Structured Review

DuPont de Nemours m egta
M Egta, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/DuPont de Nemours
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
92/100 stars

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Incubation:

Article Title: Norcantharidin-induced apoptosis is via the extracellular signal-regulated kinase and c-Jun-NH2-terminal kinase signaling pathways in human hepatoma HepG2 cells
Article Snippet: .. ERK and JNK kinase activity was determined by incubation of the immunocomplex in 30 μ l of kinase reaction buffer containing 20 m M Tris-HCl, pH 7.5, 5 m M EGTA, 20 m M MgCl2 , 0.5 m M dithiothreitol, 1 μ g MBP (substrate of ERK) or GST-c-Jun (substrate of JNK) peptides, 5 μ Ci ( γ -32 P)ATP (6000 Ci mmol−1 ; DuPont-NEN, Boston, MA, U.S.A.), and 1 μ M ATP for 15 min at 37°C. .. Samples were electrophoresed on 12% SDS–PAGE and then transferred to the PVDF membrane.

Binding Assay:

Article Title: Genetic ablation of ryanodine receptor 2 phosphorylation at Ser-2808 aggravates Ca2+-dependent cardiomyopathy by exacerbating diastolic Ca2+ release
Article Snippet: .. The Ca2+ -dependent [3 H]ryanodine binding was determined with cardiac homogenates plus a standard mixture of 200 m m KCl, 25 m m Tris–50 m m Hepes (pH 7.4), 3 m m MgATP, 1 m m EGTA, 5 n m [3 H]ryanodine (68.4 Ci mmol−1 , Dupont NEN, Wilmington, DE, USA) and CaCl2 . ..

Activity Assay:

Article Title: Norcantharidin-induced apoptosis is via the extracellular signal-regulated kinase and c-Jun-NH2-terminal kinase signaling pathways in human hepatoma HepG2 cells
Article Snippet: .. ERK and JNK kinase activity was determined by incubation of the immunocomplex in 30 μ l of kinase reaction buffer containing 20 m M Tris-HCl, pH 7.5, 5 m M EGTA, 20 m M MgCl2 , 0.5 m M dithiothreitol, 1 μ g MBP (substrate of ERK) or GST-c-Jun (substrate of JNK) peptides, 5 μ Ci ( γ -32 P)ATP (6000 Ci mmol−1 ; DuPont-NEN, Boston, MA, U.S.A.), and 1 μ M ATP for 15 min at 37°C. .. Samples were electrophoresed on 12% SDS–PAGE and then transferred to the PVDF membrane.

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    DuPont de Nemours m egta
    M Egta, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/DuPont de Nemours
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
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