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Merck KGaA m nacl
M Nacl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m nacl/product/Merck KGaA
Average 92 stars, based on 11 article reviews
Price from $9.99 to $1999.99
m nacl - by Bioz Stars, 2021-01
92/100 stars

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Protease Inhibitor:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Blocking Assay:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Enzyme-linked Immunosorbent Assay:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Concentration Assay:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Incubation:

Article Title: Activation of the cGMP Pathway in Dopaminergic Structures Reduces Cocaine-Induced EGR-1 Expression and Locomotor Activity
Article Snippet: .. Sections were incubated in 10 m m Tris-HCl buffer, pH 8.0, containing 100 m m NaCl, 1 m m EDTA, and 6.10-3 U/ml RNase A (Merck, Darmstadt, Germany) for 30 min at 37°C. .. The sections were exposed to x-ray film (Biomax-MR; Eastman Kodak, Rochester, NY) for 6 d. For quantitative analysis, densitometry was performed with an image analyzer and Samba software (Alcatel TITN, Massy, France) on comparable areas of brain structures.

Article Title: Cell Cycle Modulation in the Response of the Primary Root of Arabidopsis to Salt Stress 1
Article Snippet: .. Subsequently, they were rinsed three times in Tris buffer (100 m m Tris [USB, Cleveland] with 50 m m NaCl [Merck Eurolab, Leuven, Belgium], pH 7.4), incubated for 30 min at 37°C in ferricyanate buffer (Tris buffer containing 0.5 m m ferricyanide [Sigma, St. Louis]) and placed in staining buffer (Tris buffer, containing 0.5 m m ferricyanide, 1 mg/mL 5-bromo-4-chloro-3-indolyly- β - d -GlcUA [UCB, Leuven, Belgium]) until blue staining was visible. .. Afterward they were rinsed three times in Tris buffer, cleared in lactic acid, and mounted on microscopy slides.

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Lysis:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Staining:

Article Title: Cell Cycle Modulation in the Response of the Primary Root of Arabidopsis to Salt Stress 1
Article Snippet: .. Subsequently, they were rinsed three times in Tris buffer (100 m m Tris [USB, Cleveland] with 50 m m NaCl [Merck Eurolab, Leuven, Belgium], pH 7.4), incubated for 30 min at 37°C in ferricyanate buffer (Tris buffer containing 0.5 m m ferricyanide [Sigma, St. Louis]) and placed in staining buffer (Tris buffer, containing 0.5 m m ferricyanide, 1 mg/mL 5-bromo-4-chloro-3-indolyly- β - d -GlcUA [UCB, Leuven, Belgium]) until blue staining was visible. .. Afterward they were rinsed three times in Tris buffer, cleared in lactic acid, and mounted on microscopy slides.

Binding Assay:

Article Title: Redox-Sensitive Structure and Function of the First Extracellular Loop of the Cell–Cell Contact Protein Claudin-1: Lessons from Molecular Structure to Animals
Article Snippet: .. Human Cld131–81 , with N-terminal GST- or MBP-tag , was expressed in Escherichia coli , harvested, and resuspended in binding buffer, for MBP-constructs, 20 m M Tris/HCl, pH 7.4, 200 m M NaCl, 1 m M ethylenediaminetetraacetic acid (EDTA; Merck, Darmstadt, Germany) , and for GST-constructs, 20 m M Tris/HCl, pH 7.9, 125 m M NaCl, 5 m M MgCl2 , 10% glycerol, 0.1% TX-100, 0.1 m M phenylmethanesulfonyl fluoride (Sigma-Aldrich, Taufkirchen, Germany), and 0.5 m M DTT (Bio-Rad, Munich, Germany). .. After sonication (300 W) and centrifugation (39,000 g ), the supernatant was incubated (1 h, 4°C) with amylose resin (MBP-proteins; NEB, Ipswich, MA) and glutathione sepharose 4B (GST-proteins; GE Healthcare, Buckinghamshire, United Kingdom) to immobilize and purify the constructs.

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  • 92
    Merck KGaA m nacl
    M Nacl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m nacl/product/Merck KGaA
    Average 92 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    m nacl - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

    88
    Merck KGaA 5 m nacl
    5 M Nacl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/5 m nacl/product/Merck KGaA
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    5 m nacl - by Bioz Stars, 2021-01
    88/100 stars
      Buy from Supplier

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