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Thermo Fisher m egta
M Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/Thermo Fisher
Average 99 stars, based on 6 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
99/100 stars

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Transfection:

Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
Article Snippet: .. 3.5 × 105 AD293 cells were seeded in 2ml DMEM (10% FBS) in a 6 well plate, incubated for 24h and transfected with 1μg of plasmid DNA expressing K12-Arg135Thr-eGFP fusion protein using 3.3μl Lipofectamine 2000 (Invitrogen, Paisley, UK) in Optimem serum-free medium following manufacturer’s instructions. .. After 24h the medium was replaced and the cells transfected with siRNA K12-Arg135Thr-5 to a final concentration of 3nM using 6μl of Lipofectamine RNAiMAX (Invitrogen, Paisley, UK) according to the manufacturer’s instructions for 6h.

Isolation:

Article Title: Regulation of inflammation and fibrosis by macrophages in lymphedema
Article Snippet: .. VEGF-C protein expression was quantified in total protein isolated from the tail tissues using ELISA (eBiosciences) as previously described ( ). .. Briefly, skin and subcutaneous tissues were harvested and lysed using the Qiagen DNA/RNA/Protein mini kit using the manufacturer's methods (Qiagen, Valencia, CA).

Enzyme-linked Immunosorbent Assay:

Article Title: Paroxetine differentially modulates LPS-induced TNFα and IL-6 production in mouse macrophages
Article Snippet: .. Supernatants from the treated cells were collected and cytokine analysis for IL-6, and TNFα performed according to manufacturer's instructions using ELISA Kits (eBioscience, San Diego, CA) and as described before [ ]. .. Optical density measurements were taken at 450 nm in an Infinite M1000 PRO plate reader (Tecan, Mannedorf, Switzerland).

Article Title: Regulation of inflammation and fibrosis by macrophages in lymphedema
Article Snippet: .. VEGF-C protein expression was quantified in total protein isolated from the tail tissues using ELISA (eBiosciences) as previously described ( ). .. Briefly, skin and subcutaneous tissues were harvested and lysed using the Qiagen DNA/RNA/Protein mini kit using the manufacturer's methods (Qiagen, Valencia, CA).

Article Title: Differential regulation of TGF-β–induced, ALK-5–mediated VEGF release by SMAD2/3 versus SMAD1/5/8 signaling in glioblastoma
Article Snippet: .. ) were analyzed by ELISA for VEGF levels (eBioscience) and TGF-β1/2 (R & D Systems). .. The pGL3 SBE-4-Luc plasmid (B. Vogelstein) containing 4 copies of the SMAD-binding element (SBE) GTCTAGAC ).

Incubation:

Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
Article Snippet: .. 3.5 × 105 AD293 cells were seeded in 2ml DMEM (10% FBS) in a 6 well plate, incubated for 24h and transfected with 1μg of plasmid DNA expressing K12-Arg135Thr-eGFP fusion protein using 3.3μl Lipofectamine 2000 (Invitrogen, Paisley, UK) in Optimem serum-free medium following manufacturer’s instructions. .. After 24h the medium was replaced and the cells transfected with siRNA K12-Arg135Thr-5 to a final concentration of 3nM using 6μl of Lipofectamine RNAiMAX (Invitrogen, Paisley, UK) according to the manufacturer’s instructions for 6h.

other:

Article Title: Convergence of eicosanoid and integrin biology: 12-lipoxygenase seeks a partner
Article Snippet: Human laminin and EGF were from GIBCO BRL or Sigma Aldrich (St. Louis, MO).

Article Title: Differential requirement for MEK Partner 1 in DU145 prostate cancer cell migration
Article Snippet: Reagents EGF (Invitrogen) and UO126 were from Calbiochem.

Expressing:

Article Title: Regulation of inflammation and fibrosis by macrophages in lymphedema
Article Snippet: .. VEGF-C protein expression was quantified in total protein isolated from the tail tissues using ELISA (eBiosciences) as previously described ( ). .. Briefly, skin and subcutaneous tissues were harvested and lysed using the Qiagen DNA/RNA/Protein mini kit using the manufacturer's methods (Qiagen, Valencia, CA).

Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
Article Snippet: .. 3.5 × 105 AD293 cells were seeded in 2ml DMEM (10% FBS) in a 6 well plate, incubated for 24h and transfected with 1μg of plasmid DNA expressing K12-Arg135Thr-eGFP fusion protein using 3.3μl Lipofectamine 2000 (Invitrogen, Paisley, UK) in Optimem serum-free medium following manufacturer’s instructions. .. After 24h the medium was replaced and the cells transfected with siRNA K12-Arg135Thr-5 to a final concentration of 3nM using 6μl of Lipofectamine RNAiMAX (Invitrogen, Paisley, UK) according to the manufacturer’s instructions for 6h.

Plasmid Preparation:

Article Title: Allele-specific siRNA silencing for the common Keratin 12 founder mutation in Meesmann epithelial corneal dystrophy
Article Snippet: .. 3.5 × 105 AD293 cells were seeded in 2ml DMEM (10% FBS) in a 6 well plate, incubated for 24h and transfected with 1μg of plasmid DNA expressing K12-Arg135Thr-eGFP fusion protein using 3.3μl Lipofectamine 2000 (Invitrogen, Paisley, UK) in Optimem serum-free medium following manufacturer’s instructions. .. After 24h the medium was replaced and the cells transfected with siRNA K12-Arg135Thr-5 to a final concentration of 3nM using 6μl of Lipofectamine RNAiMAX (Invitrogen, Paisley, UK) according to the manufacturer’s instructions for 6h.

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    Thermo Fisher m egta
    M Egta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/Thermo Fisher
    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
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