m mlv moloney murine leukemia virus derived reverse transcriptase  (TaKaRa)

 
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    Name:
    MMLV Reverse Transcriptase
    Description:
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    Catalog Number:
    639575
    Price:
    None
    Size:
    8 000 Units
    Category:
    MMLV RT GPR Reverse transcriptases cDNA synthesis
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    Structured Review

    TaKaRa m mlv moloney murine leukemia virus derived reverse transcriptase
    MMLV Reverse Transcriptase GPR is a registered general purpose reagent GPR appropriate for use in general laboratory applications including molecular diagnostic development and testing It is an ultra pure recombinant Moloney Murine Leukemia Virus reverse transcriptase MMLV RT ideal for a wide range of applications This highly purified protein is a fully active RT that is free of exogenous RNases and other nucleases thus preventing degradation of template RNA As a result MMLV Reverse Transcriptase GPR is able to synthesize a higher percentage of high quality full length cDNAs than standard RTs
    https://www.bioz.com/result/m mlv moloney murine leukemia virus derived reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m mlv moloney murine leukemia virus derived reverse transcriptase - by Bioz Stars, 2020-08
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    Amplification:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    Synthesized:

    Article Title: AIMP3 Deletion Induces Acute Radiation Syndrome-like Phenotype in Mice
    Article Snippet: .. For siRNA-mediated knock-down validation, cells were lyzed in Trizol (Gibco) and cDNA were synthesized from the 1 μg of total RNA using MMLV-RT (Clontech). .. Level of AIMP3, BRCA1, XRCC5 and GARS were measured by SyGr (Bio-rad) using AB7500 real time PCR.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Global profiling of alternative splicing landscape responsive to drought, heat and their combination in wheat (Triticum aestivum L.)
    Article Snippet: .. RT‐PCR validation of AS events Total RNA treated with DNase I were reverse‐transcribed with oligo‐dT primers using Reverse Transcriptase M‐MLV (TaKaRa, Kusatsu, Shiga, Japan) following the manufacturer's instructions. .. RT‐PCR was performed in a 10 μL reaction volume and primer pair for each gene was designed to amplify both two splice variants (isoforms 1 and 2) in a single reaction.

    Real-time Polymerase Chain Reaction:

    Article Title: ZIP4, a Novel Determinant of Tumor Invasion in Hepatocellular Carcinoma, Contributes to Tumor Recurrence after Liver Transplantation
    Article Snippet: .. Real-time polymerase chain reaction RNA was extracted using the TRIzol reagent (Invitrogen) and used for cDNA synthesis with a M-MLV Reverse Transcriptase (TaKaRa, Dalian, China). .. Quantitative real-time polymerase chain reaction (qRT-PCR) was conducted with SYBR Green PCR Master Mix (Applied Biosystems, Foster City, CA, USA) using the ABI Prism 7500 Real-time PCR System (Applied Biosystems).

    Polymerase Chain Reaction:

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

    cDNA Library Assay:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Sequencing:

    Article Title: Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin
    Article Snippet: .. Following mRNA elution from the Dynabeads, first strand cDNA synthesis was performed using MMLV reverse transcriptase (Clontech) using an anchored polyT primer tailed with a universal primer sequence (See for primer sequences and for pigment cell cDNA library construction overview.) .. A universal primer sequence was also added to the 3′ end of the first strand by template switching, allowing for PCR-amplification of the resultant cDNA , .

    Article Title: A Full Suite of Histone and Histone Modifying Genes Are Transcribed in the Dinoflagellate Lingulodinium
    Article Snippet: .. The H3 sequence was cloned by PCR using primers based on the transcriptome sequence (forward primer 5′-CATTACGCCTGACGCTGTCTACGTGC-3′ and reverse primer 5′- GTTAGCGTCTGCTGCTGACGGCTTC-3′ ) from a 1st strand cDNA sample prepared from Trizol (Invitrogen) extracted RNA using a reverse transcription reaction catalyzed by MMLV RT (Clontech) and the 5′ CDS primer A of the SMARTer RACE cDNA Amplification kit (Clontech). .. A second PCR, performed on the first PCR product using the forward primer 5′-TCAGTCggatccATGGCCCGCACGAAGCAG-3′ (containing a BamH1 site indicated by small letters) was used to allow directional cloning into the BamH1 and Sma1 restriction sites of the bacterial expression vector pQE30 (Qiagen).

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  • 99
    TaKaRa m mlv moloney murine leukemia virus derived reverse transcriptase
    M Mlv Moloney Murine Leukemia Virus Derived Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mlv moloney murine leukemia virus derived reverse transcriptase/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m mlv moloney murine leukemia virus derived reverse transcriptase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

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