Structured Review

Merck & Co m nacl
M Nacl, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m nacl/product/Merck & Co
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m nacl - by Bioz Stars, 2021-01
92/100 stars

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Centrifugation:

Article Title: Structure–function analyses of the bacterial zinc metalloprotease effector protein GtgA uncover key residues required for deactivating NF-κB
Article Snippet: .. Proteins were dialyzed overnight at 4 °C into 25 m m HEPES, pH 7.5, 150 m m NaCl, and 0.5 m m TCEP before concentration to ∼5 mg/ml using Amicon Ultra-15 (MWCO 10 kDa) centrifugation filters (Merck). .. For crystallization, proteins were eluted from the resin following overnight incubation with 0.5 m m TCEP and TEV protease or the SUMO protease ULP1.

Article Title: Purification, crystallization and preliminary X-ray diffraction of SecDF, a translocon-associated membrane protein, from Thermus thermophilus
Article Snippet: .. Cells were then precultured at 310 K in 100 ml LB medium supplemented with 50 µg ml−1 ampicillin, 20 µg ml−1 chloramphenicol and 0.4%( w / v ) glucose and were inoculated into 10 l LB medium containing the same antibiotics and 1 m M isopropyl 1-thio-β- d -galactopyranoside for additional growth at 310 K for 12 h. Cells were harvested by centrifugation and total membranes were prepared as described previously (Matsuo et al. , 1998 ) and solubilized with 20 m M Tris–HCl buffer pH 8.1 containing 2%( w / v ) n -dodecyl-β-maltoside (DDM), 5%( v / v ) glycerol, 300 m M NaCl, 20 m M imidazole pH 7.0 and 0.1 m M Pefabloc (Merck) by gentle mixing for 30 min at 277 K. After the removal of insoluble materials by ultracentrifugation, the supernatant was applied onto an Ni–NTA column equilibrated with buffer A [20 m M Tris–HCl pH 8.1, 0.1%( w / v ) DDM, 5%( v / v ) glycerol, 300 m M NaCl and 0.1 m M Pefabloc]. ..

Protease Inhibitor:

Article Title: Transcriptional regulators involved in responses to volatile organic compounds in plants
Article Snippet: .. Total protein was extracted from crushed leaves in an extraction buffer containing 50 m m Tris-HCl (pH 7.4), 100 m m NaCl, 5 m m KCl, 1 m m DTT, 10 μ m MG132 proteasome inhibitor (Merck), and the cOmplete, Mini, EDTA-free protease inhibitor mixture tablet (Roche Applied Science) according to the supplier's instructions. ..

Article Title: Transcriptional regulators involved in responses to volatile organic compounds in plants
Article Snippet: .. After incubation, beads were separated by a magnet (Veritas) from the supernatant and washed three times with protein extraction buffer containing 50 m m Tris-HCl (pH 7.4), 100 m m NaCl, 5 m m KCl, 1 m m DTT, 10 μ m MG132 proteasome inhibitor (Merck), and the cOmplete, Mini, EDTA-free protease inhibitor mixture tablet (Roche Applied Science) according to the supplier's instructions. .. SDS-PAGE and silver staining were carried out to confirm the size of the proteins and their binding activities.

Incubation:

Article Title: Transcriptional regulators involved in responses to volatile organic compounds in plants
Article Snippet: .. After incubation, beads were separated by a magnet (Veritas) from the supernatant and washed three times with protein extraction buffer containing 50 m m Tris-HCl (pH 7.4), 100 m m NaCl, 5 m m KCl, 1 m m DTT, 10 μ m MG132 proteasome inhibitor (Merck), and the cOmplete, Mini, EDTA-free protease inhibitor mixture tablet (Roche Applied Science) according to the supplier's instructions. .. SDS-PAGE and silver staining were carried out to confirm the size of the proteins and their binding activities.

Concentration Assay:

Article Title: Structure–function analyses of the bacterial zinc metalloprotease effector protein GtgA uncover key residues required for deactivating NF-κB
Article Snippet: .. Proteins were dialyzed overnight at 4 °C into 25 m m HEPES, pH 7.5, 150 m m NaCl, and 0.5 m m TCEP before concentration to ∼5 mg/ml using Amicon Ultra-15 (MWCO 10 kDa) centrifugation filters (Merck). .. For crystallization, proteins were eluted from the resin following overnight incubation with 0.5 m m TCEP and TEV protease or the SUMO protease ULP1.

Protein Extraction:

Article Title: Transcriptional regulators involved in responses to volatile organic compounds in plants
Article Snippet: .. After incubation, beads were separated by a magnet (Veritas) from the supernatant and washed three times with protein extraction buffer containing 50 m m Tris-HCl (pH 7.4), 100 m m NaCl, 5 m m KCl, 1 m m DTT, 10 μ m MG132 proteasome inhibitor (Merck), and the cOmplete, Mini, EDTA-free protease inhibitor mixture tablet (Roche Applied Science) according to the supplier's instructions. .. SDS-PAGE and silver staining were carried out to confirm the size of the proteins and their binding activities.

Lysis:

Article Title: Two Arabidopsis Genes (IPMS1 and IPMS2) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W]) Encode Isopropylmalate Synthase, the Branchpoint Step in the Biosynthesis of Leucine 1 [W] [OA]
Article Snippet: .. A bacterial pellet was homogenized in a 2-mL Eppendorf tube with 1.5 mL of lysis buffer, containing 50 m m sodium borate buffer, pH 8.0, 300 m m NaCl, 10 m m imidazole, and 1 m m MgCl2 , and left on ice for 30 min after the addition of 1.0 × 105 units lysozyme (Merck). ..

Sonication:

Article Title:
Article Snippet: .. Briefly, 25 μ m sonicated phospholipid vesicles comprising 15% phosphatidylserine, 20% phosphatidylethanolamine, and 65% phosphatidylcholine were mixed with 0.3 n m FVIII, 200 n m FX, and 0–16 n m FIXa in a buffer containing 40 m m Tris-HCl (pH 7.8), 150 m m NaCl, 0.2% (w/v) bovine serum albumin (BSA) (Merck). ..

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    Merck & Co m nacl
    M Nacl, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m nacl/product/Merck & Co
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m nacl - by Bioz Stars, 2021-01
    92/100 stars
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