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Cell Signaling Technology Inc m sodium pyrophosphate
M Sodium Pyrophosphate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m sodium pyrophosphate/product/Cell Signaling Technology Inc
Average 91 stars, based on 7 article reviews
Price from $9.99 to $1999.99
m sodium pyrophosphate - by Bioz Stars, 2020-09
91/100 stars

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Lysis:

Article Title: Disruption of Nongenomic Testosterone Signaling in a Model of Spinal and Bulbar Muscular Atrophy
Article Snippet: .. Cell lysates were prepared in lysis buffer [20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m EGTA, 1 m m EDTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin; Cell Signaling] supplemented with protease inhibitors (Mini Complete; Roche, France). .. Lysates were centrifuged at 3000 × g for 10 min at 4 C, and protein concentrations of supernatants were determined by the BCA (bicinchoninic acid) method (Thermo Fisher Scientific, France).

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

Article Title: Proteomic Profiling of the Planarian Schmidtea mediterranea and Its Mucous Reveals Similarities with Human Secretions and Those Predicted for Parasitic Flatworms *
Article Snippet: .. Lysates were generated from whole organisms of the CIW4 clonal strain of asexual Schmidtea mediterranea , sized matched to 2–4 mm, using a tissue homogenizer and lysis buffer containing 20 m m HEPES buffer (Cell Signaling Technology, Boston, MA) 8 m urea (EMD Chemicals, Darmstadt, Germany), 1 m m sodium orthovanadate (BioShop Canada Inc., Burlington, ON, Canada), 2.5 m m sodium pyrophosphate (Cell Signaling Technology), and 1 m m β-glycerophosphate (Cell Signaling Technology). .. Whole organisms were also treated with 5% NAC (Sigma-Aldrich, St. Louis, MO) in 1× PBS (BioShop) for 8 min to remove their mucous coating , and subsequently lysed as described above.

Article Title: A Role for cGMP in Inducible Nitric-oxide Synthase (iNOS)-induced Tumor Necrosis Factor (TNF) ?-converting Enzyme (TACE/ADAM17) Activation, Translocation, and TNF Receptor 1 (TNFR1) Shedding in Hepatocytes *
Article Snippet: .. Treated hepatocytes were washed twice in PBS and lysed with 1× cell lysis buffer (Cell Signaling Technology) containing 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerol phosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, and 1 μg/ml phenylmethylsulfonyl fluoride (PMSF) on ice for 10 min. Liver samples were homogenized with a glass Dounce homogenizer in 1× radioimmune precipitation assay buffer (cell lysis buffer; Cell Signaling Technology) containing 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Nonidet P-40, 1% sodium deoxycholate, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 V04 , 1 μg/ml leupeptin, and 1 m m PMSF in double distilled H2 O. .. The protein content of cell lysates was determined by BCA protein assay (Pierce).

Article Title: NADPH:Quinone Oxidoreductase 1 Regulates Host Susceptibility to Ozone via Isoprostane Generation *
Article Snippet: .. Ozone-exposed cells were rinsed with ice-cold PBS containing 1 m m phenylmethylsulfonyl fluoride (PMSF) and lysed for 5 min on ice in Cell Lysis Buffer (20 m m Tris (pH 7.5), 150 m m NaCl, 1 m m EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m glycerol phosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, and 1 m m phenylmethylsulfonyl fluoride) (Cell Signaling Technology, Beverly, MA) containing both 1× Complete protease (Roche Applied Science) and 1× phosphatase inhibitor mixtures (Sigma). ..

Generated:

Article Title: Proteomic Profiling of the Planarian Schmidtea mediterranea and Its Mucous Reveals Similarities with Human Secretions and Those Predicted for Parasitic Flatworms *
Article Snippet: .. Lysates were generated from whole organisms of the CIW4 clonal strain of asexual Schmidtea mediterranea , sized matched to 2–4 mm, using a tissue homogenizer and lysis buffer containing 20 m m HEPES buffer (Cell Signaling Technology, Boston, MA) 8 m urea (EMD Chemicals, Darmstadt, Germany), 1 m m sodium orthovanadate (BioShop Canada Inc., Burlington, ON, Canada), 2.5 m m sodium pyrophosphate (Cell Signaling Technology), and 1 m m β-glycerophosphate (Cell Signaling Technology). .. Whole organisms were also treated with 5% NAC (Sigma-Aldrich, St. Louis, MO) in 1× PBS (BioShop) for 8 min to remove their mucous coating , and subsequently lysed as described above.

Protease Inhibitor:

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

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    Cell Signaling Technology Inc m sodium pyrophosphate
    M Sodium Pyrophosphate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m sodium pyrophosphate/product/Cell Signaling Technology Inc
    Average 91 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    m sodium pyrophosphate - by Bioz Stars, 2020-09
    91/100 stars
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