS, 10 m m NaF, 0.4 m m Na3 VO4 , 10 m m sodium pyrophosphate, and Complete EDTA-free protease inhibitor (Roche Applied Science)). .. The lysates were clarified by centrifugation at 15,000 rpm for 15 min and then subjected to immunoprecipitation using specific antibodies in combination with protein A- or G-Sepharose (Amersham Biosciences).

    Infection:

    Article Title:
    Article Snippet: .. At the indicated times, infected HUVEC were washed once with PBS containing 1 m m MgCl2 and 1 m m CaCl2 and lysed in ice-cold modified radioimmune precipitation assay buffer (25 m m Hepes (pH 7.4), 1% Nonidet P-40, 0.25% sodium deoxycholate, 10% glycerol, 150 m m NaCl, 1 m m EDTA, 10 m m sodium pyrophosphate, 50 m m NaF, 2 m m NaVO3 , 2 m m phenylmethylsulfonyl fluoride, Complete MiniTM (Roche Applied Science). .. Equivalent amounts of the cleared lysates were added to an equal volume of reducing 2× SDS sample buffer, and the proteins were separated by SDS-PAGE.

    De-Phosphorylation Assay:

    Article Title: Cocaine Increases Actin Cycling: Effects in the Reinstatement Model of Drug Seeking
    Article Snippet: .. All buffers contained 1 n m microcystin LR (Sigma), 2 m m sodium pyrophosphate, 1 m m Na3 VO4 , 5 m m NaF, 10 m m sodium pyrophosphate, 20 m m β-glycerophosphatase, 0.1 m m ammonium molybdate, 1 m m para-nitrophenylphosphatase, 1 m m benzamide, and Complete Mini including EDTA (1 tablet/10 ml; Roche Products, Indianapolis, IN) to prevent degradation and dephosphorylation of proteins. ..

    Lysis:

    Article Title: Tti1 and Tel2 Are Critical Factors in Mammalian Target of Rapamycin Complex Assembly *
    Article Snippet: .. For immunoprecipitation analysis, cell lysates were prepared in a second lysis buffer (40 m m HEPES (pH 7.5), 150 m m NaCl, 2 m m EDTA, 0.3% CHAPS, 10 m m NaF, 0.4 m m Na3 VO4 , 10 m m sodium pyrophosphate, and Complete EDTA-free protease inhibitor (Roche Applied Science)). .. The lysates were clarified by centrifugation at 15,000 rpm for 15 min and then subjected to immunoprecipitation using specific antibodies in combination with protein A- or G-Sepharose (Amersham Biosciences).

    Article Title: Cytosolic Phospholipase A2 Activation Correlates with HER2 Overexpression and Mediates Estrogen-Dependent Breast Cancer Cell Growth
    Article Snippet: .. Cells were then transferred onto ice, washed twice with ice-cold PBS, and then ultrasonicated in lysis buffer [20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, 1 m m phenylmethylsulfonyl fluoride, complete mini EDTA-free protease inhibitor mixture tablets (one tablet per 10 ml lysis buffer; Roche) and phosphatase inhibitors]. .. Total protein concentration was quantified using the bicinchoninic acid assay ( ).

    Modification:

    Article Title:
    Article Snippet: .. At the indicated times, infected HUVEC were washed once with PBS containing 1 m m MgCl2 and 1 m m CaCl2 and lysed in ice-cold modified radioimmune precipitation assay buffer (25 m m Hepes (pH 7.4), 1% Nonidet P-40, 0.25% sodium deoxycholate, 10% glycerol, 150 m m NaCl, 1 m m EDTA, 10 m m sodium pyrophosphate, 50 m m NaF, 2 m m NaVO3 , 2 m m phenylmethylsulfonyl fluoride, Complete MiniTM (Roche Applied Science). .. Equivalent amounts of the cleared lysates were added to an equal volume of reducing 2× SDS sample buffer, and the proteins were separated by SDS-PAGE.

    Article Title: An activating mutation of interferon regulatory factor 4 (IRF4) in adult T-cell leukemia
    Article Snippet: .. Nuclear pellets were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science) and sonicated on ice for 20 s before use for analysis (modified from Ref. ). .. Cells were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science/Sigma).

    Sonication:

    Article Title: An activating mutation of interferon regulatory factor 4 (IRF4) in adult T-cell leukemia
    Article Snippet: .. Nuclear pellets were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science) and sonicated on ice for 20 s before use for analysis (modified from Ref. ). .. Cells were lysed in Tris-HCl, pH 6.8, with 2% Igepal CA630, 2% SDS, 10 m m sodium β-glycerophosphate, 10 m m sodium fluoride, 2.5 m m sodium pyrophosphate, 1 m m sodium orthovanadate, and EDTA-free protease inhibitor mixture (Roche Applied Science/Sigma).

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  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 91
    Roche m sodium pyrophosphate
    M Sodium Pyrophosphate, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m sodium pyrophosphate/product/Roche
    Average 91 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    m sodium pyrophosphate - by Bioz Stars, 2020-09
    91/100 stars
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