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FUJIFILM m egta
M Egta, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/FUJIFILM
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-08
92/100 stars

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Flow Cytometry:

Article Title: FGF15/FGFR4 Integrates Growth Factor Signaling with Hepatic Bile Acid Metabolism and Insulin Action
Article Snippet: .. The liver was perfused with Earle's balanced salt solution (EBSS) (Invitrogen) supplemented with 0.5 m m EGTA through the cannulated portal vein at a flow rate of 4 ml/min for 15 min followed by perfusion with EBSS supplemented with 0.3 mg/ml collagenase (Wako Chemicals) and 4.8 m m CaCl2 for 20 min. ..

Gas Chromatography:

Article Title: Biochemical and Immunocytochemical Characterization of Two Types of Myosins in Cultured Tobacco Bright Yellow-2 Cells 1
Article Snippet: .. A solution containing 30 m m KCl, 5 m m EGTA, 6 m m MgCl2 , 1 m m ATP, 0.3 μg/mL RP-labeled F-actin, 0.216 mg/mL Glc oxidase (Wako Pure Chemical, Osaka), 36 μg/mL catalase (Wako), 4.5 mg/mL Glc, 0.6% (w/v) methyl cellulose, 100 m m DTT, and 30 m m PIPES-KOH (pH 7.0) was used for the assay. ..

Autoradiography:

Article Title: Phosphorylation of Cyclin-dependent Kinase 5 (Cdk5) at Tyr-15 Is Inhibited by Cdk5 Activators and Does Not Contribute to the Activation of Cdk5 *
Article Snippet: .. The kinase activity of Cdk5 was measured with histone H1 as a substrate in kinase buffer (10 m m MOPS (pH 6.8), 1 m m MgCl2 , 0.1 m m EDTA, and 0.1 m m EGTA) at 37 °C for 30 min. After SDS-PAGE, phosphorylation was visualized by autoradiography with an imaging plate (FujiFilm, Tokyo, Japan). .. Cortical neurons were transfected with DsRed, p35-myc, and caFyn by a calcium phosphate method at 5 days in vitro (DIV), as described previously , and, 48 h after transfection, neurons were immunostained with anti-myc 4A6, followed by Alexa Fluor 488-conjugated secondary antibodies.

Incubation:

Article Title: Calcium signalling regulates the functions of the bZIP protein VIP1 in touch responses in Arabidopsis thaliana
Article Snippet: .. To examine the effects of calcium signalling inhibitors on subcellular localization of group I bZIP proteins, GFPox, VIP1-GFPox, bZIP59-GFPox and bZIP29-GFPox plants were grown for 10 d on MS agar medium as described, incubated in a hypotonic solution (10 m m NaCl, 10 m m Tris-HCl, pH 8.0) for 0 or 10 min at room temperature, and then incubated in a 10 m m Tris-HCl (pH 8.0) solution containing 10 m m NaCl, 2 m m EDTA, 2 m m EGTA (Wako), 0.5 m m chlorpromazine hydrochloride (Wako), 1 m m GdCl3 (Wako), 1 m m LaCl3 (Wako) and/or 10 m m CaCl2 , for 10 min or 120 min (see legends to , and ) at room temperature. .. GFP signals were then detected using an epifluorescence microscope [BX51 (Olympus) equipped with a DP73 CCD camera (Olympus)].

other:

Article Title: D-loop of Actin Differently Regulates the Motor Function of Myosins II and V *
Article Snippet: Actin-activated ATPase of MMV-HMM was determined by monitoring the absorbance of NADH at 340 nm in the solution containing 56 n m MMV-HMM, 2.35 μ m calmodulin, 20 m m imidazole-HCl (pH 7.4), 4 m m MgCl2 , 40 m m KCl, 1 m m EGTA, 53 μ m phalloidin, 400 μ m NADH (Wako (Osaka, Japan)), 800 μ m phosphoenolpyruvate (Wako), 47 μg/ml pyruvate kinase (Oriental Yeast (Tokyo, Japan)), 12 μg/ml lactate dehydrogenase (Oriental Yeast), 1 m m ATP, 1 m m dithiothreitol, and various concentrations of actin (2.2–39.7 μ m ).

Activity Assay:

Article Title: Phosphorylation of Cyclin-dependent Kinase 5 (Cdk5) at Tyr-15 Is Inhibited by Cdk5 Activators and Does Not Contribute to the Activation of Cdk5 *
Article Snippet: .. The kinase activity of Cdk5 was measured with histone H1 as a substrate in kinase buffer (10 m m MOPS (pH 6.8), 1 m m MgCl2 , 0.1 m m EDTA, and 0.1 m m EGTA) at 37 °C for 30 min. After SDS-PAGE, phosphorylation was visualized by autoradiography with an imaging plate (FujiFilm, Tokyo, Japan). .. Cortical neurons were transfected with DsRed, p35-myc, and caFyn by a calcium phosphate method at 5 days in vitro (DIV), as described previously , and, 48 h after transfection, neurons were immunostained with anti-myc 4A6, followed by Alexa Fluor 488-conjugated secondary antibodies.

Imaging:

Article Title: Phosphorylation of Cyclin-dependent Kinase 5 (Cdk5) at Tyr-15 Is Inhibited by Cdk5 Activators and Does Not Contribute to the Activation of Cdk5 *
Article Snippet: .. The kinase activity of Cdk5 was measured with histone H1 as a substrate in kinase buffer (10 m m MOPS (pH 6.8), 1 m m MgCl2 , 0.1 m m EDTA, and 0.1 m m EGTA) at 37 °C for 30 min. After SDS-PAGE, phosphorylation was visualized by autoradiography with an imaging plate (FujiFilm, Tokyo, Japan). .. Cortical neurons were transfected with DsRed, p35-myc, and caFyn by a calcium phosphate method at 5 days in vitro (DIV), as described previously , and, 48 h after transfection, neurons were immunostained with anti-myc 4A6, followed by Alexa Fluor 488-conjugated secondary antibodies.

Mass Spectrometry:

Article Title: Calcium signalling regulates the functions of the bZIP protein VIP1 in touch responses in Arabidopsis thaliana
Article Snippet: .. To examine the effects of calcium signalling inhibitors on subcellular localization of group I bZIP proteins, GFPox, VIP1-GFPox, bZIP59-GFPox and bZIP29-GFPox plants were grown for 10 d on MS agar medium as described, incubated in a hypotonic solution (10 m m NaCl, 10 m m Tris-HCl, pH 8.0) for 0 or 10 min at room temperature, and then incubated in a 10 m m Tris-HCl (pH 8.0) solution containing 10 m m NaCl, 2 m m EDTA, 2 m m EGTA (Wako), 0.5 m m chlorpromazine hydrochloride (Wako), 1 m m GdCl3 (Wako), 1 m m LaCl3 (Wako) and/or 10 m m CaCl2 , for 10 min or 120 min (see legends to , and ) at room temperature. .. GFP signals were then detected using an epifluorescence microscope [BX51 (Olympus) equipped with a DP73 CCD camera (Olympus)].

SDS Page:

Article Title: Phosphorylation of Cyclin-dependent Kinase 5 (Cdk5) at Tyr-15 Is Inhibited by Cdk5 Activators and Does Not Contribute to the Activation of Cdk5 *
Article Snippet: .. The kinase activity of Cdk5 was measured with histone H1 as a substrate in kinase buffer (10 m m MOPS (pH 6.8), 1 m m MgCl2 , 0.1 m m EDTA, and 0.1 m m EGTA) at 37 °C for 30 min. After SDS-PAGE, phosphorylation was visualized by autoradiography with an imaging plate (FujiFilm, Tokyo, Japan). .. Cortical neurons were transfected with DsRed, p35-myc, and caFyn by a calcium phosphate method at 5 days in vitro (DIV), as described previously , and, 48 h after transfection, neurons were immunostained with anti-myc 4A6, followed by Alexa Fluor 488-conjugated secondary antibodies.

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    FUJIFILM m egta
    M Egta, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/FUJIFILM
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-08
    92/100 stars
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