m glutamine  (Millipore)

 
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    Name:
    Glutamine
    Description:
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    Catalog Number:
    1294808
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    Structured Review

    Millipore m glutamine
    Glutamine
    This product is provided as delivered and specified by the issuing Pharmacopoeia All information provided in support of this product including SDS and any product information leaflets have been developed and issued under the Authority of the issuing Pharmacopoeia For further information and support please go to the website of the issuing Pharmacopoeia
    https://www.bioz.com/result/m glutamine/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m glutamine - by Bioz Stars, 2021-03
    86/100 stars

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    Incubation:

    Article Title: Treatment of experimental autoimmune uveoretinitis with peroxisome proliferator-activated receptor α agonist fenofibrate
    Article Snippet: The embedded sections were cut on a Vibratome (VT1000S, Leica Microsystems, Heerbrugg, Switzerland) and blocked overnight in normal donkey serum (Jackson Immunoresearch Laboratories, West Grove, PA; 1:20) at 4 °C. .. The sections were then incubated overnight at 4 °C on a rotator with the following primary antibodies: mouse monoclonal antibodies (mAb) to interleukin (IL)-6 (R & D Systems, Minneapolis, MN; 1:100), IL-17 (R & D Systems; 1:100), and glutamine synthetase (GS; Chemicon, Temecula, CA; 1:100), and a rabbit polyclonal Ab to vascular endothelial growth factor (VEGF; Santa Cruz Biotechnology, Santa Cruz, CA; 1:100). .. All antibody solutions were prepared in PBTA (0.1 M PBS (1X; 140 mM NaCl, 2.7 mM KCl, 10 mM PO4 3- , pH 7.4) containing 0.5% bovine serum albumin [BSA; Fisher Scientific, Pittsburgh, PA], 0.1% Triton X-100 [Boehringer-Mannheim, Indianapolis, IN[, and 0.1% sodium azide [Sigma]).

    Article Title: Protective effect of clusterin on rod photoreceptor in rat model of retinitis pigmentosa
    Article Snippet: .. Briefly, sections were incubated with 10% normal donkey serum (NDS) (#017-000-121, Jackson ImmunoResearch Laboratories, West Grove, Pennsylvania, dilution 1:10) for 1 hour at room temperature (RT), then incubated overnight with primary antibodies: goat polyclonal antibody directed against clusterin α (#sc-6420, Santa Cruz Biotechnology, Dallas, TX, dilution 1:1,000); rabbit polyclonal directed against glial fibrillary acidic protein (GFAP)(#G9269, Sigma-Aldrich Corp, St. Louis, MO, dilution 1:1,000); phosphorylated Signaling Transducer and Activator of Transcription 3 (pSTAT3)(#9145S, Cell Signaling Technologies, Danvers, MA, dilution 1:100); mouse monoclonal antibody against Glutamine Synthase (GS) (#MAB302, Millipore, Billerica, Massachusetts, dilution 1:10,000); mouse monoclonal antibody directed against rhodopsin (rho 1D4[ ], dilution 1:1,000). .. Then samples were washed in PBS, and incubated for 2 hours at RT in corresponding secondary antibodies with carboxymethylindocyanine (Cy3)-conjugated affinity-purified donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories, dilution 1:500), Cy3-conjugated affinity-purified donkey anti-mouse IgG (Jackson ImmunoResearch Laboratories, dilution 1:500), Alexa 488-conjugated donkey anti-mouse IgG (Molecular Probes, Eugene, OR, dilution 1:300), or Alexa 488-conjugated donkey anti-goat IgG (Molecular Probes, Eugene, OR, dilution 1:300).

    Blocking Assay:

    Article Title: The disruption of the rod-derived cone viability gene leads to photoreceptor dysfunction and susceptibility to oxidative stress
    Article Snippet: .. Antibodies were diluted in blocking buffer (5 % BSA in PBS-Tween 0.05 %), at a concentration of 1/250 for the rhodopsin antibody (Rho-4D2), 1/100 for FGF2 (Upstate Cell Signaling, Millipore, MA), 1/1000 respectively for recoverin (Millipore, MA), RPE65 (Abcam, Cambridge, UK), GFAP (Dako, Glostrup, Denmark) and Glutamine synthetase (Chemicon, Millipore, MA). .. A concentration of 1/350 was used for HNE antibody (Calbiochem, San Diego, CA) and 1/200 for acrolein antibody (Cosmo bio, Tokyo, Japan).

    Concentration Assay:

    Article Title: The disruption of the rod-derived cone viability gene leads to photoreceptor dysfunction and susceptibility to oxidative stress
    Article Snippet: .. Antibodies were diluted in blocking buffer (5 % BSA in PBS-Tween 0.05 %), at a concentration of 1/250 for the rhodopsin antibody (Rho-4D2), 1/100 for FGF2 (Upstate Cell Signaling, Millipore, MA), 1/1000 respectively for recoverin (Millipore, MA), RPE65 (Abcam, Cambridge, UK), GFAP (Dako, Glostrup, Denmark) and Glutamine synthetase (Chemicon, Millipore, MA). .. A concentration of 1/350 was used for HNE antibody (Calbiochem, San Diego, CA) and 1/200 for acrolein antibody (Cosmo bio, Tokyo, Japan).

    Gas Chromatography:

    Article Title: A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos 1A Combined Metabolomics and Fluxomics Analysis Identifies Steps Limiting Oil Synthesis in Maize Embryos 1 [OPEN]
    Article Snippet: .. Glc, Fru, Gln, Man, polyethylene glycol 4000 (PEG), glyceryl triheptadecanoate (TAG-C17:0), sodium bisulfate, butylamine, methanolic-HCl (3N), deuterium oxide, toluene, dichloromethane, and standards for the metabolomic study were purchased from Sigma-Aldrich. .. [U-13 C4 ]fumarate, [U-13 C2 ]Gly, [1,2-13 C2 ]Glc, [U-13 C6 ]Glc, [U-13 C6 ]Fru, and [U-13 C5 ]Gln were purchased from Isotec.

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  • 97
    Millipore m l glutamine
    M L Glutamine, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m l glutamine/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m l glutamine - by Bioz Stars, 2021-03
    97/100 stars
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