Structured Review

Fisher Scientific m egta
M Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/Fisher Scientific
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
92/100 stars

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Sonication:

Article Title: A single discrete Rab5-binding site in phosphoinositide 3-kinase β is required for tumor cell invasion
Article Snippet: .. The lipids were dried down under nitrogen gas, lyophilized overnight, resuspended in 250 μl of reaction buffer plus 2 m m EGTA, and sonicated with a cup horn for 10 min (Fisher Scientific, 550 Sonic Dismembrator). .. Lipid vesicles (37 μl) were incubated on ice for 30 min with 1.5 μ m purified Gβ1 γ2 protein or an equal volume of Gβ1 γ2 buffer ( ).

Article Title: Calcium-Independent Activation of Salicylic Acid-Induced Protein Kinase and a 40-Kilodalton Protein Kinase by Hyperosmotic Stress 1
Article Snippet: .. To prepare extracts from treated cells, cells were mixed with two volumes (w/v) of extraction buffer (100 m m 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid [HEPES], pH 7.5, 5 m m EDTA, 5 m m EGTA, 10 m m dithiothreitol [DTT], 10 m m Na3 VO4, 10 m m NaF, 50 m m β-glycerolphosphate, 1 m m phenylmethylsulfonyl fluoride [PMSF], 5 μg/mL antipain, 5 μg/mL aprotinin, 5 μg/mL leupeptin, and 10% [v/v] glycerol) and sonicated (model 550 Sonic Dismembrator, Fisher Scientific, Loughborough, Leicestershire, UK) until all of the cells were disrupted. .. The protein concentration was determined using a protein assay kit (Bio-Rad, Hercules, CA).

Injection:

Article Title: Neurons Lacking Huntingtin Differentially Colonize Brain and Survive in Chimeric Mice
Article Snippet: .. For four additional ES blastocyst injection chimeras the brains were removed and immersion fixed overnight in 0.2% paraformaldehyde in 0.1 m PIPES, pH 6.9, plus 2 m m MgCl2 and 5 m m EGTA, washed in PBS and MgCl2 , cryo-preserved in 30% sucrose plus MgCl2 , embedded in Tissue-Tek OCT compound (Fisher Scientific, Pittsburgh, PA), and frozen. ..

Transferring:

Article Title: Platelet-activating Factor Contributes to Bacillus anthracis Lethal Toxin-associated Damage *
Article Snippet: .. Briefly, the abdominal cavity was washed five times with sterile Hanks balanced salt solution, 1% penicillin-streptomycin, 0.1 m m EGTA (Fisher Scientific) using a sterile Pasteur pipette. .. Following centrifugation, erythrocytes were lysed by resuspension in ice-cold 0.17 m NH4 Cl for 10 min. A 10-fold excess of DMEM solution was then added to make the solution isotonic, the cells were collected by centrifugation, and live cells (trypan blue exclusion and morphological examination) were counted in a hemocytometer chamber.

Incubation:

Article Title: Spatial Learning Impairment, Enhanced CDK5/p35 Activity, and Downregulation of NMDA Receptor Expression in Transgenic Mice Expressing Tau-Tubulin Kinase 1
Article Snippet: .. The immunoprecipitates were washed four times with the lysis buffer, once with kinase buffer (without 32 P-ATP and substrate), and the beads were incubated in 20 μl of kinase buffer containing 20 m m Tris-HCl, pH 7.6, 20 m m MgCl2 , 2 m m MnCl2 , 1 m m EDTA, 1 m m EGTA, 0.1 m m dithiothreitol, 10 μCi of [γ-32 P] ATP and histone-H1 (5 μg, Fisher Scientific) at 30°C for 30 min. ..

Article Title: Coordinated Interactions of Multiple POT1-TPP1 Proteins with Telomere DNA *
Article Snippet: .. Reactions were incubated at room temperature for the time points indicated and then quenched by the addition of 20 m m EGTA (pH 8.0) (Fisher Scientific) combined with heating to 95 °C for 10 min. ..

Lysis:

Article Title: Spatial Learning Impairment, Enhanced CDK5/p35 Activity, and Downregulation of NMDA Receptor Expression in Transgenic Mice Expressing Tau-Tubulin Kinase 1
Article Snippet: .. The immunoprecipitates were washed four times with the lysis buffer, once with kinase buffer (without 32 P-ATP and substrate), and the beads were incubated in 20 μl of kinase buffer containing 20 m m Tris-HCl, pH 7.6, 20 m m MgCl2 , 2 m m MnCl2 , 1 m m EDTA, 1 m m EGTA, 0.1 m m dithiothreitol, 10 μCi of [γ-32 P] ATP and histone-H1 (5 μg, Fisher Scientific) at 30°C for 30 min. ..

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    Fisher Scientific m egta
    M Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/Fisher Scientific
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
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