Structured Review

FUJIFILM m na3 vo4
M Na3 Vo4, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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m na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Immunoprecipitation:

Article Title: Sirtuin1 Maintains Actin Cytoskeleton by Deacetylation of Cortactin in Injured Podocytes
Article Snippet: .. For immunoprecipitation, the cells were lysed by Triton cell lysis buffer containing 1 M Tris-buffer (pH 7.4), 100 mM NaCl, 5 mM EDTA, 10 mM sodium pyrophosphate (Sigma-Aldrich), 200 μ M Na3 VO4 (Wako Pure Chemical), and 1% Triton X-100; 1 ml cell extract containing 300–400 μ g protein was incubated with 5–10 μ g target antibody or control IgG overnight at 4°C and subsequently incubated with 30 μ g protein G sepharose (GE Healthcare) for 2 hours at 4°C. .. Proteins bound to the beads were eluted by boiling at 95°C for 5 minutes, and the extract solution was used for WB analysis.

Incubation:

Article Title: Sirtuin1 Maintains Actin Cytoskeleton by Deacetylation of Cortactin in Injured Podocytes
Article Snippet: .. For immunoprecipitation, the cells were lysed by Triton cell lysis buffer containing 1 M Tris-buffer (pH 7.4), 100 mM NaCl, 5 mM EDTA, 10 mM sodium pyrophosphate (Sigma-Aldrich), 200 μ M Na3 VO4 (Wako Pure Chemical), and 1% Triton X-100; 1 ml cell extract containing 300–400 μ g protein was incubated with 5–10 μ g target antibody or control IgG overnight at 4°C and subsequently incubated with 30 μ g protein G sepharose (GE Healthcare) for 2 hours at 4°C. .. Proteins bound to the beads were eluted by boiling at 95°C for 5 minutes, and the extract solution was used for WB analysis.

Cell Culture:

Article Title: Dynein Dysfunction Induces Endocytic Pathology Accompanied by an Increase in Rab GTPases
Article Snippet: .. Neuro2a cells were cultured in serum-free culture medium containing 1% insulin-transferrin-selenium X supplement (Invitrogen) and then treated with okadaic acid (OA; 1 and 5 n m ) (Calbiochem) dissolved in DMSO for 24 or 48 h. To wash and harvest cells treated with OA, we used PBS containing 10 m m NaF (Wako) and 2 m m Na3 VO4 (Wako) to avoid dephosphorylation. ..

De-Phosphorylation Assay:

Article Title: Dynein Dysfunction Induces Endocytic Pathology Accompanied by an Increase in Rab GTPases
Article Snippet: .. Neuro2a cells were cultured in serum-free culture medium containing 1% insulin-transferrin-selenium X supplement (Invitrogen) and then treated with okadaic acid (OA; 1 and 5 n m ) (Calbiochem) dissolved in DMSO for 24 or 48 h. To wash and harvest cells treated with OA, we used PBS containing 10 m m NaF (Wako) and 2 m m Na3 VO4 (Wako) to avoid dephosphorylation. ..

Lysis:

Article Title: Sirtuin1 Maintains Actin Cytoskeleton by Deacetylation of Cortactin in Injured Podocytes
Article Snippet: .. For immunoprecipitation, the cells were lysed by Triton cell lysis buffer containing 1 M Tris-buffer (pH 7.4), 100 mM NaCl, 5 mM EDTA, 10 mM sodium pyrophosphate (Sigma-Aldrich), 200 μ M Na3 VO4 (Wako Pure Chemical), and 1% Triton X-100; 1 ml cell extract containing 300–400 μ g protein was incubated with 5–10 μ g target antibody or control IgG overnight at 4°C and subsequently incubated with 30 μ g protein G sepharose (GE Healthcare) for 2 hours at 4°C. .. Proteins bound to the beads were eluted by boiling at 95°C for 5 minutes, and the extract solution was used for WB analysis.