Structured Review

Roche m na3 vo4
M Na3 Vo4, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m na3 vo4/product/Roche
Average 91 stars, based on 75 article reviews
Price from $9.99 to $1999.99
m na3 vo4 - by Bioz Stars, 2020-07
91/100 stars

Images

Related Articles

Transfection:

Article Title: A Brain-specific Grb2-associated Regulator of Extracellular Signal-regulated Kinase (Erk)/Mitogen-activated Protein Kinase (MAPK) (GAREM) Subtype, GAREM2, Contributes to Neurite Outgrowth of Neuroblastoma Cells by Regulating Erk Signaling *
Article Snippet: .. The transfected cells were lysed in a lysis buffer containing 20 m m Tris-HCl (pH 7.5), 1 m m EDTA, 10 m m DTT, 1% Triton X-100, 150 m m NaCl, 10 m m NaF, 1 m m Na3 VO4 , and a complete protease inhibitor mixture (Roche) to produce a total cell lysate. .. For the immunoprecipitation experiments, the total cell lysate was centrifuged, and the supernatant was incubated for 2 h with either the primary antibody or an anti-FLAG affinity gel (Sigma).

Article Title: RACK1, a New ADAM12 Interacting Protein
Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

Protease Inhibitor:

Article Title: A Brain-specific Grb2-associated Regulator of Extracellular Signal-regulated Kinase (Erk)/Mitogen-activated Protein Kinase (MAPK) (GAREM) Subtype, GAREM2, Contributes to Neurite Outgrowth of Neuroblastoma Cells by Regulating Erk Signaling *
Article Snippet: .. The transfected cells were lysed in a lysis buffer containing 20 m m Tris-HCl (pH 7.5), 1 m m EDTA, 10 m m DTT, 1% Triton X-100, 150 m m NaCl, 10 m m NaF, 1 m m Na3 VO4 , and a complete protease inhibitor mixture (Roche) to produce a total cell lysate. .. For the immunoprecipitation experiments, the total cell lysate was centrifuged, and the supernatant was incubated for 2 h with either the primary antibody or an anti-FLAG affinity gel (Sigma).

Article Title: The Epsin Family of Endocytic Adaptors Promotes Fibrosarcoma Migration and Invasion *
Article Snippet: .. Plates were rinsed with 4 °C PBS and cells lysed with 400 μl/plate ice-cold lysis buffer: 50 m m Tris-HCl, pH 7.5, 1% IGEPAL, 100 m m KCl, 100 m m NaCl, 5 m m MgCl2 , 2 m m EGTA, 1 m m DTT, 1 m m Na3 VO4 , 1 m m NaF, and Complete Protease Inhibitor (Roche). ..

Article Title: Mutation of a Phosphorylatable Residue in Put3p Affects the Magnitude of Rapamycin-induced PUT1 Activation in a Gat1p-dependent Manner *
Article Snippet: .. Cells were initially washed, then resuspended in buffer A (20 m m HEPES-KOH (pH 7.8), 300 m m NaCl, 10% (v/v) glycerol) with the addition of 50 m m NaF, 20 m m β-glycerophosphate, 0.1 m m Na3 VO4 , and Roche-EDTA protease inhibitor tablets. ..

Article Title: Epigallocatechin Gallate (EGCG) Stimulates Autophagy in Vascular Endothelial Cells
Article Snippet: .. Cells were then scraped in lysis buffer containing 50 m m Tris (pH 7.2), 125 m m NaCl, 1% Triton X-100, 0.5% Nonidet P-40, 1 m m EDTA, 1 m m Na3 VO4 , 20 m m NaF, 1 m m sodium pyrophosphate, and complete protease inhibitor mixture (Roche Applied Science, 05056489001). .. Cell debris was pelleted by centrifugation at 17,000 × g for 10 min at 4 °C.

Article Title: Novel Markers of Left Ventricular Hypertrophy in Uremia
Article Snippet: .. Briefly, LV tissue was homogenized in RIPA buffer (50 m M Tris-HCl [pH 7.4]; 1% NP-40; 0.25% Na-deoxycholate, 150 m M NaCl; 1 m M Na3 VO4 , and 1 m M NaF) with 40 μl/ml of fresh protease inhibitor cocktail (Roche, Indianapolis, Ind., USA). .. Samples were mixed with sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) sample buffer and boiled for 5 min.

Article Title: Calcium-independent Phospholipase A2γ Enhances Activation of the ATF6 Transcription Factor during Endoplasmic Reticulum Stress *
Article Snippet: .. Cells were lysed in ice-cold buffer containing 1% Triton X-100, 125 m m NaCl, 10 m m Tris, pH 7.4, 1 m m EGTA, 2 m m Na3 VO4 , 10 m m sodium pyrophosphate, 25 m m NaF, and protease inhibitor mixture (Roche Diagnostics). .. Equal amounts of lysate proteins were dissolved in Laemmli buffer and were subjected to SDS-PAGE under reducing conditions.

Article Title: The Chaperone Activity of Heat Shock Protein 90 Is Critical for Maintaining the Stability of Leucine-Rich Repeat Kinase 2
Article Snippet: .. Cell were lysed in 20 m M Tris-HCl, pH 7.5, 10 m M NaCl, 1m M Na2 EDTA, 1 m M EGTA, 1% Triton X-100, 2.5 m M sodium pyrophosphate, 1 m M β -glycerophosphate, 1 m M Na3 VO4 , 1 mg/ml leupeptin, and protease inhibitor cocktail (Roche). .. The biotin-labeled LRRK2 was captured by streptavidin-coated particles and Magnesphere Paramagnetic particles (Promega) for 2 h at 4°C and then washed three times with PBS containing 150 m M NaCl and 1% Triton X-100 and once with PBS only.

Cell Culture:

Article Title: RACK1, a New ADAM12 Interacting Protein
Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

Immunoprecipitation:

Article Title: RACK1, a New ADAM12 Interacting Protein
Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

Expressing:

Article Title: RACK1, a New ADAM12 Interacting Protein
Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

Lysis:

Article Title: A Brain-specific Grb2-associated Regulator of Extracellular Signal-regulated Kinase (Erk)/Mitogen-activated Protein Kinase (MAPK) (GAREM) Subtype, GAREM2, Contributes to Neurite Outgrowth of Neuroblastoma Cells by Regulating Erk Signaling *
Article Snippet: .. The transfected cells were lysed in a lysis buffer containing 20 m m Tris-HCl (pH 7.5), 1 m m EDTA, 10 m m DTT, 1% Triton X-100, 150 m m NaCl, 10 m m NaF, 1 m m Na3 VO4 , and a complete protease inhibitor mixture (Roche) to produce a total cell lysate. .. For the immunoprecipitation experiments, the total cell lysate was centrifuged, and the supernatant was incubated for 2 h with either the primary antibody or an anti-FLAG affinity gel (Sigma).

Article Title: The Epsin Family of Endocytic Adaptors Promotes Fibrosarcoma Migration and Invasion *
Article Snippet: .. Plates were rinsed with 4 °C PBS and cells lysed with 400 μl/plate ice-cold lysis buffer: 50 m m Tris-HCl, pH 7.5, 1% IGEPAL, 100 m m KCl, 100 m m NaCl, 5 m m MgCl2 , 2 m m EGTA, 1 m m DTT, 1 m m Na3 VO4 , 1 m m NaF, and Complete Protease Inhibitor (Roche). ..

Article Title: Epigallocatechin Gallate (EGCG) Stimulates Autophagy in Vascular Endothelial Cells
Article Snippet: .. Cells were then scraped in lysis buffer containing 50 m m Tris (pH 7.2), 125 m m NaCl, 1% Triton X-100, 0.5% Nonidet P-40, 1 m m EDTA, 1 m m Na3 VO4 , 20 m m NaF, 1 m m sodium pyrophosphate, and complete protease inhibitor mixture (Roche Applied Science, 05056489001). .. Cell debris was pelleted by centrifugation at 17,000 × g for 10 min at 4 °C.

Western Blot:

Article Title: RACK1, a New ADAM12 Interacting Protein
Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91
    Roche m na3 vo4
    M Na3 Vo4, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Roche
    Average 91 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    97
    Roche ripa buffer
    Ripa Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 5816 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ripa buffer/product/Roche
    Average 97 stars, based on 5816 article reviews
    Price from $9.99 to $1999.99
    ripa buffer - by Bioz Stars, 2020-07
    97/100 stars
      Buy from Supplier

    Image Search Results