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Millipore m lysine
M Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m lysine/product/Millipore
Average 88 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m lysine - by Bioz Stars, 2020-09
88/100 stars

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Cell Culture:

Article Title: Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36 *
Article Snippet: .. Suz12 KO mouse ESCs were cultured for 6 days in SILAC Dulbecco's modified Eagle's medium (Sigma) containing 15% dialyzed fetal bovine serum (Invitrogen), penicillin-streptomycin (Invitrogen), nonessential amino acids (Invitrogen), pyruvate (Invitrogen), 50 m m β-mercaptoethanol, 3.5 g/liter d -glucose, 107 units/ml ESGRO leukemia inhibitory factor (Chemicon), 0.802 m m l -leucine (Sigma), 0.398 m m l -arginine (Sigma), and 0.798 m m l -lysine (Sigma). .. Lys8 isotope (Cambridge Isotopes, CNLM-291) was used for the Suz12 KO ESCs.

Incubation:

Article Title: Reducing Intracortical Inhibition in the Adult Visual Cortex Promotes Ocular Dominance Plasticity
Article Snippet: .. Briefly, free-floating sections were treated with blocking solution composed of 3% BSA and 20 m m lysine in PBS, pH 7.4, for 1 h. Sections were then incubated overnight at 4°C with monoclonal antibody Cat-315 (1:300 in PBS; Millipore Bioscience Research Reagents) or in a solution of biotin-conjugated lectin Wisteria floribunda (WFA) (10 μg/ml; Sigma-Aldrich). .. Cat-315 was then revealed with biotinylated anti-mouse IgG (1:100; Vector Laboratories), followed by Cy3-conjugated extravidin (1:500 in PBS; Sigma-Aldrich), incubated for 2 and 1 h respectively.

Article Title: Endogenous TWEAK is critical for regulating the function of mouse uterine natural killer cells in an immunological model of pregnancy loss
Article Snippet: .. The sections were then incubated (2 hr at room temperature) with Cy3‐tagged streptavidin (1 : 75), treated with 20 m m l ‐lysine (Sigma) to quench the autofluorescence, and finally cover‐slipped with DAPI‐supplemented mounting media (ProLong Gold Anti‐fade Reagent with 4,6‐diamidino‐2‐phenylindole; Invitrogen). .. Photomicrographs were collected using an AxioCam equipped Zeiss M1 Imager with axiovision software (Carl Zeiss, Toronto, ON, Canada).

Article Title: Proteomic Screen for Cellular Targets of the Vaccinia Virus F10 Protein Kinase Reveals that Phosphorylation of mDia Regulates Stress Fiber Formation *
Article Snippet: .. FLP-In T-REx 293 c.o.-3xFLAG-F10 cells were incubated for 10 doublings in SILAC media (Sigma) supplemented with 10% FCS and either 0.398 m m l -arginine and 0.798 m m l -lysine (light media) (Sigma) or 0.398 m m 13 C6 , 15 N4 l -arginine and 0.798 m m 13 C6 , 15 N2 l -lysine (heavy media) (Sigma). ..

Article Title: Identification of Cardiac Myosin-binding Protein C as a Candidate Biomarker of Myocardial Infarction by Proteomics Analysis *
Article Snippet: .. After incubation on ice for 30 min in the dark, the labeling reaction was stopped by scavenging non-bound dye with 10 m m l -lysine (catalogue number L8662, Sigma) for 15 min. For two-dimensional gel electrophoresis, 20 μg of proteins from each group were mixed with the same volume of DIGE 2× buffer (8 m urea, 4% (w/v) CHAPS, 2% (w/v) DTT, and 2% (v/v) Pharmalytes pH 3–10 for IEF), and 20 μg per sample were diluted in rehydration solution (8 m urea, 0.5% (w/v) CHAPS, 0.2% (w/v) DTT, and 0.2% (v/v) Pharmalytes pH 3–10) and loaded on IPG strips (18 cm, pH 3–10, nonlinear, GE Healthcare). .. After rehydration overnight, strips were focused at 60 kV-h at 20 °C (Multiphor II, GE Healthcare).

Labeling:

Article Title: Identification of Cardiac Myosin-binding Protein C as a Candidate Biomarker of Myocardial Infarction by Proteomics Analysis *
Article Snippet: .. After incubation on ice for 30 min in the dark, the labeling reaction was stopped by scavenging non-bound dye with 10 m m l -lysine (catalogue number L8662, Sigma) for 15 min. For two-dimensional gel electrophoresis, 20 μg of proteins from each group were mixed with the same volume of DIGE 2× buffer (8 m urea, 4% (w/v) CHAPS, 2% (w/v) DTT, and 2% (v/v) Pharmalytes pH 3–10 for IEF), and 20 μg per sample were diluted in rehydration solution (8 m urea, 0.5% (w/v) CHAPS, 0.2% (w/v) DTT, and 0.2% (v/v) Pharmalytes pH 3–10) and loaded on IPG strips (18 cm, pH 3–10, nonlinear, GE Healthcare). .. After rehydration overnight, strips were focused at 60 kV-h at 20 °C (Multiphor II, GE Healthcare).

Two-Dimensional Gel Electrophoresis:

Article Title: Identification of Cardiac Myosin-binding Protein C as a Candidate Biomarker of Myocardial Infarction by Proteomics Analysis *
Article Snippet: .. After incubation on ice for 30 min in the dark, the labeling reaction was stopped by scavenging non-bound dye with 10 m m l -lysine (catalogue number L8662, Sigma) for 15 min. For two-dimensional gel electrophoresis, 20 μg of proteins from each group were mixed with the same volume of DIGE 2× buffer (8 m urea, 4% (w/v) CHAPS, 2% (w/v) DTT, and 2% (v/v) Pharmalytes pH 3–10 for IEF), and 20 μg per sample were diluted in rehydration solution (8 m urea, 0.5% (w/v) CHAPS, 0.2% (w/v) DTT, and 0.2% (v/v) Pharmalytes pH 3–10) and loaded on IPG strips (18 cm, pH 3–10, nonlinear, GE Healthcare). .. After rehydration overnight, strips were focused at 60 kV-h at 20 °C (Multiphor II, GE Healthcare).

Modification:

Article Title: Quantitative Mass Spectrometry of Histones H3.2 and H3.3 in Suz12-deficient Mouse Embryonic Stem Cells Reveals Distinct, Dynamic Post-translational Modifications at Lys-27 and Lys-36 *
Article Snippet: .. Suz12 KO mouse ESCs were cultured for 6 days in SILAC Dulbecco's modified Eagle's medium (Sigma) containing 15% dialyzed fetal bovine serum (Invitrogen), penicillin-streptomycin (Invitrogen), nonessential amino acids (Invitrogen), pyruvate (Invitrogen), 50 m m β-mercaptoethanol, 3.5 g/liter d -glucose, 107 units/ml ESGRO leukemia inhibitory factor (Chemicon), 0.802 m m l -leucine (Sigma), 0.398 m m l -arginine (Sigma), and 0.798 m m l -lysine (Sigma). .. Lys8 isotope (Cambridge Isotopes, CNLM-291) was used for the Suz12 KO ESCs.

Chloramphenicol Acetyltransferase Assay:

Article Title: Reducing Intracortical Inhibition in the Adult Visual Cortex Promotes Ocular Dominance Plasticity
Article Snippet: .. Briefly, free-floating sections were treated with blocking solution composed of 3% BSA and 20 m m lysine in PBS, pH 7.4, for 1 h. Sections were then incubated overnight at 4°C with monoclonal antibody Cat-315 (1:300 in PBS; Millipore Bioscience Research Reagents) or in a solution of biotin-conjugated lectin Wisteria floribunda (WFA) (10 μg/ml; Sigma-Aldrich). .. Cat-315 was then revealed with biotinylated anti-mouse IgG (1:100; Vector Laboratories), followed by Cy3-conjugated extravidin (1:500 in PBS; Sigma-Aldrich), incubated for 2 and 1 h respectively.

Electrofocusing:

Article Title: Identification of Cardiac Myosin-binding Protein C as a Candidate Biomarker of Myocardial Infarction by Proteomics Analysis *
Article Snippet: .. After incubation on ice for 30 min in the dark, the labeling reaction was stopped by scavenging non-bound dye with 10 m m l -lysine (catalogue number L8662, Sigma) for 15 min. For two-dimensional gel electrophoresis, 20 μg of proteins from each group were mixed with the same volume of DIGE 2× buffer (8 m urea, 4% (w/v) CHAPS, 2% (w/v) DTT, and 2% (v/v) Pharmalytes pH 3–10 for IEF), and 20 μg per sample were diluted in rehydration solution (8 m urea, 0.5% (w/v) CHAPS, 0.2% (w/v) DTT, and 0.2% (v/v) Pharmalytes pH 3–10) and loaded on IPG strips (18 cm, pH 3–10, nonlinear, GE Healthcare). .. After rehydration overnight, strips were focused at 60 kV-h at 20 °C (Multiphor II, GE Healthcare).

Blocking Assay:

Article Title: Reducing Intracortical Inhibition in the Adult Visual Cortex Promotes Ocular Dominance Plasticity
Article Snippet: .. Briefly, free-floating sections were treated with blocking solution composed of 3% BSA and 20 m m lysine in PBS, pH 7.4, for 1 h. Sections were then incubated overnight at 4°C with monoclonal antibody Cat-315 (1:300 in PBS; Millipore Bioscience Research Reagents) or in a solution of biotin-conjugated lectin Wisteria floribunda (WFA) (10 μg/ml; Sigma-Aldrich). .. Cat-315 was then revealed with biotinylated anti-mouse IgG (1:100; Vector Laboratories), followed by Cy3-conjugated extravidin (1:500 in PBS; Sigma-Aldrich), incubated for 2 and 1 h respectively.

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  • 99
    Millipore nitric acid poly l lysine solution
    Nitric Acid Poly L Lysine Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nitric acid poly l lysine solution/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nitric acid poly l lysine solution - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    88
    Millipore m lysine
    M Lysine, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m lysine/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m lysine - by Bioz Stars, 2020-09
    88/100 stars
      Buy from Supplier

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