m sodium β glycerophosphate  (Millipore)


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    Structured Review

    Millipore m sodium β glycerophosphate
    M Sodium β Glycerophosphate, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m sodium β glycerophosphate/product/Millipore
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m sodium β glycerophosphate - by Bioz Stars, 2020-09
    88/100 stars

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    Sonication:

    Article Title: Transcription Factor GATA4 Inhibits Doxorubicin-induced Autophagy and Cardiomyocyte Death *
    Article Snippet: .. Cardiomyocytes were lysed and sonicated in IP buffer: 40 m m Tris-HCl (pH 7.4), 137 m m NaCl, 25 m m sodium β-glycerophosphate, 2 m m sodium pyrophosphate, 2 m m EDTA, 1 m m sodium vanadate, 10% glycerol containing 1% Triton X-100, and Protease Inhibitor Mixture (Sigma). .. For immunoprecipitation, supernatants containing 100 μg of proteins were incubated with anti-Bcl2 monoclonal antibody (Santa Cruz) for 16 h followed by incubation with Protein A/G-agarose beads (Santa Cruz) for 1 h. Beads were washed with IP buffer three times.

    Protease Inhibitor:

    Article Title: Transcription Factor GATA4 Inhibits Doxorubicin-induced Autophagy and Cardiomyocyte Death *
    Article Snippet: .. Cardiomyocytes were lysed and sonicated in IP buffer: 40 m m Tris-HCl (pH 7.4), 137 m m NaCl, 25 m m sodium β-glycerophosphate, 2 m m sodium pyrophosphate, 2 m m EDTA, 1 m m sodium vanadate, 10% glycerol containing 1% Triton X-100, and Protease Inhibitor Mixture (Sigma). .. For immunoprecipitation, supernatants containing 100 μg of proteins were incubated with anti-Bcl2 monoclonal antibody (Santa Cruz) for 16 h followed by incubation with Protein A/G-agarose beads (Santa Cruz) for 1 h. Beads were washed with IP buffer three times.

    Article Title: Role of c‐Fos/JunD in protecting stress‐induced cell death
    Article Snippet: .. Following treatment, cells were scraped gently into the medium, were pelleted, washed in ice‐cold phosphate‐buffered saline and were lysed on ice in four volumes of homogenization buffer [10 m m Hepes‐NaOH buffer (pH 7.9), 10 m m KCl, 1 m m EDTA, 1 m m ethylene glycol‐bis(2‐aminoethyl ether) N , N , N ′ N ′‐tetraacetic acid (EGTA), 10 m m sodium fluoride, 10 m m sodium‐β‐glycerophosphate, 5 m m dithiothreitol (DTT) and the CompleteTM protease inhibitor cocktail (Sigma‐Aldrich Inc.)]. .. Following addition of Nonidet P‐40 at a final concentration of 0.6%, the homogenates were vortexed for 10 s constantly and were then incubated for 15 min on ice.

    Article Title: Lysyl Oxidase Oxidizes Cell Membrane Proteins and Enhances the Chemotactic Response of Vascular Smooth Muscle Cells *
    Article Snippet: .. The reaction was stopped by aspirating the media, and cells were immediately lysed with 200 μl/well extraction buffer containing 20 m m Tris, pH 7.5, 5 m m EGTA, 150 m m NaCl, 1% Nonidet P-40, 0.1 m m Na3 VO4 , 1 m m NaF, 10 m m sodium β-glycerophosphate, 0.5 m m phenylmethylsulfonyl fluoride, 1 μl/ml protease inhibitor mixture (Sigma). ..

    Article Title: Cell Signaling, Internalization, and Nuclear Localization of the Angiotensin Converting Enzyme in Smooth Muscle and Endothelial Cells *
    Article Snippet: .. The reaction was stopped by aspirating the medium, and cells were immediately lysed with 200 μl/well of extraction buffer containing 20 m m Tris, pH 7.5, 5 m m EGTA, 150 m m NaCl, 1% Nonidet P-40, 0.1 m m Na3 VO4 , 1 m m sodium fluoride, 10 m m sodium β-glycerophosphate, 0.5 m m phenylmethylsulfonyl fluoride, 1 μl/ml of protease inhibitor mixture (Sigma). .. Cell extracts were sonicated to break down genomic DNA, clarified by centrifugation at 10,000 × g for 10 min at 4 °C, and the supernatants were saved.

    Homogenization:

    Article Title: Role of c‐Fos/JunD in protecting stress‐induced cell death
    Article Snippet: .. Following treatment, cells were scraped gently into the medium, were pelleted, washed in ice‐cold phosphate‐buffered saline and were lysed on ice in four volumes of homogenization buffer [10 m m Hepes‐NaOH buffer (pH 7.9), 10 m m KCl, 1 m m EDTA, 1 m m ethylene glycol‐bis(2‐aminoethyl ether) N , N , N ′ N ′‐tetraacetic acid (EGTA), 10 m m sodium fluoride, 10 m m sodium‐β‐glycerophosphate, 5 m m dithiothreitol (DTT) and the CompleteTM protease inhibitor cocktail (Sigma‐Aldrich Inc.)]. .. Following addition of Nonidet P‐40 at a final concentration of 0.6%, the homogenates were vortexed for 10 s constantly and were then incubated for 15 min on ice.

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    Millipore phosphatase inhibitors
    Phosphatase Inhibitors, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 2524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2524 article reviews
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