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Cell Signaling Technology Inc m na3 vo4
M Na3 Vo4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m na3 vo4/product/Cell Signaling Technology Inc
Average 92 stars, based on 3 article reviews
Price from $9.99 to $1999.99
m na3 vo4 - by Bioz Stars, 2020-07
92/100 stars

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Lysis:

Article Title: Disruption of Nongenomic Testosterone Signaling in a Model of Spinal and Bulbar Muscular Atrophy
Article Snippet: .. Cell lysates were prepared in lysis buffer [20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m EGTA, 1 m m EDTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin; Cell Signaling] supplemented with protease inhibitors (Mini Complete; Roche, France). .. Lysates were centrifuged at 3000 × g for 10 min at 4 C, and protein concentrations of supernatants were determined by the BCA (bicinchoninic acid) method (Thermo Fisher Scientific, France).

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

Article Title: Phloretin Inhibits Phorbol Ester-Induced Tumor Promotion and Expression of Cyclooxygenase-2 in Mouse Skin: Extracellular Signal-Regulated Kinase and Nuclear Factor-?B as Potential Targets
Article Snippet: .. Collected tissues were lysed in 600 μL of lysis buffer per sample (20 m M Tris-HCl [pH 7.4], 150 m M NaCl, 1 m M EDTA, 1 m M EGTA, 1% Triton X-100, 2.5 m M sodium pyrophosphate, 1 m M glycerophosphate, 1 m M Na3 VO4 , and 1 g/mL leupeptin). .. The lysates were centrifuged, and the supernatant was incubated with specific immobilized pERK monoclonal antibodies with gentle rocking overnight at 4°C.

Article Title: NADPH:Quinone Oxidoreductase 1 Regulates Host Susceptibility to Ozone via Isoprostane Generation *
Article Snippet: .. Beads were washed twice with lysis buffer and then twice with kinase reaction buffer (25 m m Tris-HCl (pH 7.5), 5 m m β-glycerophosphate, 2 m m dithiothreitol (DTT), 0.1 m m Na3 VO4 , and 10 m m MgCl2 ) (Cell Signaling Technology). .. The IKK activity assay was initiated by the addition of kinase buffer containing 200 μ m ATP and 1 μg of GST-IκBα as a substrate for the IKK enzyme activity.

Article Title: Phloretin Inhibits Phorbol Ester-Induced Tumor Promotion and Expression of Cyclooxygenase-2 in Mouse Skin: Extracellular Signal-Regulated Kinase and Nuclear Factor-?B as Potential Targets
Article Snippet: .. The beads were washed twice each with 500 μL of lysis buffer and the same volume of kinase buffer (25 m M Tris-HCl [pH 7.5], 5 m M glycerophosphate, 2 m M dithiothreitol, 0.1 m M Na3 VO4 , and 10 m M MgCl2 ). ..

Article Title: A Role for cGMP in Inducible Nitric-oxide Synthase (iNOS)-induced Tumor Necrosis Factor (TNF) ?-converting Enzyme (TACE/ADAM17) Activation, Translocation, and TNF Receptor 1 (TNFR1) Shedding in Hepatocytes *
Article Snippet: .. Treated hepatocytes were washed twice in PBS and lysed with 1× cell lysis buffer (Cell Signaling Technology) containing 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerol phosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, and 1 μg/ml phenylmethylsulfonyl fluoride (PMSF) on ice for 10 min. Liver samples were homogenized with a glass Dounce homogenizer in 1× radioimmune precipitation assay buffer (cell lysis buffer; Cell Signaling Technology) containing 20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m Na2 EDTA, 1 m m EGTA, 1% Nonidet P-40, 1% sodium deoxycholate, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 V04 , 1 μg/ml leupeptin, and 1 m m PMSF in double distilled H2 O. .. The protein content of cell lysates was determined by BCA protein assay (Pierce).

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

Article Title: NADPH:Quinone Oxidoreductase 1 Regulates Host Susceptibility to Ozone via Isoprostane Generation *
Article Snippet: .. Ozone-exposed cells were rinsed with ice-cold PBS containing 1 m m phenylmethylsulfonyl fluoride (PMSF) and lysed for 5 min on ice in Cell Lysis Buffer (20 m m Tris (pH 7.5), 150 m m NaCl, 1 m m EDTA, 1 m m EGTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m glycerol phosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin, and 1 m m phenylmethylsulfonyl fluoride) (Cell Signaling Technology, Beverly, MA) containing both 1× Complete protease (Roche Applied Science) and 1× phosphatase inhibitor mixtures (Sigma). ..

Incubation:

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

Protease Inhibitor:

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

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    Cell Signaling Technology Inc m na3 vo4
    M Na3 Vo4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m na3 vo4/product/Cell Signaling Technology Inc
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m na3 vo4 - by Bioz Stars, 2020-07
    92/100 stars
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