m na3 vo4  (Roche)


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    Structured Review

    Roche m na3 vo4
    M Na3 Vo4, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Roche
    Average 91 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-09
    91/100 stars

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    Related Articles

    Transfection:

    Article Title: Eukaryotic Elongation Factor 1A Interacts with Sphingosine Kinase and Directly Enhances Its Catalytic Activity *
    Article Snippet: .. The cells were transiently transfected using the calcium phosphate precipitation method , harvested 24 h later by scraping into cold PBS, and lysed by sonication (3 watts for 30 s at 4 °C) in extraction buffer containing 50 m m Tris/HCl, pH 7.4, 150 m m NaCl, 2 m m Na3 VO4 , 10m m NaF, 1 m m EDTA, 10% glycerol, 0.05% Triton X-100, 10 m m β-glycerophosphate, 1 m m dithiothreitol, and protease inhibitors (Complete™; Roche Applied Science). .. Protein concentrations of cell lysates were determined with Coomassie Brilliant Blue reagent (Sigma) using bovine serum albumin as standard.

    Article Title: The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation *The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation * S⃞
    Article Snippet: .. GST Pulldowns —HEK293FT cells were transfected with GFP-tagged full-length LRRK2 or Myc-tagged truncated LRRK2 and lysed in 20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, protease inhibitor mixture (Roche Applied Science). .. Glutathione-Sepharose beads with purified recombinant proteins were incubated with total cell lysates for 2 h at 4 °C.

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Protease Inhibitor:

    Article Title: Islet proteomics reveals genetic variation in dopamine production resulting in altered insulin secretion
    Article Snippet: .. After preincubation, 15 islets were transferred to an Eppendorf tube containing 125 μl of secretion media (KRB with 0.5% BSA and 3.3 m m glucose or 16.7 m m glucose with or without 1 μ m dopamine) and placed in a 37 °C water bath for 45 min. After the secretion, the secretion media were removed from the islets, and the islets were lysed in Nonidet P-40 lysis buffer (100 m m Tris (pH 8.0), 300 m m NaCl, 10 m m NaF, 2 m m Na3 VO4 , 2% Nonidet P-40 alternative, cOmplete mini EDTA-free protease inhibitor mixture (Roche Applied Science)). .. A.), R01 DK046960 (to R. T. K.), and P41 GM108538 and R35 GM118110 (to J. J. C.) and the Clinical and Translational Science Award (CTSA) program through National Institutes of Health National Center for Advancing Translational Sciences (NCATS) Grant UL1TR000427.

    Article Title: The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation *The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation * S⃞
    Article Snippet: .. GST Pulldowns —HEK293FT cells were transfected with GFP-tagged full-length LRRK2 or Myc-tagged truncated LRRK2 and lysed in 20 m m Tris-HCl, pH 7.5, 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, protease inhibitor mixture (Roche Applied Science). .. Glutathione-Sepharose beads with purified recombinant proteins were incubated with total cell lysates for 2 h at 4 °C.

    Article Title: Complement 1q-like-3 protein inhibits insulin secretion from pancreatic β-cells via the cell adhesion G protein–coupled receptor BAI3
    Article Snippet: .. Cells were lysed in 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 m m phenylmethylsulfonyl fluoride, and protease inhibitor mixture tablet (Roche Applied Science). .. The extraction of soluble proteins, quantitation, and Western blot analysis was performed as described previously ( ).

    Article Title: Glutamate Stimulates Oligodendrocyte Progenitor Migration Mediated via an αv Integrin/Myelin Proteolipid Protein Complex
    Article Snippet: .. Cell lysates were made using buffer A: 0.15 m NaCl, 0.05 m Tris, 0.5 m m EDTA, 1% Triton X-100, 0.05% SDS, 5 m m NaF, and 1 m m Na3 VO4 , pH 7.5, supplemented with Complete Mini Protease Inhibitor Cocktail (Roche Applied Science, Indianapolis, IN). .. Protein concentrations were determined by the bicinchoninic acid method (Sigma).

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Article Title: Expression of murine muscle-enriched A-type lamin-interacting protein (MLIP) is regulated by tissue-specific alternative transcription start sites
    Article Snippet: .. Proteins were extracted using lysis buffer containing 50 m m Tris-HCl (pH 8.0), 200 m m NaCl, 20 m m NaF, 20 m m β-glycerol phosphate, 0.5% Nonidet P-40, 0.1 m m Na3 Vo4 , 1 m m DTT, 1× protease inhibitor mixture (Roche Applied Science; one tablet/7.0 ml), and phosphatase inhibitor mixtures (Sigma; 0.1 ml/7.0 ml). ..

    Cell Culture:

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Immunoprecipitation:

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Expressing:

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Sonication:

    Article Title: Eukaryotic Elongation Factor 1A Interacts with Sphingosine Kinase and Directly Enhances Its Catalytic Activity *
    Article Snippet: .. The cells were transiently transfected using the calcium phosphate precipitation method , harvested 24 h later by scraping into cold PBS, and lysed by sonication (3 watts for 30 s at 4 °C) in extraction buffer containing 50 m m Tris/HCl, pH 7.4, 150 m m NaCl, 2 m m Na3 VO4 , 10m m NaF, 1 m m EDTA, 10% glycerol, 0.05% Triton X-100, 10 m m β-glycerophosphate, 1 m m dithiothreitol, and protease inhibitors (Complete™; Roche Applied Science). .. Protein concentrations of cell lysates were determined with Coomassie Brilliant Blue reagent (Sigma) using bovine serum albumin as standard.

    Western Blot:

    Article Title: RACK1, a New ADAM12 Interacting Protein
    Article Snippet: .. Immunoprecipitation and Western Blotting —Hepatic stellate cells were transfected with the indicated expression vectors or siRNAs by the AMAXA method and cultured for 36 h. After one night in serum-starved medium, cells were treated for 15 min with 0.1 μ m PMA and then lysed at 4 °C in RIPA buffer containing 50 m m Tris-HCl, pH 7.4, 1% Triton X-100, 25 m m HEPES, 150 m m NaCl, 0.2% deoxycholate, 5 m m MgCl2 , 1 m m Na3 VO4 , 1 m m sodium fluoride, and a protease inhibitor mixture (Roche Applied Science). ..

    Lysis:

    Article Title: Islet proteomics reveals genetic variation in dopamine production resulting in altered insulin secretion
    Article Snippet: .. After preincubation, 15 islets were transferred to an Eppendorf tube containing 125 μl of secretion media (KRB with 0.5% BSA and 3.3 m m glucose or 16.7 m m glucose with or without 1 μ m dopamine) and placed in a 37 °C water bath for 45 min. After the secretion, the secretion media were removed from the islets, and the islets were lysed in Nonidet P-40 lysis buffer (100 m m Tris (pH 8.0), 300 m m NaCl, 10 m m NaF, 2 m m Na3 VO4 , 2% Nonidet P-40 alternative, cOmplete mini EDTA-free protease inhibitor mixture (Roche Applied Science)). .. A.), R01 DK046960 (to R. T. K.), and P41 GM108538 and R35 GM118110 (to J. J. C.) and the Clinical and Translational Science Award (CTSA) program through National Institutes of Health National Center for Advancing Translational Sciences (NCATS) Grant UL1TR000427.

    Article Title: Rad9 Protein Contributes to Prostate Tumor Progression by Promoting Cell Migration and Anoikis Resistance *
    Article Snippet: .. Cells were lysed in radioimmune precipitation assay buffer (100 m m HEPES (pH 7.4), 10% glycerol, 150 m m NaCl, 1% Nonidet P-40, 0.1% SDS, 0.5% sodium deoxycholate, 30 m m NaF, 25 m m β-glycerophosphate, 1 m m Na3 VO4 , 2 m m EDTA, and complete protease inhibitors (Roche)) or in 1% Triton X-100 lysis buffer (100 m m HEPES (pH 7.4), 10% glycerol, 150 m m NaCl, 1% Triton X-100, 30 m m NaF, 25 m m β-glycerophosphate, 1 m m Na3 VO4 , 2 m m EDTA, and complete protease inhibitors (Roche)) for 30 min on ice. .. Protein was subjected to either 8% Tris-glycine or 4–12% Bis/Tris (Invitrogen) SDS-PAGE.

    Article Title: Expression of murine muscle-enriched A-type lamin-interacting protein (MLIP) is regulated by tissue-specific alternative transcription start sites
    Article Snippet: .. Proteins were extracted using lysis buffer containing 50 m m Tris-HCl (pH 8.0), 200 m m NaCl, 20 m m NaF, 20 m m β-glycerol phosphate, 0.5% Nonidet P-40, 0.1 m m Na3 Vo4 , 1 m m DTT, 1× protease inhibitor mixture (Roche Applied Science; one tablet/7.0 ml), and phosphatase inhibitor mixtures (Sigma; 0.1 ml/7.0 ml). ..

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  • 91
    Roche m na3 vo4
    M Na3 Vo4, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Roche
    Average 91 stars, based on 76 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    94
    Roche buffer
    Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 730 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/buffer/product/Roche
    Average 94 stars, based on 730 article reviews
    Price from $9.99 to $1999.99
    buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    94
    Roche nonidet p 40 lysis buffer
    Nonidet P 40 Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nonidet p 40 lysis buffer/product/Roche
    Average 94 stars, based on 161 article reviews
    Price from $9.99 to $1999.99
    nonidet p 40 lysis buffer - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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