random primed cdna  (New England Biolabs)


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    Name:
    M MuLV Reverse Transcriptase
    Description:
    M MuLV Reverse Transcriptase 50 000 units
    Catalog Number:
    m0253l
    Price:
    288
    Size:
    50 000 units
    Category:
    Reverse Transcriptases
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    Structured Review

    New England Biolabs random primed cdna
    M MuLV Reverse Transcriptase
    M MuLV Reverse Transcriptase 50 000 units
    https://www.bioz.com/result/random primed cdna/product/New England Biolabs
    Average 91 stars, based on 2724 article reviews
    Price from $9.99 to $1999.99
    random primed cdna - by Bioz Stars, 2020-09
    91/100 stars

    Images

    1) Product Images from "Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity"

    Article Title: Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

    Journal:

    doi: 10.1101/gad.1400506

    Ligand 15 inhibits prednisolone-regulated cell proliferation and gene expression in A549 cells. ( A ) A549 cells were treated as indicated for 4–5 h. Total RNA was isolated and subjected to cDNA synthesis. The cDNA was then analyzed by qPCR to measure
    Figure Legend Snippet: Ligand 15 inhibits prednisolone-regulated cell proliferation and gene expression in A549 cells. ( A ) A549 cells were treated as indicated for 4–5 h. Total RNA was isolated and subjected to cDNA synthesis. The cDNA was then analyzed by qPCR to measure

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction

    2) Product Images from "Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity"

    Article Title: Novel arylpyrazole compounds selectively modulate glucocorticoid receptor regulatory activity

    Journal:

    doi: 10.1101/gad.1400506

    Ligand 15 inhibits prednisolone-regulated cell proliferation and gene expression in A549 cells. ( A ) A549 cells were treated as indicated for 4–5 h. Total RNA was isolated and subjected to cDNA synthesis. The cDNA was then analyzed by qPCR to measure
    Figure Legend Snippet: Ligand 15 inhibits prednisolone-regulated cell proliferation and gene expression in A549 cells. ( A ) A549 cells were treated as indicated for 4–5 h. Total RNA was isolated and subjected to cDNA synthesis. The cDNA was then analyzed by qPCR to measure

    Techniques Used: Expressing, Isolation, Real-time Polymerase Chain Reaction

    3) Product Images from "Characterization of Ascites-Derived Ovarian Tumor Cells from Spontaneously Occurring Ovarian Tumors of the Chicken: Evidence for E-Cadherin Upregulation"

    Article Title: Characterization of Ascites-Derived Ovarian Tumor Cells from Spontaneously Occurring Ovarian Tumors of the Chicken: Evidence for E-Cadherin Upregulation

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0057582

    Quantification of vimentin, N-cadherin, cytokeratin, ZEB1, and VEGF mRNA in chicken ovarian cancer (COVCAR) cells. Vimentin mRNA (A), N-cadherin mRNA (B), cytokeratin mRNA (C), ZEB1 mRNA (D), and VEGF mRNA (E) abundance in normal ovarian surface epithelial cells (N; n = 5 animals) and COVCAR cell lines (C5, C6, C7, C11, C19). Total RNA was extracted from cultured cells in passages 3–4 and treated with deoxyribonuclease-I. Following reverse transcription, approximately 50 ng of cDNA was used in quantitative real-time PCR using SYBR® green as the dye to quantify vimentin mRNA, N-cadherin mRNA, cytokeratin mRNA, ZEB1 mRNA, VEGF mRNA, or β-actin mRNA in separate reactions. Each reaction was run in triplicate per cell line and the critical threshold ( C T ) values were subtracted from that of β-actin mRNA, averaged and converted from log-linear to linear term. * P
    Figure Legend Snippet: Quantification of vimentin, N-cadherin, cytokeratin, ZEB1, and VEGF mRNA in chicken ovarian cancer (COVCAR) cells. Vimentin mRNA (A), N-cadherin mRNA (B), cytokeratin mRNA (C), ZEB1 mRNA (D), and VEGF mRNA (E) abundance in normal ovarian surface epithelial cells (N; n = 5 animals) and COVCAR cell lines (C5, C6, C7, C11, C19). Total RNA was extracted from cultured cells in passages 3–4 and treated with deoxyribonuclease-I. Following reverse transcription, approximately 50 ng of cDNA was used in quantitative real-time PCR using SYBR® green as the dye to quantify vimentin mRNA, N-cadherin mRNA, cytokeratin mRNA, ZEB1 mRNA, VEGF mRNA, or β-actin mRNA in separate reactions. Each reaction was run in triplicate per cell line and the critical threshold ( C T ) values were subtracted from that of β-actin mRNA, averaged and converted from log-linear to linear term. * P

    Techniques Used: Cell Culture, Real-time Polymerase Chain Reaction, SYBR Green Assay

    Expression of genes in chicken ovarian cancer cells (COVCAR) and normal ovarian surface epithelial cells (NOSE). RT-PCR analyses for expression of various cytoskeletal proteins, growth factors and receptors, protein/enzymes related to steroid hormone synthesis, gonadal hormone and hormone receptors in chicken ovarian cancer cell lines (C5, C6, C7, C11, C19) and normal ovarian surface epithelial cells (NOSE; n = 5 animals). Total RNA was extracted from cultured cells in passages 3–4 and treated with deoxyribonuclease-I. Approximately 250 ng of cDNA (+RT) was used as template to amplify the gene products. Contamination controls consisted of reverse transcribed RNA without reverse transcriptase (-RT). M- DNA size marker.
    Figure Legend Snippet: Expression of genes in chicken ovarian cancer cells (COVCAR) and normal ovarian surface epithelial cells (NOSE). RT-PCR analyses for expression of various cytoskeletal proteins, growth factors and receptors, protein/enzymes related to steroid hormone synthesis, gonadal hormone and hormone receptors in chicken ovarian cancer cell lines (C5, C6, C7, C11, C19) and normal ovarian surface epithelial cells (NOSE; n = 5 animals). Total RNA was extracted from cultured cells in passages 3–4 and treated with deoxyribonuclease-I. Approximately 250 ng of cDNA (+RT) was used as template to amplify the gene products. Contamination controls consisted of reverse transcribed RNA without reverse transcriptase (-RT). M- DNA size marker.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Marker

    Related Articles

    Mutagenesis:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB). .. RNA was combined with primers and annealed in water at 70°C for 5 minutes followed by incubation on ice.

    Produced:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. The length distribution of the 3173 Pol cDNAs was visibly shorter than that produced by the MMLV RT, although a subset of the 3173 extension products appeared to be so large that they barely entered the gel. .. Incubation of the DNA primer:RNA template complex with the Taq Pol negative control resulted in a structure-dependent 5′-3′ exonuclease cleavage product that migrated at the dye front .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Two-step RT-PCR reactions were performed using either 5 units 3173 Pol, exonuclease negative mutant or 200 Units MMLV RT (NEB). .. RNA was combined with primers and annealed in water at 70°C for 5 minutes followed by incubation on ice.

    other:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: The 3173 Pol and MMLV RT were both able to extend the primer to produce faint, nearly full-length products although the 3173 Pol product was detectably longer than that of MMLV RT.

    Activity Assay:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. As an additional test to compare the RT activity of the 3173 Pol to that of MMLV-RT on a complex RNA substrate, a primer specific to bases 714 to 690 of the negative sense RNA MS2 genome was 5′-fluorophore-labeled. .. The labeled cDNA primer was extended using extracted MS2 RNA as a template ( ).

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Extension products from a 5′-fluorophore-labeled dT20 primer were resolved by denaturing polyacrylamide gel to further demonstrate RT activity and to assess the relative lengths of the extension products of the 3173 Pol and MMLV RT ( ). ..

    Sequencing:

    Article Title: Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
    Article Snippet: .. Of the two longer target sequences tested, only the 821 bp beta-actin sequence (Lane C3) was reverse transcribed by the 3173 Pol and this synthesis appeared less efficient than that of MMLV RT. ..

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