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Beckman Coulter m egta
M Egta, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m egta/product/Beckman Coulter
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m egta - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Kinesin-2 KIF3AB Exhibits Novel ATPase Characteristics *
Article Snippet: .. KIF3AB at 6, 12, and 25 μ m (heterodimer concentration) or protein standards at 25 μ m were loaded onto the HPLC gel filtration column (SuperoseTM 10/300, GE Healthcare Life Sciences) with elution at 20 m m HEPES, pH 7.2, with KOH, 0.1 m m EDTA, 0.1 m m EGTA, 5 m m magnesium acetate, 50 m m potassium acetate, 1 m m DTT plus 100 m m NaCl using a Beckman Coulter System Gold HPLC. .. Protein elution was determined by intrinsic fluorescence detection (Jasco FP-2020, Victoria, British Columbia) as described previously ( , ). was used to determine the partition coefficients of standards relative to KIF3AB, where Ve is the elution volume, Vt is the total volume, and V 0 is the void volume.

Centrifugation:

Article Title: Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1 [OPEN]
Article Snippet: .. The supernatant was centrifuged at 100,000 g for 2 h. The pellets were suspended in a microsome suspension buffer (5% [w/v] Suc, 20 m m Tris-acetate, pH 7.5, 0.5 m m EGTA, 1× protease inhibitor and 4 m m EDTA) and subjected to centrifugation in 10% to 50% Suc linear gradient, 20 m m Tris-HCl (pH 7.5), 0.5 m m EGTA, 1× protease inhibitor, 4 m m EDTA at 100,000 g overnight in a SW41Ti rotor (Beckman) for fractionation. ..

Article Title: Myr 8, A Novel Unconventional Myosin Expressed during Brain Development Associates with the Protein Phosphatase Catalytic Subunits 1α and 1γ1
Article Snippet: .. Postnatal day 8 brain was homogenized (10% w/v) by 10 passes in a Brendler glass–Teflon pestle homogenizer (1200 rpm) in medium containing 20 m m 3-( N -morpholino) propane sulfonic acid (MOPS), pH 7.4, 75 m m KCl, 2.5 m m MgCl2 , 1 m m EGTA, 1 m m dithiothreitol, 0.4 m m phenylmethylsulfonyl fluoride, 0.5 m m ATP, and 5 μg/ml each of antipain, leupeptin, pepstatin A, and aprotinin, and a supernatant fraction obtained by centrifugation (100,000 × g for 15 min, 4°C; Beckman Type 60 ti rotor). .. Supernatant samples (100 μg protein) were supplemented with 50 μg human platelet non-muscle actin (Cytoskeleton, Denver, CO) and 50 m m glucose in the presence or absence of 0.5 U hexokinase (St. Louis, MO).

Luciferase:

Article Title: Glibenclamide depletes ATP in renal proximal tubular cells by interfering with mitochondrial metabolism
Article Snippet: .. The supernatant was diluted 25 times and to 50 μ l of this solution 500 μ l assay medium was added, containing 50 m M glycylglycine, 7.5 m M dithiothreitol, 2 m M EGTA, 2 m M MgCl2 , 0.04% (w v−1 ) bovine serum albumin (BSA), 10 μ g ml−1 luciferin, and 2000 U ml−1 luciferase at pH 8.0, and measured by using a liquid scintillation counter (Beckman LS 6000 LL, U.S.A.). .. ATP concentrations were calculated by comparing scintillation counts with a calibration curve of various concentrations of ATP in perchloric acid : neutralization buffer 1 : 1.25.

Filtration:

Article Title: Kinesin-2 KIF3AB Exhibits Novel ATPase Characteristics *
Article Snippet: .. KIF3AB at 6, 12, and 25 μ m (heterodimer concentration) or protein standards at 25 μ m were loaded onto the HPLC gel filtration column (SuperoseTM 10/300, GE Healthcare Life Sciences) with elution at 20 m m HEPES, pH 7.2, with KOH, 0.1 m m EDTA, 0.1 m m EGTA, 5 m m magnesium acetate, 50 m m potassium acetate, 1 m m DTT plus 100 m m NaCl using a Beckman Coulter System Gold HPLC. .. Protein elution was determined by intrinsic fluorescence detection (Jasco FP-2020, Victoria, British Columbia) as described previously ( , ). was used to determine the partition coefficients of standards relative to KIF3AB, where Ve is the elution volume, Vt is the total volume, and V 0 is the void volume.

Protease Inhibitor:

Article Title: Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1 [OPEN]
Article Snippet: .. The supernatant was centrifuged at 100,000 g for 2 h. The pellets were suspended in a microsome suspension buffer (5% [w/v] Suc, 20 m m Tris-acetate, pH 7.5, 0.5 m m EGTA, 1× protease inhibitor and 4 m m EDTA) and subjected to centrifugation in 10% to 50% Suc linear gradient, 20 m m Tris-HCl (pH 7.5), 0.5 m m EGTA, 1× protease inhibitor, 4 m m EDTA at 100,000 g overnight in a SW41Ti rotor (Beckman) for fractionation. ..

Construct:

Article Title: Calmodulin Bound to the First IQ Motif Is Responsible for Calcium-dependent Regulation of Myosin 5a *
Article Snippet: .. Purified myosin 5a constructs were incubated with rabbit skeletal actin (10 μ m ) in a 50 μl solution consisting of 20 m m MOPS-KOH, pH7.0, 50 m m NaCl, 1 m m MgCl2 , 1 m m DTT, 12 μ m CaM WT, or mutants, and 1 m m EGTA (1 m m EGTA was replaced with 0.87 m m EGTA and 1 m m CaCl2 for pCa4 conditions) at 0 °C for 10 min and then centrifuged in a benchtop ultracentrifuge (Beckman Optima MAX-XP) at 80,000 rpm for 10 min at 0 °C. .. The pellets were resuspended in 50 μl of SDS-gel sample buffer (containing 5 m m EGTA) and subjected to SDS-PAGE.

Purification:

Article Title: Calmodulin Bound to the First IQ Motif Is Responsible for Calcium-dependent Regulation of Myosin 5a *
Article Snippet: .. Purified myosin 5a constructs were incubated with rabbit skeletal actin (10 μ m ) in a 50 μl solution consisting of 20 m m MOPS-KOH, pH7.0, 50 m m NaCl, 1 m m MgCl2 , 1 m m DTT, 12 μ m CaM WT, or mutants, and 1 m m EGTA (1 m m EGTA was replaced with 0.87 m m EGTA and 1 m m CaCl2 for pCa4 conditions) at 0 °C for 10 min and then centrifuged in a benchtop ultracentrifuge (Beckman Optima MAX-XP) at 80,000 rpm for 10 min at 0 °C. .. The pellets were resuspended in 50 μl of SDS-gel sample buffer (containing 5 m m EGTA) and subjected to SDS-PAGE.

Concentration Assay:

Article Title: Kinesin-2 KIF3AB Exhibits Novel ATPase Characteristics *
Article Snippet: .. KIF3AB at 6, 12, and 25 μ m (heterodimer concentration) or protein standards at 25 μ m were loaded onto the HPLC gel filtration column (SuperoseTM 10/300, GE Healthcare Life Sciences) with elution at 20 m m HEPES, pH 7.2, with KOH, 0.1 m m EDTA, 0.1 m m EGTA, 5 m m magnesium acetate, 50 m m potassium acetate, 1 m m DTT plus 100 m m NaCl using a Beckman Coulter System Gold HPLC. .. Protein elution was determined by intrinsic fluorescence detection (Jasco FP-2020, Victoria, British Columbia) as described previously ( , ). was used to determine the partition coefficients of standards relative to KIF3AB, where Ve is the elution volume, Vt is the total volume, and V 0 is the void volume.

Incubation:

Article Title: Calmodulin Bound to the First IQ Motif Is Responsible for Calcium-dependent Regulation of Myosin 5a *
Article Snippet: .. Purified myosin 5a constructs were incubated with rabbit skeletal actin (10 μ m ) in a 50 μl solution consisting of 20 m m MOPS-KOH, pH7.0, 50 m m NaCl, 1 m m MgCl2 , 1 m m DTT, 12 μ m CaM WT, or mutants, and 1 m m EGTA (1 m m EGTA was replaced with 0.87 m m EGTA and 1 m m CaCl2 for pCa4 conditions) at 0 °C for 10 min and then centrifuged in a benchtop ultracentrifuge (Beckman Optima MAX-XP) at 80,000 rpm for 10 min at 0 °C. .. The pellets were resuspended in 50 μl of SDS-gel sample buffer (containing 5 m m EGTA) and subjected to SDS-PAGE.

Fractionation:

Article Title: Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1Two Trichome Birefringence-Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice 1 [OPEN]
Article Snippet: .. The supernatant was centrifuged at 100,000 g for 2 h. The pellets were suspended in a microsome suspension buffer (5% [w/v] Suc, 20 m m Tris-acetate, pH 7.5, 0.5 m m EGTA, 1× protease inhibitor and 4 m m EDTA) and subjected to centrifugation in 10% to 50% Suc linear gradient, 20 m m Tris-HCl (pH 7.5), 0.5 m m EGTA, 1× protease inhibitor, 4 m m EDTA at 100,000 g overnight in a SW41Ti rotor (Beckman) for fractionation. ..

Chick Chorioallantoic Membrane Assay:

Article Title: Calmodulin Bound to the First IQ Motif Is Responsible for Calcium-dependent Regulation of Myosin 5a *
Article Snippet: .. Purified myosin 5a constructs were incubated with rabbit skeletal actin (10 μ m ) in a 50 μl solution consisting of 20 m m MOPS-KOH, pH7.0, 50 m m NaCl, 1 m m MgCl2 , 1 m m DTT, 12 μ m CaM WT, or mutants, and 1 m m EGTA (1 m m EGTA was replaced with 0.87 m m EGTA and 1 m m CaCl2 for pCa4 conditions) at 0 °C for 10 min and then centrifuged in a benchtop ultracentrifuge (Beckman Optima MAX-XP) at 80,000 rpm for 10 min at 0 °C. .. The pellets were resuspended in 50 μl of SDS-gel sample buffer (containing 5 m m EGTA) and subjected to SDS-PAGE.

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  • 92
    Beckman Coulter m egta
    M Egta, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta/product/Beckman Coulter
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    86
    Beckman Coulter m k egta buffer
    M K Egta Buffer, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m k egta buffer/product/Beckman Coulter
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m k egta buffer - by Bioz Stars, 2020-09
    86/100 stars
      Buy from Supplier

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