Structured Review

GE Healthcare m egta
M Egta, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/GE Healthcare
Average 92 stars, based on 5 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
92/100 stars

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Electron Paramagnetic Resonance:

Article Title: Binding of the CYK-4 Subunit of the Centralspindlin Complex Induces a Large Scale Conformational Change in the Kinesin Subunit
Article Snippet: .. ZEN-4m derivatives were exchanged into EPR buffer containing 10 m m HEPES (pH 7.7), 1 m m EGTA (pH 8.0), 1 m m MgCl2 , 250 m m NaCl, and 10% glycerol using a PD-10 desalting column (GE Healthcare) or FPLC Fast Desalting column (GE Healthcare). .. ZEN-4m variants were spin-labeled with (1-oxyl-2,2,5,5-tetramethyl-Δ3-pyrroline-3-methyl) methanethiosulfonate spin label (MTSL, Toronto Research Chemicals) for ∼2 h at 4 °C.

Filtration:

Article Title: Sumoylation of the GTPase Ran by the RanBP2 SUMO E3 Ligase Complex *
Article Snippet: .. Finally, buffer was exchanged to TB (20 m m HEPES, pH 7.3, 110 m m KOAc, 2 m m Mg(OAc)2 , 1 m m EGTA, 1 m m DTT, and 1 μg/ml of each aprotinin, leupeptin, and pepstatin) by gel filtration on an S75 column (GE Healthcare). .. SUMO-Vme purification has been described elsewhere ( ).

Protease Inhibitor:

Article Title: Life Span Extension and Neuronal Cell Protection by Drosophila Nicotinamidase * Nicotinamidase * S⃞
Article Snippet: .. Nicotinamidase/Pyrazinamidase Assay —Cellular and whole fly protein extracts were prepared in extraction buffer containing 50 m m Tris-Cl, pH 7.5, 100 m m NaCl, 1% Triton X-100, 1 m m dithiothreitol, 1 m m EDTA, 1 m m EGTA, 2 m m Na3 VO4 , 50 m m β-glycerophosphate, and a protease inhibitor mixture (GE Healthcare, Piscataway, NJ). ..

Labeling:

Article Title: Functional Analysis of the Osteoarthritis Susceptibility–Associated GDF5 Regulatory Polymorphism
Article Snippet: .. Binding was performed for 15 minutes at room temperature in 16- μ l reaction mixtures containing 60 fmoles (40,000 counts per minute) of labeled probe, 4 μ g of nuclear proteins, 20 m M HEPES (pH 7.9), 2 m M EDTA, 2 m M EGTA, 25% glycerol, and 1 μ g poly(dI-dC) (GE Healthcare). ..

Spectrophotometry:

Article Title: Drosophila Stathmins Bind Tubulin Heterodimers with High and Variable Stoichiometries *
Article Snippet: .. The effect of various D-stathmin variants or fragments on tubulin polymerization in polymerization buffer (50 m m 2-( N -morpholino)ethanesulfonic acid-KOH, pH 6.8, 30% glycerol, 0.5 m m EGTA, 6 m m MgCl2 , and 0.5 m m GTP) was monitored turbidimetrically at 350 nm in an Ultrospec 3000 spectrophotometer (Amersham Biosciences) thermostatted at 37 °C as described before ( ). ..

Binding Assay:

Article Title: Functional Analysis of the Osteoarthritis Susceptibility–Associated GDF5 Regulatory Polymorphism
Article Snippet: .. Binding was performed for 15 minutes at room temperature in 16- μ l reaction mixtures containing 60 fmoles (40,000 counts per minute) of labeled probe, 4 μ g of nuclear proteins, 20 m M HEPES (pH 7.9), 2 m M EDTA, 2 m M EGTA, 25% glycerol, and 1 μ g poly(dI-dC) (GE Healthcare). ..

Article Title: Architecture of the Bacteriophage T4 Activator MotA/Promoter DNA Interaction during Sigma Appropriation *
Article Snippet: .. In the other cases, protein fractions eluting from the phosphocellulose column were dialyzed versus AKTA binding buffer (50 m m potassium phosphate (pH 6.5), 1 m m EDTA, 1 m m EGTA, 1 m m DTT, 1 m m PMSF), loaded onto a HiTrap SP HP cation exchange column (1 ml, GE Healthcare), and eluted with a gradient of 0–500 m m NaCl in AKTA binding buffer (40 ml total). .. When necessary, the MotA proteins were concentrated using Amicon Ultra Centrifugal Filters (3000 molecular weight cutoff) before being buffer-exchanged into MotA GC buffer (200 m m potassium phosphate (pH 6.5), 1 m m DTT, 1 m m benzamidine HCl, 1 m m EDTA, 1 m m EGTA, and 50% glycerol).

Fast Protein Liquid Chromatography:

Article Title: Binding of the CYK-4 Subunit of the Centralspindlin Complex Induces a Large Scale Conformational Change in the Kinesin Subunit
Article Snippet: .. ZEN-4m derivatives were exchanged into EPR buffer containing 10 m m HEPES (pH 7.7), 1 m m EGTA (pH 8.0), 1 m m MgCl2 , 250 m m NaCl, and 10% glycerol using a PD-10 desalting column (GE Healthcare) or FPLC Fast Desalting column (GE Healthcare). .. ZEN-4m variants were spin-labeled with (1-oxyl-2,2,5,5-tetramethyl-Δ3-pyrroline-3-methyl) methanethiosulfonate spin label (MTSL, Toronto Research Chemicals) for ∼2 h at 4 °C.

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    GE Healthcare m egta
    M Egta, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/GE Healthcare
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
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