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Bio-Rad m egta
M Egta, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/Bio-Rad
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
92/100 stars

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Centrifugation:

Article Title: Immediate and delayed VEGF-mediated NO synthesis in endothelial cells: Role of PI3K, PKC and PLC pathways
Article Snippet: .. Cells were lysed (20 m M Tris-HCl pH 7.4, 150 m M NaCl, 1.2% Triton X-100, 1 m M EGTA, 1 m M , phenylmethylsulphonyl fluoride, 0.15 u ml−1 aprotinin, 10 μg ml−1 leupeptin, 1 m M NaVO3 , 1 m M Microcystin LR), the plates were scraped, the lysate clarified by centrifugation, and the protein concentration of the supernatant determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). .. Proteins (100–150 μg) were separated on a 7.5% SDS–PAGE and transblotted onto an immunobilon-P polyvinylidene difluoride membranes (Milipore, Bedford, MA, USA).

Protease Inhibitor:

Article Title: Arsenic Promotes Ubiquitinylation and Lysosomal Degradation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Chloride Channels in Human Airway Epithelial Cells *
Article Snippet: .. The cell pellet was lysed in immunoprecipitation buffer containing 50 m m HEPES, pH 7.4, 150 m m NaCl, 10% glycerol, 5 m m MgCl2 , 5 m m EDTA, 1 m m EGTA, 1% Triton X-100 (Bio-Rad), Complete protease inhibitor tablet without EDTA (Roche Applied Science), and PhosSTOP tablet (Roche Applied Science) for 30 min at 4 °C and then centrifuged at 14,000 × g for 10 min at 4 °C. .. The supernatant was collected in a new tube and rotated at 4 °C for 2 h. After centrifuging at 14,000 × g for 15 min, the lysates were added to protein A-Sepharose bead antibody complexes. c-Cbl was immunoprecipitated with rabbit c-Cbl C-15 antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Titration:

Article Title: Calmodulin Mediates the Ca2+-Dependent Regulation of Cx44 Gap Junctions
Article Snippet: .. For Ca2+ stoichiometric titration, the protein or peptide stock solutions (∼1 mM) were pretreated with 50 μ M EGTA to remove residual Ca2+ ions and then extensively dialyzed against buffers pretreated with Chelex-100 resin (BioRad, Hercules, CA). .. Protein or peptides were then diluted to the desired concentration (5–8 μ M) with Chelex-treated buffers consisting of 50 mM Tris, 100 mM KCl at pH 7.5 for Ca2+ titration ( λ ex = 277 nm, λ em = 307 nm).

Immunoprecipitation:

Article Title: Arsenic Promotes Ubiquitinylation and Lysosomal Degradation of Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Chloride Channels in Human Airway Epithelial Cells *
Article Snippet: .. The cell pellet was lysed in immunoprecipitation buffer containing 50 m m HEPES, pH 7.4, 150 m m NaCl, 10% glycerol, 5 m m MgCl2 , 5 m m EDTA, 1 m m EGTA, 1% Triton X-100 (Bio-Rad), Complete protease inhibitor tablet without EDTA (Roche Applied Science), and PhosSTOP tablet (Roche Applied Science) for 30 min at 4 °C and then centrifuged at 14,000 × g for 10 min at 4 °C. .. The supernatant was collected in a new tube and rotated at 4 °C for 2 h. After centrifuging at 14,000 × g for 15 min, the lysates were added to protein A-Sepharose bead antibody complexes. c-Cbl was immunoprecipitated with rabbit c-Cbl C-15 antibody (Santa Cruz Biotechnology, Santa Cruz, CA).

Protein Concentration:

Article Title: Immediate and delayed VEGF-mediated NO synthesis in endothelial cells: Role of PI3K, PKC and PLC pathways
Article Snippet: .. Cells were lysed (20 m M Tris-HCl pH 7.4, 150 m M NaCl, 1.2% Triton X-100, 1 m M EGTA, 1 m M , phenylmethylsulphonyl fluoride, 0.15 u ml−1 aprotinin, 10 μg ml−1 leupeptin, 1 m M NaVO3 , 1 m M Microcystin LR), the plates were scraped, the lysate clarified by centrifugation, and the protein concentration of the supernatant determined by using a protein assay kit (Bio-Rad, Hercules, CA, USA). .. Proteins (100–150 μg) were separated on a 7.5% SDS–PAGE and transblotted onto an immunobilon-P polyvinylidene difluoride membranes (Milipore, Bedford, MA, USA).

Silver Staining:

Article Title: The 19S proteasome is directly involved in the regulation of heterochromatin spreading in fission yeast
Article Snippet: .. The proteins were eluted with elution buffer (2 m m ATP, 10 m m Tris-Cl (pH 8.0), 1 m m MgAc, 1 m m imidazole, 3 m m EGTA, 150 m m NaCl, 0.1% Nonidet P-40, 10 m m β-mercaptoethanol, and 10% glycerol), resolved by 4–15% Mini-PROTEAN® TGXTM precast gel (Bio-Rad), and visualized with silver staining. .. The wild-type ORFs encoding Rpt3, Rpt4, and Rpt6 were subjected to random mutagenesis by error-prone PCR using GeneMorph II random mutagenesis kits (Stratagene) according to the manufacturer's protocol.

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    Bio-Rad m egta
    M Egta, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/Bio-Rad
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
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