m nacl (Fisher Scientific)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Fisher Scientific m nacl
M Nacl, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m nacl/product/Fisher Scientific
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99

m nacl - by Bioz Stars, 2021-01

92/100 stars

Related Products / Commonly Used Together

m tris-hcl
m edta
m tris
triton x-100
nonidet p-40
m hepes
protease inhibitor mixture
m mgcl2
m dtt
sds
protease inhibitors
sodium deoxycholate
m imidazole
m egta
pbs

Images

Lysis:

Article Title: Characterization of the SRP68/72 interface of human signal recognition particle by systematic site-directed mutagenesis
Article Snippet: .. Frozen cells were resuspended in 6.5 mL of ice-cold 50 m M Na-phosphate, pH 8.0, 200 m M NaCl, 5 m M EDTA, 5 m M DTT (lysis buffer) and sonicated, using a Sonic Dismembrator Model 300 (Fisher Scientific) at a setting of 35 percent, three times for 15 s with 15 s intervals on ice. .. The lysate was subjected at 4°C to a low-speed centrifugation at 15,000 rpm using a Sorvall SS34 rotor for 20 min.

Article Title: Neutral Sphingomyelinase 2 Activity and Protein Stability Are Modulated by Phosphorylation of Five Conserved Serines *
Article Snippet: .. Cells were collected by scraping either in PBS or directly in the lysis buffer: 1% Nonidet P-40 (IGEPAL, from Sigma), 50 m m Tris, 10% glycerol, 0.02% NaN3 , 150 m m NaCl, pH 7.4 (all from Fisher Chemical), containing a mixture of phosphatase and protease inhibitors (Sigma) as well as 1 m m NaF and Na3 VO4 (both from Sigma). .. Lysates were passed five times through a 30-gauge needle prior to centrifugation and further processing of the samples.

Centrifugation:

Article Title: Identification of a metabolic disposal route for the oncometabolite S-(2-succino)cysteine in Bacillus subtilis
Article Snippet: .. Cells were harvested by centrifugation (8000 × g , 10 min); resuspended in 5 ml of 50 m m potassium phosphate, pH 8.0, 300 m m NaCl, and 10 m m imidazole; and sonicated (Fisher Scientific Ultrasonic Dismembrator, model 150E) for 5 × 15-s pulses at 70% power, cooling on ice for 60 s between pulses. .. The lysate was centrifuged at 17,000 × g for 10 min, and the supernatant was added to a column containing 0.25 ml of Ni2+ -nitrilotriacetic acid resin (Qiagen).

Chloramphenicol Acetyltransferase Assay:

Article Title: Host-centric Proteomics of Stool: A Novel Strategy Focused on intestinal Responses to the Gut Microbiota *
Article Snippet: .. Fecal pellets were cut on dry ice, weighed, suspended in 500 μl 8 m Urea (Invitrogen, Carlsbad, CA; CAS# 57–13-6), 100 m m NaCl (Fisher Scientific, CAS# 7467–14-5), 25 m m Tris (Amresco, Solon, Ohio; CAS# 77–86-1), pH 8.2 with protease inhibitors (Roche Complete, EDTA free, Cat05056489001). ..

Sonication:

Article Title: Programmed cell death in the marine cyanobacterium Trichodesmium mediates carbon and nitrogen export
Article Snippet: .. Surface filaments (15 ml) were collected onto 25-m M diameter, 5-μ M pore-size polycarbonate filters, placed in a 2-ml eppendorf tube and snap-frozen in liquid N. Cells were resuspended in Lauber buffer (50 m M HEPES (pH 7.3), 100 m M NaCl, 10% sucrose, 0.1% 3-[(3- cholamidopropyl)-dimethylammonio]-1 propanesulfonate and 10 m M dithiothreitol) and sonicated on ice (four times, each for a 30 S cycle) using an ultracell disruptor (Sonic Dismembrator, Fisher Scientific, Waltham, MA, USA). .. Cell extracts were centrifuged then (10 000 g , 2 min), and supernatant was collected for caspase activity and protein assays.