m mlv rtase  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 91

    Structured Review

    Thermo Fisher m mlv rtase
    M Mlv Rtase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv rtase/product/Thermo Fisher
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m mlv rtase - by Bioz Stars, 2020-07
    91/100 stars

    Images

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish
    Article Snippet: .. The mixture was then immediately chilled on ice for 5 min. deoxynucleotide triphosphate (dNTP) mixture, dithiothreitol (DTT), and RNase inhibitor were add and incubated at 37°C for 2 min. M-MLV RTase was then added and the RT reaction was carried out. qPCR was performed using SYBR® Green PCR Master Mix (Invitrogen) using an ABI 7300 real-time PCR system following the manufacturer's instructions. .. Data analysis was performed with SDS 2.3 software (Applied Biosystems).

    RNA Extraction:

    Article Title: The lens-regenerating competence in the outer cornea and epidermis of larval Xenopus laevis is related to pax6 expression
    Article Snippet: .. For total RNA extraction, tissue pools were processed according to the ‘SV Total RNA Isolation System’ (Promega) and reverse-transcribed using M-MLV RTase (Gibco BRL). ..

    Amplification:

    Article Title: Cryptotanshinone exhibits therapeutical effects on cerebral stroke through the PI3K/AKT-eNOS signaling pathway
    Article Snippet: .. A reverse transcription kit (Fermentas; Thermo Fisher Scientific, Inc.) was used to synthesis cDNA through a total volume of 25 µl (12 µl RNA-primer Mix, 5 µl 5X RT reaction buffer, 1 µl 25 mM dNTPs, 1 µl 25 U/µl RNase inhibitor, 1 µl 200 U/µl M-MLV Rtase, 1 µl Oligo(dt)18 and 4 µl DNase-free ddH2 O). cDNA was then amplified using a SYBR Green PCR kit (Thermo Fisher Scientific, Inc.) through a total PCR system of 25 µl (12.5 µl SYBR Green Mix, 0.5 µl forward primer, 0.5 µl reverse primer, 9.5 µl ddH2 O and 2 µl cDNA template). ..

    Synthesized:

    Article Title: Transcriptome differences between Cry1Ab resistant and susceptible strains of Asian corn borer
    Article Snippet: .. First-strand cDNA was synthesized from 4 ug of DNA-free RNA using the M-MLV Rtase (Thermo, USA) according to the manufacture’s instructions. .. The cDNA was used as the template for RT-qPCR.

    Isolation:

    Article Title: The lens-regenerating competence in the outer cornea and epidermis of larval Xenopus laevis is related to pax6 expression
    Article Snippet: .. For total RNA extraction, tissue pools were processed according to the ‘SV Total RNA Isolation System’ (Promega) and reverse-transcribed using M-MLV RTase (Gibco BRL). ..

    SYBR Green Assay:

    Article Title: U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish
    Article Snippet: .. The mixture was then immediately chilled on ice for 5 min. deoxynucleotide triphosphate (dNTP) mixture, dithiothreitol (DTT), and RNase inhibitor were add and incubated at 37°C for 2 min. M-MLV RTase was then added and the RT reaction was carried out. qPCR was performed using SYBR® Green PCR Master Mix (Invitrogen) using an ABI 7300 real-time PCR system following the manufacturer's instructions. .. Data analysis was performed with SDS 2.3 software (Applied Biosystems).

    Article Title: Cryptotanshinone exhibits therapeutical effects on cerebral stroke through the PI3K/AKT-eNOS signaling pathway
    Article Snippet: .. A reverse transcription kit (Fermentas; Thermo Fisher Scientific, Inc.) was used to synthesis cDNA through a total volume of 25 µl (12 µl RNA-primer Mix, 5 µl 5X RT reaction buffer, 1 µl 25 mM dNTPs, 1 µl 25 U/µl RNase inhibitor, 1 µl 200 U/µl M-MLV Rtase, 1 µl Oligo(dt)18 and 4 µl DNase-free ddH2 O). cDNA was then amplified using a SYBR Green PCR kit (Thermo Fisher Scientific, Inc.) through a total PCR system of 25 µl (12.5 µl SYBR Green Mix, 0.5 µl forward primer, 0.5 µl reverse primer, 9.5 µl ddH2 O and 2 µl cDNA template). ..

    Incubation:

    Article Title: U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish
    Article Snippet: .. The mixture was then immediately chilled on ice for 5 min. deoxynucleotide triphosphate (dNTP) mixture, dithiothreitol (DTT), and RNase inhibitor were add and incubated at 37°C for 2 min. M-MLV RTase was then added and the RT reaction was carried out. qPCR was performed using SYBR® Green PCR Master Mix (Invitrogen) using an ABI 7300 real-time PCR system following the manufacturer's instructions. .. Data analysis was performed with SDS 2.3 software (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: U1 snRNP is mislocalized in ALS patient fibroblasts bearing NLS mutations in FUS and is required for motor neuron outgrowth in zebrafish
    Article Snippet: .. The mixture was then immediately chilled on ice for 5 min. deoxynucleotide triphosphate (dNTP) mixture, dithiothreitol (DTT), and RNase inhibitor were add and incubated at 37°C for 2 min. M-MLV RTase was then added and the RT reaction was carried out. qPCR was performed using SYBR® Green PCR Master Mix (Invitrogen) using an ABI 7300 real-time PCR system following the manufacturer's instructions. .. Data analysis was performed with SDS 2.3 software (Applied Biosystems).

    Article Title: Cryptotanshinone exhibits therapeutical effects on cerebral stroke through the PI3K/AKT-eNOS signaling pathway
    Article Snippet: .. A reverse transcription kit (Fermentas; Thermo Fisher Scientific, Inc.) was used to synthesis cDNA through a total volume of 25 µl (12 µl RNA-primer Mix, 5 µl 5X RT reaction buffer, 1 µl 25 mM dNTPs, 1 µl 25 U/µl RNase inhibitor, 1 µl 200 U/µl M-MLV Rtase, 1 µl Oligo(dt)18 and 4 µl DNase-free ddH2 O). cDNA was then amplified using a SYBR Green PCR kit (Thermo Fisher Scientific, Inc.) through a total PCR system of 25 µl (12.5 µl SYBR Green Mix, 0.5 µl forward primer, 0.5 µl reverse primer, 9.5 µl ddH2 O and 2 µl cDNA template). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher m mlv rt reagent kit
    M Mlv Rt Reagent Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv rt reagent kit/product/Thermo Fisher
    Average 99 stars, based on 70 article reviews
    Price from $9.99 to $1999.99
    m mlv rt reagent kit - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher m mlv rtase
    M Mlv Rtase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv rtase/product/Thermo Fisher
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m mlv rtase - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    Image Search Results