m sodium pyrophosphate  (Millipore)


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  • 96
    Name:
    Sodium pyrophosphate decahydrate
    Description:

    Catalog Number:
    28-3960
    Price:
    None
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    Structured Review

    Millipore m sodium pyrophosphate
    Sodium pyrophosphate decahydrate

    https://www.bioz.com/result/m sodium pyrophosphate/product/Millipore
    Average 96 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    m sodium pyrophosphate - by Bioz Stars, 2020-09
    96/100 stars

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    Related Articles

    Protease Inhibitor:

    Article Title: Importance of TFEB acetylation in control of its transcriptional activity and lysosomal function in response to histone deacetylase inhibitors
    Article Snippet: .. Briefly, the cells were lysed on ice for 30 min with the IP buffer (40 mM HEPES, pH 7.4, 120 mM NaCl, 2 mM EDTA, 0.3% CHAPS (Sigma, 10810118001), 10 mM pyrophosphate (Sigma, 221368), 10 mM glycerophosphate (Sigma, G9422), 50 mM NaF, phosphatase and protease inhibitor mixture). .. Cell lysates (1 μg protein) were incubated with anti-FLAG® M2 Affinity Gel or acetyl-lysine antibody conjugated agarose beads overnight with gentle rocking at 4°C.

    Article Title: USP12 translocation maintains interferon antiviral efficacy by inhibiting CBP acetyltransferase activity
    Article Snippet: .. Cytoplasmic and nuclear proteins extraction Cells were washed in cold PBS and then harvested in harvest lysis buffer containing 10 mM HEPES (PH 7.9), 50 mM NaCl, 0.5 mM Sucrose, 0.1 mM EDTA, 0.5% Triton X-100, 1mM DTT, 10 mM Sodium pyrophosphate decahydrate, 0.5 M NaF, 0.2 M Na3 VO4 , 1 mM PMSF and protease inhibitor mixtures (Sigma) on ice. ..

    Article Title: The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex
    Article Snippet: .. Cells were rinsed twice with ice-cold DPBS and lysed in ice-cold CHAPs lysis buffer [0.3% CHAPS (Sigma #C3023), 10 m m beta-glycerol phosphate (Sigma #G9422), 10 m m sodium pyrophosphate (Sigma #221368), 40 m m HEPES (pH 7.4) and 2.5 m m MgCl2 ] with one tablet of EDTA-free protease inhibitor (Roche #11836170001) per 10 ml as described ( ). .. Lysate were cleared by centrifugation at 16 000g at 4°C for 10 min. For anti-Flag IPs, cleared lysates were normalized for protein content by Bradford assay, and 2–3 mg of protein lysate were incubated with 40 ul of a 50% slurry of Flag-M2 affinity gel (Sigma #A2220) per 1 mg of lysate at 4°C rotating overnight.

    Centrifugation:

    Article Title: A negative feedback regulation of MTORC1 activity by the lysosomal Ca2+ channel MCOLN1 (mucolipin 1) using a CALM (calmodulin)-dependent mechanism
    Article Snippet: .. After centrifugation at 12,000 × g for 15 min to remove the beads, aliquots of cell lysates were incubated with the desired antibodies or control IgG at 4°C overnight in a final volume of 1 ml RIPA-PBS buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% NP-40 [Sigma-Aldrich, 74385], 1% sodium deoxycholate [Sigma-Aldrich, S1287], 2.5 mM sodium pyrophosphate [Sigma-Aldrich, 221368], 1 mM b-glycerophosphate [Cayman Chemical, 14405], 1 mM Na3 VO4 [Sigma-Aldrich, 450243], 1 µg/ml leupeptin [Sigma-Aldrich, L2884]) with constant rocking. .. After antibody incubation, protein A/G-agarose beads were added and the samples were incubated at 4°C for 4 h, followed by centrifugation at 300 g for 10 min at 4°C.

    Incubation:

    Article Title: A negative feedback regulation of MTORC1 activity by the lysosomal Ca2+ channel MCOLN1 (mucolipin 1) using a CALM (calmodulin)-dependent mechanism
    Article Snippet: .. After centrifugation at 12,000 × g for 15 min to remove the beads, aliquots of cell lysates were incubated with the desired antibodies or control IgG at 4°C overnight in a final volume of 1 ml RIPA-PBS buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 1 mM Na2 EDTA, 1 mM EGTA, 1% NP-40 [Sigma-Aldrich, 74385], 1% sodium deoxycholate [Sigma-Aldrich, S1287], 2.5 mM sodium pyrophosphate [Sigma-Aldrich, 221368], 1 mM b-glycerophosphate [Cayman Chemical, 14405], 1 mM Na3 VO4 [Sigma-Aldrich, 450243], 1 µg/ml leupeptin [Sigma-Aldrich, L2884]) with constant rocking. .. After antibody incubation, protein A/G-agarose beads were added and the samples were incubated at 4°C for 4 h, followed by centrifugation at 300 g for 10 min at 4°C.

    Lysis:

    Article Title: USP12 translocation maintains interferon antiviral efficacy by inhibiting CBP acetyltransferase activity
    Article Snippet: .. Cytoplasmic and nuclear proteins extraction Cells were washed in cold PBS and then harvested in harvest lysis buffer containing 10 mM HEPES (PH 7.9), 50 mM NaCl, 0.5 mM Sucrose, 0.1 mM EDTA, 0.5% Triton X-100, 1mM DTT, 10 mM Sodium pyrophosphate decahydrate, 0.5 M NaF, 0.2 M Na3 VO4 , 1 mM PMSF and protease inhibitor mixtures (Sigma) on ice. ..

    Article Title: The TMEM127 human tumor suppressor is a component of the mTORC1 lysosomal nutrient-sensing complex
    Article Snippet: .. Cells were rinsed twice with ice-cold DPBS and lysed in ice-cold CHAPs lysis buffer [0.3% CHAPS (Sigma #C3023), 10 m m beta-glycerol phosphate (Sigma #G9422), 10 m m sodium pyrophosphate (Sigma #221368), 40 m m HEPES (pH 7.4) and 2.5 m m MgCl2 ] with one tablet of EDTA-free protease inhibitor (Roche #11836170001) per 10 ml as described ( ). .. Lysate were cleared by centrifugation at 16 000g at 4°C for 10 min. For anti-Flag IPs, cleared lysates were normalized for protein content by Bradford assay, and 2–3 mg of protein lysate were incubated with 40 ul of a 50% slurry of Flag-M2 affinity gel (Sigma #A2220) per 1 mg of lysate at 4°C rotating overnight.

    Article Title: Safety and preliminary evidence of biological efficacy of a mammaglobin-A DNA vaccine in patients with stable metastatic breast cancer
    Article Snippet: .. Total proteins were extracted from cells with lysis buffer (50 mM HEPES [pH 7.6], 150 mM NaCl, 1% Triton X-100, 30 mM Na4 P2 O7 , 10% glycerol, 1 mM benzamidine, 1 mM DTT, 10 μg of leupeptin/mL, 1 mM phenylmethylsulfonyl fluoride, 50 mM NaF, 1 mM sodium orthovanadate, 10 mM sodium pyrophosphate decahydrate, and 10 mM β-glycerophosphate (Sigma Aldrich). .. After cell lysis, the supernatant was collected after centrifugation at 15,000 × g for 15 min at 4 °C ( ).

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  • 99
    Millipore sodium cacodylate buffer
    Sodium Cacodylate Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sodium cacodylate buffer/product/Millipore
    Average 99 stars, based on 173 article reviews
    Price from $9.99 to $1999.99
    sodium cacodylate buffer - by Bioz Stars, 2020-09
    99/100 stars
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