hydrogen peroxide preparation hydrogen peroxide solution  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Hydrogen peroxide solution
    Description:

    Catalog Number:
    h1009
    Price:
    None
    Applications:
    Used to prepare solutions for induction of apoptosis. ~60% of HL60 cells were apoptotic after exposure to 15 μM H2O2 for 8 hr.
    Buy from Supplier


    Structured Review

    Millipore hydrogen peroxide preparation hydrogen peroxide solution
    Hydrogen peroxide solution

    https://www.bioz.com/result/hydrogen peroxide preparation hydrogen peroxide solution/product/Millipore
    Average 99 stars, based on 867 article reviews
    Price from $9.99 to $1999.99
    hydrogen peroxide preparation hydrogen peroxide solution - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "In Vitro Assessment of Tobacco Smoke Toxicity at the BBB: Do Antioxidant Supplements Have a Protective Role?"

    Article Title: In Vitro Assessment of Tobacco Smoke Toxicity at the BBB: Do Antioxidant Supplements Have a Protective Role?

    Journal: BMC Neuroscience

    doi: 10.1186/1471-2202-12-92

    Exposure to hydrogen peroxide affects BBB viability and integrity . (Panel A) Under the exposure to pulsatile flow in presence of abluminal astrocytes the vascular endothelium formed a stable highly impermeable barrier (TEER ≈ 1200 Ohm/cm 2 ) ( Panel A left side). Exposure to H 2 O 2 equivalent to that contained in 10 and 20 cigarettes determined a loss of BBB integrity ( Panel A right side). Loss of BBB integrity and viability at the highest H 2 O 2 level of exposure was partially prevented to a different extent by pre-treatment with antioxidant vitamins (C, E, and combined treatment, Panel B ). The described experiments were performed in quadruplicates (n = 4). The asterisk
    Figure Legend Snippet: Exposure to hydrogen peroxide affects BBB viability and integrity . (Panel A) Under the exposure to pulsatile flow in presence of abluminal astrocytes the vascular endothelium formed a stable highly impermeable barrier (TEER ≈ 1200 Ohm/cm 2 ) ( Panel A left side). Exposure to H 2 O 2 equivalent to that contained in 10 and 20 cigarettes determined a loss of BBB integrity ( Panel A right side). Loss of BBB integrity and viability at the highest H 2 O 2 level of exposure was partially prevented to a different extent by pre-treatment with antioxidant vitamins (C, E, and combined treatment, Panel B ). The described experiments were performed in quadruplicates (n = 4). The asterisk "*" indicates a statistically significant difference (p

    Techniques Used: Flow Cytometry

    2) Product Images from "Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction"

    Article Title: Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

    Journal: Stem Cells International

    doi: 10.1155/2015/176409

    Antiapoptotic effect of hBcl-xL-MSCs in vitro . (a) Annexin V-FITC/PI apoptosisassay of wild type MSCs, vector-MSCs, and hBcl-xL-MSCs. Cells were seeded into T25 flasks and were cultured in medium with 200 μ M H 2 O 2 at 37°C for 4 hours. The resuspended cells were incubated with Annexin V-FITC and PI for 15 min and checked with a BD FASAria Cell Sorter. (b) The apoptotic rate was presented as mean ± SD ( n = 3, ∗ P
    Figure Legend Snippet: Antiapoptotic effect of hBcl-xL-MSCs in vitro . (a) Annexin V-FITC/PI apoptosisassay of wild type MSCs, vector-MSCs, and hBcl-xL-MSCs. Cells were seeded into T25 flasks and were cultured in medium with 200 μ M H 2 O 2 at 37°C for 4 hours. The resuspended cells were incubated with Annexin V-FITC and PI for 15 min and checked with a BD FASAria Cell Sorter. (b) The apoptotic rate was presented as mean ± SD ( n = 3, ∗ P

    Techniques Used: In Vitro, Plasmid Preparation, Cell Culture, Incubation

    3) Product Images from "Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction"

    Article Title: Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

    Journal: Stem Cells International

    doi: 10.1155/2015/176409

    Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P
    Figure Legend Snippet: Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P

    Techniques Used: Plasmid Preparation, Transplantation Assay, Staining, Injection

    4) Product Images from "Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction"

    Article Title: Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

    Journal: Stem Cells International

    doi: 10.1155/2015/176409

    Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P
    Figure Legend Snippet: Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P

    Techniques Used: Plasmid Preparation, Transplantation Assay, Staining, Injection

    5) Product Images from "Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction"

    Article Title: Bcl-xL Genetic Modification Enhanced the Therapeutic Efficacy of Mesenchymal Stem Cell Transplantation in the Treatment of Heart Infarction

    Journal: Stem Cells International

    doi: 10.1155/2015/176409

    Upregulation of angiogenic cytokines in hBcl-xL-MSCs. ((a)–(c)) Evaluation of the paracrine secretion of VEGF (a), IGF-1 (b), and PDGF (c) by wild type MSCs (pale grey bar), vector-MSCs (dark grey bar), and hBcl-xL-MSCs (black bar). Cells were cultured under either normoxic or hypoxic conditions for 24 hours and the conditioned medium was collected for ELISA. Concentration values are mean ± SD ( n = 3, ∗ P
    Figure Legend Snippet: Upregulation of angiogenic cytokines in hBcl-xL-MSCs. ((a)–(c)) Evaluation of the paracrine secretion of VEGF (a), IGF-1 (b), and PDGF (c) by wild type MSCs (pale grey bar), vector-MSCs (dark grey bar), and hBcl-xL-MSCs (black bar). Cells were cultured under either normoxic or hypoxic conditions for 24 hours and the conditioned medium was collected for ELISA. Concentration values are mean ± SD ( n = 3, ∗ P

    Techniques Used: Plasmid Preparation, Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay

    Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P
    Figure Legend Snippet: Engraftment of transplanted MSCs into infarcted myocardium. ((a)–(n)) Evaluation of MSC engraftment into the ischemic heart muscle at 1 week ((a)–(f)) and 4 weeks ((g)–(n)) after vector-MSCs ((a)–(c) and (g)–(j)) or hBcl-xL-MSCs ((d)–(f) and (k)–(n)) transplantation. Cell nuclei were stained with DAPI ((a), (d), (g), and (k)). The grafted MSCs were detected by EGFP signals ((b), (e), (h), and (l)) which appeared as irregular patches due to the cytoplasmic distribution of EGFP. The expanded squared areas in (c), (f), (j), and (n) indicate the MSC engraftments where the EGFP signals colocalized with DAPI. Myocardium was showed by Troponin T staining 4 weeks after cell injection ((i) and (m)). (o) MSC engraftment in vector-MSC group (dark grey bar) and hBcl-xL-MSC group (black bar) was presented as the ratio of IOD and total cell number per HPF. Values are mean ± SD ( n = 6, ∗ P

    Techniques Used: Plasmid Preparation, Transplantation Assay, Staining, Injection

    The surface marker expression of genetically modified rat MSCs. The expressions of selected surface markers of the modified MSCs were analyzed by flow cytometry. Mouse IgG1 was used as an isotype control (a); in the genetically modified MSCs, CD29, CD90, and CD44 were highly expressed ((b)–(d)) while the markers for hematopoietic stem cells, CD34 and CD45, were not expressed ((e) and (f)).
    Figure Legend Snippet: The surface marker expression of genetically modified rat MSCs. The expressions of selected surface markers of the modified MSCs were analyzed by flow cytometry. Mouse IgG1 was used as an isotype control (a); in the genetically modified MSCs, CD29, CD90, and CD44 were highly expressed ((b)–(d)) while the markers for hematopoietic stem cells, CD34 and CD45, were not expressed ((e) and (f)).

    Techniques Used: Marker, Expressing, Genetically Modified, Modification, Flow Cytometry, Cytometry

    hBcl-xL-MSCs promoted angiogenesis and prevented scar formation in infarcted heart. ((a)–(c)) Evaluation of capillary density by von Willebrand factor staining at 4 weeks after transplantation of medium (a), vector-MSCs (b), or hBcl-xL-MSCs (c). ((d)–(f)) Assessment of scar formation by Trichrome-Masson staining at 4 weeks after medium (d), vector-MSCs (e), or hBcl-xL-MSCs (f) transplantation. (g) Capillary density was presented as the number of vessels per high-power field. (h) Scar size was presented as the percentage of the collagen deposition area to the whole section area of the left ventricle. Values are mean ± SD ( n = 6, ∗ P
    Figure Legend Snippet: hBcl-xL-MSCs promoted angiogenesis and prevented scar formation in infarcted heart. ((a)–(c)) Evaluation of capillary density by von Willebrand factor staining at 4 weeks after transplantation of medium (a), vector-MSCs (b), or hBcl-xL-MSCs (c). ((d)–(f)) Assessment of scar formation by Trichrome-Masson staining at 4 weeks after medium (d), vector-MSCs (e), or hBcl-xL-MSCs (f) transplantation. (g) Capillary density was presented as the number of vessels per high-power field. (h) Scar size was presented as the percentage of the collagen deposition area to the whole section area of the left ventricle. Values are mean ± SD ( n = 6, ∗ P

    Techniques Used: Staining, Transplantation Assay, Plasmid Preparation

    Antiapoptotic effect of hBcl-xL-MSCs in vitro . (a) Annexin V-FITC/PI apoptosisassay of wild type MSCs, vector-MSCs, and hBcl-xL-MSCs. Cells were seeded into T25 flasks and were cultured in medium with 200 μ M H 2 O 2 at 37°C for 4 hours. The resuspended cells were incubated with Annexin V-FITC and PI for 15 min and checked with a BD FASAria Cell Sorter. (b) The apoptotic rate was presented as mean ± SD ( n = 3, ∗ P
    Figure Legend Snippet: Antiapoptotic effect of hBcl-xL-MSCs in vitro . (a) Annexin V-FITC/PI apoptosisassay of wild type MSCs, vector-MSCs, and hBcl-xL-MSCs. Cells were seeded into T25 flasks and were cultured in medium with 200 μ M H 2 O 2 at 37°C for 4 hours. The resuspended cells were incubated with Annexin V-FITC and PI for 15 min and checked with a BD FASAria Cell Sorter. (b) The apoptotic rate was presented as mean ± SD ( n = 3, ∗ P

    Techniques Used: In Vitro, Plasmid Preparation, Cell Culture, Incubation

    Expressions of EGFP and hBcl-xL in rat bone marrow MSCs. (a) Schematic representation of the coding regions of the viral vectors pLenti6.3-IRES-EGFP and pLenti6.3-hBcl-xL-IRES-EGFP. In vector pLenti6.3-hBcl-xL-IRES-EGFP, the insertion of IRES could lead to the individual expression of EGFP together with the expression of hBcl-xL in the transduced cells. All expressions were driven by CMV promoter. ((b)–(g)) Expressions of EGFP in wild type MSCs ((b) and (c)), vector-MSCs ((d) and (e)), and hBcl-xL-MSCs ((f) and (g)). (h) Western blot analysis of the expressions of hBcl-xL in MSCs, vector-MSCs, and hBcl-xL-MSCs. Data are representative of three independent experiments.
    Figure Legend Snippet: Expressions of EGFP and hBcl-xL in rat bone marrow MSCs. (a) Schematic representation of the coding regions of the viral vectors pLenti6.3-IRES-EGFP and pLenti6.3-hBcl-xL-IRES-EGFP. In vector pLenti6.3-hBcl-xL-IRES-EGFP, the insertion of IRES could lead to the individual expression of EGFP together with the expression of hBcl-xL in the transduced cells. All expressions were driven by CMV promoter. ((b)–(g)) Expressions of EGFP in wild type MSCs ((b) and (c)), vector-MSCs ((d) and (e)), and hBcl-xL-MSCs ((f) and (g)). (h) Western blot analysis of the expressions of hBcl-xL in MSCs, vector-MSCs, and hBcl-xL-MSCs. Data are representative of three independent experiments.

    Techniques Used: Plasmid Preparation, Expressing, Western Blot

    Antiapoptotic effect of hBcl-xL-MSCs in vivo . ((a)–(c)) TUNEL assay was performed at 4 weeks after transplantation of medium (a), vector-MSCs (b), or hBcl-xL-MSCs (c). TUNEL-positive cells were defined as cells with clear brown-colored nuclear labeling. (d) The apoptotic rate was presented as the ratio of the area of TUNEL-positive cell nuclei and the area of total cell nuclei. Values are mean ± SD ( n = 6, ∗ P
    Figure Legend Snippet: Antiapoptotic effect of hBcl-xL-MSCs in vivo . ((a)–(c)) TUNEL assay was performed at 4 weeks after transplantation of medium (a), vector-MSCs (b), or hBcl-xL-MSCs (c). TUNEL-positive cells were defined as cells with clear brown-colored nuclear labeling. (d) The apoptotic rate was presented as the ratio of the area of TUNEL-positive cell nuclei and the area of total cell nuclei. Values are mean ± SD ( n = 6, ∗ P

    Techniques Used: In Vivo, TUNEL Assay, Transplantation Assay, Plasmid Preparation, Labeling

    Related Articles

    Concentration Assay:

    Article Title: Effects of Chrysotile Exposure in Human Bronchial Epithelial Cells: Insights into the Pathogenic Mechanisms of Asbestos-Related Diseases
    Article Snippet: .. Specific Inhibitors The neutralizing anti–TGF-β antibody was purchased from Abcam and was used at a concentration of 5 μg/mL; the GSK-3β inhibitor SB 216763 and the Akt 1/2 kinase inhibitor were from Sigma and were both used at a concentration of 5 μM. .. Quantitative Real-Time PCR (qRT-PCR) Total RNA was obtained by the guanidinium thiocyanate–phenol–chloroform method , using RiboZol RNA Extraction Reagents (Amresco)according to the manufacturer’s instructions.

    Article Title: Wnt Signaling Regulates the Lineage Differentiation Potential of Mouse Embryonic Stem Cells through Tcf3 Down-Regulation
    Article Snippet: .. The Gsk-inhibitor SB-216763 was purchased from Sigma, dissolved in DMSO and used at 10 µM final concentration. .. The Gsk-inhibitor SB-216763 was purchased from Sigma, dissolved in DMSO and used at 10 µM final concentration.

    other:

    Article Title: Role of MCP-1 and CCR2 in ethanol-induced neuroinflammation and neurodegeneration in the developing brain
    Article Snippet: GSK3β inhibitor SB-216763 was purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA).

    Article Title: In Vitro Assessment of Tobacco Smoke Toxicity at the BBB: Do Antioxidant Supplements Have a Protective Role?
    Article Snippet: Hydrogen peroxide preparation Hydrogen peroxide solution (PERDROGEN® 30% H2 O2 ) was purchased from Sigma-Aldrich (St. Louis, MO 63178; cat# 31642) and diluted to achieve the appropriate final experimental concentrations (55, 220, and 385 μM respectively which are comparable to that yield by 5, 20, and 35 2R24 research cigarettes; 2-4 μM/mg of tar [ , ]).

    In Vitro:

    Article Title: Inhibition of GSK3α/β impairs the progression of HNSCC
    Article Snippet: .. Reagents The specific GSK3α/β inhibitor SB 216763 (10 and 30 µM, Sigma-Aldrich St. Louis, MO, USA) is a structurally distinct maleimide which inhibits GSK3α in vitro in an ATP-competitive manner with an IC50 value of 34 nM. ..

    Article Title: Inhibition of GSK3α/β impairs the progression of HNSCC
    Article Snippet: .. The specific GSK3α/β inhibitor SB 216763 (10 and 30 µM, Sigma-Aldrich St. Louis, MO, USA) is a structurally distinct maleimide which inhibits GSK3α in vitro in an ATP-competitive manner with an IC50 value of 34 nM. ..