m l glutamine  (Thermo Fisher)


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    Name:
    L Glutamine
    Description:
    L Glutamine is an amino acid required for cell culture L glutamine participates in the formation of purine and pyrimidine nucleotides amino sugars glutathione L glutamate other amino acids and is used in protein synthesis and glucose production L glutamine unlike most other amino acids is not stable in solution The rate at which this degradation proceeds is a function of time temperature and pH Using L glutamineL glutamine nonsterile powder should be made as a filter sterilized stock solution of 200 mM or similar in distilled water The optimal concentration to use is dependent upon the cell type and medium used to culture the cells but generally falls in the range of 2 6 mM Alternative productWe also offerGlutaMAX supplement as a stable alternative to L glutamine
    Catalog Number:
    21051024
    Price:
    None
    Category:
    Cell Culture Transfection Reagents
    Applications:
    Cell Culture|Mammalian Cell Culture
    Buy from Supplier


    Structured Review

    Thermo Fisher m l glutamine
    L Glutamine is an amino acid required for cell culture L glutamine participates in the formation of purine and pyrimidine nucleotides amino sugars glutathione L glutamate other amino acids and is used in protein synthesis and glucose production L glutamine unlike most other amino acids is not stable in solution The rate at which this degradation proceeds is a function of time temperature and pH Using L glutamineL glutamine nonsterile powder should be made as a filter sterilized stock solution of 200 mM or similar in distilled water The optimal concentration to use is dependent upon the cell type and medium used to culture the cells but generally falls in the range of 2 6 mM Alternative productWe also offerGlutaMAX supplement as a stable alternative to L glutamine
    https://www.bioz.com/result/m l glutamine/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m l glutamine - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    In Vivo:

    Article Title: Asymmetric functional contributions of acidic and aromatic side chains in sodium channel voltage-sensor domains
    Article Snippet: In particular, we set out to decipher potential similarities and differences between the VSDs of Kv and Nav channels, as well as putative functional diversity among individual Nav domains. .. Molecular biology and in vivo nonsense suppression Positions 151, 161, 168, 171, 197, 598, 621, 624, 646, 1051, 1069, 1076, 1079, 1373, 1396, 1399, or 1420 of rNav 1.4 (in pBASTA) were mutated by conventional site-directed mutagenesis to code for leucine (Leu), asparagine (Asn), aspartic acid (Asp), Gln, Glu, or a TAG (amber) stop codon (see Results for details). mRNA was transcribed using a mMessage mMachine T7 kit (Ambion) after linearization with NotI. .. Stage V–VI oocytes were harvested from Xenopus laevis frogs, injected, and incubated as described previously ( ).

    Mutagenesis:

    Article Title: Asymmetric functional contributions of acidic and aromatic side chains in sodium channel voltage-sensor domains
    Article Snippet: In particular, we set out to decipher potential similarities and differences between the VSDs of Kv and Nav channels, as well as putative functional diversity among individual Nav domains. .. Molecular biology and in vivo nonsense suppression Positions 151, 161, 168, 171, 197, 598, 621, 624, 646, 1051, 1069, 1076, 1079, 1373, 1396, 1399, or 1420 of rNav 1.4 (in pBASTA) were mutated by conventional site-directed mutagenesis to code for leucine (Leu), asparagine (Asn), aspartic acid (Asp), Gln, Glu, or a TAG (amber) stop codon (see Results for details). mRNA was transcribed using a mMessage mMachine T7 kit (Ambion) after linearization with NotI. .. Stage V–VI oocytes were harvested from Xenopus laevis frogs, injected, and incubated as described previously ( ).

    Modification:

    Article Title: ?-AP-2 Directs Myosin VI-dependent Endocytosis of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels in the Intestine *
    Article Snippet: .. The cells were grown in high glucose l -glutamine Dulbecco's modified Eagle's medium containing 10% fetal bovine serum (Invitrogen) and hygromycin B (150 μg/ml; Invitrogen) at 37 °C in 5% CO2 , 90% air atmosphere. ..

    Article Title: ?-AP-2 Directs Myosin VI-dependent Endocytosis of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channels in the Intestine *
    Article Snippet: The samples were then dehydrated in a graded series of ethanol, rinsed twice with propylene oxide, and incubated for 2 h each in 1:1, 3:1 and 1:0 (undiluted) mixtures of resin (EMbed 812; Electron Microscopy Science) and propylene oxide. .. CaCo-2BB e cells were obtained from ATCC and grown at 37 °C in 5% CO2 , 90% air atmosphere in high glucose with l -glutamine Dulbecco's modified Eagle's medium (Invitrogen), supplemented with 10% fetal bovine serum (Invitrogen), 10 μg/ml apo-transferrin (Chemicon International Temecula, CA), 1 m m /liter sodium pyruvate (Sigma), 1% penicillin-streptomycin (Invitrogen), 1 μg/ml Fungizone (Invitrogen), and 5 μg/ml Plasmocin (Invitrogen). .. The cells were seeded at 1 × 105 cells/cm2 on 100-mm cell culture dishes (Corning Incorporated, Corning, NY), and passaged at 70% confluency on a 75-mm Transwell filters (Costar Inc.).

    Cell Culture:

    Article Title: Infiltration and In-Tissue Polymerization of Photocross-Linked Hydrogel for Effective Fixation of Implants into Cartilage—An In Vitro Study
    Article Snippet: Then, we used fibrin glue (Tisseel, Baxter Healthcare Corp, Deerfield, IL) to fill the open space (without using PDLLA-PEG); these were the two controls. .. All cartilage explants were cultured in chondrogenic medium (DMEM with 1% l -alanyl-l -glutamine (GlutaMAX), 55 mg/L sodium pyruvate, 1% antibiotic–antimycotic, 1% insulin transferrin-selenium (Invitrogen, Carlsbad, CA, USA), 10 ng/mL transforming growth factor-β3 (TGF-β3; PeproTech, Rocky Hill, NJ, USA), 100 nM dexamethasone, 50 μM l -ascorbic acid 2-phosphate, and 23 μM l -proline). .. 4.3 Measuring the Weight of Polymers within the Cartilage After the photocross-linking, the entire construct was digested in papain solution (125 mg/mL papain, 50 mM sodium phosphate buffer, 2 mM N -acetyl cysteine (Sigma), pH 6.5) at 60 °C for 24 h. The weights of polymers from different groups were measured using an analytical balance (MS304S, Mettler Toledo, Switzerland).

    Article Title: Neuronatin regulates pancreatic β cell insulin content and secretion
    Article Snippet: Forty-eight hours later, transfected cells were lysed, clarified, normalized as above, and immunoprecipitated using anti–c-Myc agarose resin (Pierce) with Western blotting performed using antibodies against c-Myc (mouse monoclonal, Cell Signaling) and FLAG (mouse monoclonal, Sigma-Aldrich). .. For siRNA silencing, INS1E cells ( ) (cultured in RPMI, 10% FBS, 2 mM l -glutamine, 50 μM β-mercaptoethanol, 37°C, 5% CO2 ) were transfected with 50 nM of Silencer Select siRNAs ( Nnat , s137247; Sec11a , s80747; both Ambion) using Dharmafect 1 reagent (Dharmacon). ..

    Article Title: Neuronatin regulates pancreatic β cell insulin content and secretion
    Article Snippet: .. For siRNA silencing, INS1E cells ( ) (cultured in RPMI, 10% FBS, 2 mM l -glutamine, 50 μM β-mercaptoethanol, 37°C, 5% CO2 ) were transfected with 50 nM of Silencer Select siRNAs (Nnat , s137247; Sec11a , s80747; both Ambion) using Dharmafect 1 reagent (Dharmacon). ..

    Gas Chromatography:

    Article Title: Analysis of glutamine dependency in non-small cell lung cancer
    Article Snippet: NSCLC lines were seeded in a 96-well plate format in RPMI containing Glc and glutamax, and supplemented with 10% FBS at a growth rate-dependent density. .. After 24 h, media was carefully removed and cells were placed in 100ul RPMI containing Glc and Gln (Gibco 11875), or without Gln (Gibco 21700), or without Glc (Gibco 11879), and supplemented with 10% FBS. ..

    Transfection:

    Article Title: Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis
    Article Snippet: Per 24-well, the transfection mixture contained 0.5 μg promoter plasmid DNA (StAR, HSD3B2, CYP17A1 and CYP11A1) and 125 ng coregulator plasmid cDNA (e.g. RARB and Nur77) as well as 25 ng Renilla luciferase reporter (pRL-TK) (Promega, Maidson, WI) for endogenous control. .. Transfection medium [DMEM containing L-glutamine and 15 mm HEPES (GIBCO) supplemented with 10% NU-I serum (BD biosciences) and 0.1% selenium/insulin/transferrin (GIBCO)] was changed after 6 h and cells were cultivated in serum free medium for 24 h. Thereafter, cells were grown in the presence or absence of 1 μM ΑΤRA in serum-free medium for another 24 h. Finally, 48 hours after transfection, cells were washed with phosphate buffered saline (PBS) and then assayed for luciferase activity using the Dual Luciferase Reporter Assay system and the protocol by Promega. .. For overexpression studies of RARB, H295R cells were either transfected with the empty vector (pCMV5) or the vector expressing the cDNA of RARB in six wells (1.0 × 106 cells per well; Falcon 3046;BD biosciences) using Lipofectamine 2000 (Invitrogen).

    Article Title: Neuronatin regulates pancreatic β cell insulin content and secretion
    Article Snippet: Forty-eight hours later, transfected cells were lysed, clarified, normalized as above, and immunoprecipitated using anti–c-Myc agarose resin (Pierce) with Western blotting performed using antibodies against c-Myc (mouse monoclonal, Cell Signaling) and FLAG (mouse monoclonal, Sigma-Aldrich). .. For siRNA silencing, INS1E cells ( ) (cultured in RPMI, 10% FBS, 2 mM l -glutamine, 50 μM β-mercaptoethanol, 37°C, 5% CO2 ) were transfected with 50 nM of Silencer Select siRNAs ( Nnat , s137247; Sec11a , s80747; both Ambion) using Dharmafect 1 reagent (Dharmacon). ..

    Article Title: Neuronatin regulates pancreatic β cell insulin content and secretion
    Article Snippet: .. For siRNA silencing, INS1E cells ( ) (cultured in RPMI, 10% FBS, 2 mM l -glutamine, 50 μM β-mercaptoethanol, 37°C, 5% CO2 ) were transfected with 50 nM of Silencer Select siRNAs (Nnat , s137247; Sec11a , s80747; both Ambion) using Dharmafect 1 reagent (Dharmacon). ..

    Luciferase:

    Article Title: Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis
    Article Snippet: Per 24-well, the transfection mixture contained 0.5 μg promoter plasmid DNA (StAR, HSD3B2, CYP17A1 and CYP11A1) and 125 ng coregulator plasmid cDNA (e.g. RARB and Nur77) as well as 25 ng Renilla luciferase reporter (pRL-TK) (Promega, Maidson, WI) for endogenous control. .. Transfection medium [DMEM containing L-glutamine and 15 mm HEPES (GIBCO) supplemented with 10% NU-I serum (BD biosciences) and 0.1% selenium/insulin/transferrin (GIBCO)] was changed after 6 h and cells were cultivated in serum free medium for 24 h. Thereafter, cells were grown in the presence or absence of 1 μM ΑΤRA in serum-free medium for another 24 h. Finally, 48 hours after transfection, cells were washed with phosphate buffered saline (PBS) and then assayed for luciferase activity using the Dual Luciferase Reporter Assay system and the protocol by Promega. .. For overexpression studies of RARB, H295R cells were either transfected with the empty vector (pCMV5) or the vector expressing the cDNA of RARB in six wells (1.0 × 106 cells per well; Falcon 3046;BD biosciences) using Lipofectamine 2000 (Invitrogen).

    Activity Assay:

    Article Title: Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis
    Article Snippet: Per 24-well, the transfection mixture contained 0.5 μg promoter plasmid DNA (StAR, HSD3B2, CYP17A1 and CYP11A1) and 125 ng coregulator plasmid cDNA (e.g. RARB and Nur77) as well as 25 ng Renilla luciferase reporter (pRL-TK) (Promega, Maidson, WI) for endogenous control. .. Transfection medium [DMEM containing L-glutamine and 15 mm HEPES (GIBCO) supplemented with 10% NU-I serum (BD biosciences) and 0.1% selenium/insulin/transferrin (GIBCO)] was changed after 6 h and cells were cultivated in serum free medium for 24 h. Thereafter, cells were grown in the presence or absence of 1 μM ΑΤRA in serum-free medium for another 24 h. Finally, 48 hours after transfection, cells were washed with phosphate buffered saline (PBS) and then assayed for luciferase activity using the Dual Luciferase Reporter Assay system and the protocol by Promega. .. For overexpression studies of RARB, H295R cells were either transfected with the empty vector (pCMV5) or the vector expressing the cDNA of RARB in six wells (1.0 × 106 cells per well; Falcon 3046;BD biosciences) using Lipofectamine 2000 (Invitrogen).

    Reporter Assay:

    Article Title: Retinoic acid receptor beta and angiopoietin-like protein 1 are involved in the regulation of human androgen biosynthesis
    Article Snippet: Per 24-well, the transfection mixture contained 0.5 μg promoter plasmid DNA (StAR, HSD3B2, CYP17A1 and CYP11A1) and 125 ng coregulator plasmid cDNA (e.g. RARB and Nur77) as well as 25 ng Renilla luciferase reporter (pRL-TK) (Promega, Maidson, WI) for endogenous control. .. Transfection medium [DMEM containing L-glutamine and 15 mm HEPES (GIBCO) supplemented with 10% NU-I serum (BD biosciences) and 0.1% selenium/insulin/transferrin (GIBCO)] was changed after 6 h and cells were cultivated in serum free medium for 24 h. Thereafter, cells were grown in the presence or absence of 1 μM ΑΤRA in serum-free medium for another 24 h. Finally, 48 hours after transfection, cells were washed with phosphate buffered saline (PBS) and then assayed for luciferase activity using the Dual Luciferase Reporter Assay system and the protocol by Promega. .. For overexpression studies of RARB, H295R cells were either transfected with the empty vector (pCMV5) or the vector expressing the cDNA of RARB in six wells (1.0 × 106 cells per well; Falcon 3046;BD biosciences) using Lipofectamine 2000 (Invitrogen).

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  • 99
    Thermo Fisher m l glutamine
    M L Glutamine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m l glutamine/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m l glutamine - by Bioz Stars, 2021-03
    99/100 stars
      Buy from Supplier

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