m phenylmethylsulfonyl fluoride pmsf  (Millipore)


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  • 99
    Name:
    PMSF
    Description:

    Catalog Number:
    pmsf-ro
    Price:
    None
    Applications:
    PMSF (C7H7O2SF) inhibits serine proteases such as chymotrypsin, trypsin, thrombin, and the cysteine protease papain (reversible by DTT treatment). It does not inhibit metalloproteases, most cysteine proteases, or aspartic proteases.PMSF (phenylmethylsulfonyl fluoride) has been used for the preparation of protein extracts from tissues and cells prior to Western blotting.
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    Structured Review

    Millipore m phenylmethylsulfonyl fluoride pmsf
    PMSF

    https://www.bioz.com/result/m phenylmethylsulfonyl fluoride pmsf/product/Millipore
    Average 99 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Electrophoresis:

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Protease Inhibitor:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Neutrophil activation and arteritis induced by C. albicans water-soluble mannoprotein-β-glucan complex (CAWS)
    Article Snippet: .. Reagents ELISA kits for mouse IL-1β, IL-6, IL-10, IL-12 p70, IFN-γ, TNF-α were purchased from BD Biosciences (CA, USA), IL-18 was from Medical and Biological Laboratories (Nagoya, Japan), soluble ICAM-1 and MIP-2 were from R & D Systems (MN, USA), G-CSF and GM-CSF were from AN'ALYZA (MN, US). fMet-Leu-Phe (fMLP) was purchased from Peptide Institute (Osaka, Japan), 3,3′, 5,5′-tetrametylbendizine (TMB), cytochalasin B (CB), cytochrome c , RPMI 1640 medium, aprotinin and PMSF were purchased from Sigma Chemical Co. (MO, USA). ..

    Incubation:

    Article Title: A Novel Pathway for Mammary Epithelial Cell Invasion Induced by the Helix-Loop-Helix Protein Id-1
    Article Snippet: .. Concentrated conditioned medium was mixed with nonreducing Laemmli sample buffer and incubated at 37°C for 15 min. After electrophoresis, the gels were incubated for 1 h in 2.5% Triton X-100 at room temperature, followed by 24 to 48 h in substrate buffer (100 mM Tris-HCl [pH 7.4]–15 mM CaCl2 ) in the absence or presence of GM6001 (0.2 mM in DMSO; supplied by Glycomed Corporation and obtained from Z. Werb [ ]), EDTA (10 mM), ortho -phenanthroline (1 mM in DMSO; Sigma), PMSF (5 mM), or AEBSF (0.5 mM; Calbiochem). .. The gels were stained with Coomassie blue for 30 min and were destained with 30% methanol–10% acetic acid.

    Inhibition:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Activity Assay:

    Article Title: Ku86 exists as both a full-length and a protease-sensitive natural variant in multiple myeloma cells
    Article Snippet: .. Intracellular inhibition of protease digestion In order to inhibit intracellular protease activity, 5 × 106 cells/sample were treated with: Complete™ protease inhibitor tablets; either 1× (1 tablet for every 50 mL of media) or 2× (2 tablets for every 50 mL of media); or treated with antipain (2.0 μg/mL) plus leupeptin (2.0 μg/mL) (both from Sigma-Aldrich, St Louis, MO, USA) for cysteine protease inhibition; or aprotinin (2.0 μg/mL) plus PMSF (100 μg/mL) (both from Sigma-Aldrich) for serine protease inhibition; for to 24 hrs. ..

    Lysis:

    Article Title: Main constraints for RNAi induced by expressed long dsRNA in mouse cells
    Article Snippet: .. Before collection, the cells were washed with PBS and lysed in lysis buffer (20 mM Hepes [pH 7.8], 100 mM NaCl, 1 mM EDTA [pH 8.0], 0.5% IGEPAL-25%, 1 mM fresh DTT, 0.5 mM PMSF, 1 mM NaF, 0.2 mM Na3 VO4 , supplemented with 2× protease inhibitor cocktail set [Millipore], 2× phosphatase inhibitor cocktail set [Millipore], and RiboLock RNase inhibitor [Thermo Fisher Scientific]). .. Proteins were separated on 10% polyacrylamide gel and transferred to a PVFD membrane (Millipore).

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  • Bioz Stars
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  • 99
    Millipore m pmsf
    M Pmsf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m pmsf/product/Millipore
    Average 99 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    m pmsf - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Millipore m phenylmethylsulfonyl fluoride pmsf
    M Phenylmethylsulfonyl Fluoride Pmsf, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m phenylmethylsulfonyl fluoride pmsf/product/Millipore
    Average 91 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m phenylmethylsulfonyl fluoride pmsf - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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