m egta am  (Thermo Fisher)


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    Structured Review

    Thermo Fisher m egta am
    M Egta Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta am/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta am - by Bioz Stars, 2020-09
    85/100 stars

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    Article Title: Exocytosis at the Ribbon Synapse of Retinal Bipolar Cells Studied in Patches of Presynaptic Membrane
    Article Snippet: .. In some experiments, terminals were loaded with the Ca2+ chelator EGTA by incubation in 0.1 or 0.2 m m EGTA-AM (0.1% DMSO; Molecular Probes, Eugene, OR) for 15–30 min. Changes in the capacitance of the membrane patch (Δ C m ) were made using the piecewise linear technique ( ) with a dual-phase digital lock-in amplifier (model SR850 DSP; Stanford Research Systems, Stanford, CA) controlled through the RS232 interface by macros written in Igor Pro software. .. A sinusoidal command generated by the lock-in amplifier was added to the command potential generated by the ITC-16 interface using a summing amplifier, both inputs of which could be gated by a transistor–transistor logic (TTL) signal.

    Article Title: Directional Bleb Formation in Spherical Cells under Temperature Gradient
    Article Snippet: .. To chelate intracellular Ca2+ , cells were incubated in HBS containing 30 μ M EGTA-AM (Thermo Fisher Scientific) or 50 μ M BAPTA-AM (Dojindo Laboratories, Kumamoto, Japan) and 1 μ M fluo-4-AM for 30 min at room temperature, and then observed in Ca2+ -free HBS [140 mM NaCl, 5 mM KCl, 1 mM MgCl2 , 1 mM Na2 HPO4 , 10 mM HEPES, 5 mM D(+)-glucose, 500 μM EGTA, pH 7.4]. .. Fluo-4-AM (1 mM), EGTA-AM (30 mM), and BAPTA-AM (50 mM) stocks in DMSO were stored at −20°C.

    Article Title: Two Actions of Calcium Regulate the Supply of Releasable Vesicles at the Ribbon Synapse of Retinal Bipolar Cells
    Article Snippet: .. In some experiments terminals were loaded with the Ca2+ chelator EGTA by incubation in 0.1 or 0.2 m m EGTA-AM (0.1% DMSO; Molecular Probes, Eugene, OR) for 15–20 min. All measurements are given as mean ± SEM. .. The approach we used to measure the size of the RRP is derived from the behavior shown in Figure .

    Article Title: Two Actions of Calcium Regulate the Supply of Releasable Vesicles at the Ribbon Synapse of Retinal Bipolar Cells
    Article Snippet: .. In some experiments terminals were loaded with the Ca2+ chelator EGTA by incubation in 0.1 or 0.2 m m EGTA-AM (0.1% DMSO; Molecular Probes, Eugene, OR) for 15–20 min. All measurements are given as mean ± SEM. .. The solution in the patch pipette contained (in m m ): 110 caesium gluconate, 4 MgCl2 , 3 Na2 ATP, 1 Na2 GTP, 10 TEACl, 0.4 BAPTA, and 20 HEPES, with 250 μg/ml nystatin (260 mOsm/l, pH 7.2).

    Software:

    Article Title: Exocytosis at the Ribbon Synapse of Retinal Bipolar Cells Studied in Patches of Presynaptic Membrane
    Article Snippet: .. In some experiments, terminals were loaded with the Ca2+ chelator EGTA by incubation in 0.1 or 0.2 m m EGTA-AM (0.1% DMSO; Molecular Probes, Eugene, OR) for 15–30 min. Changes in the capacitance of the membrane patch (Δ C m ) were made using the piecewise linear technique ( ) with a dual-phase digital lock-in amplifier (model SR850 DSP; Stanford Research Systems, Stanford, CA) controlled through the RS232 interface by macros written in Igor Pro software. .. A sinusoidal command generated by the lock-in amplifier was added to the command potential generated by the ITC-16 interface using a summing amplifier, both inputs of which could be gated by a transistor–transistor logic (TTL) signal.

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  • 85
    Thermo Fisher m egta am
    M Egta Am, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m egta am/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m egta am - by Bioz Stars, 2020-09
    85/100 stars
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