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Promega m dtt
M Dtt, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Promega
Average 99 stars, based on 12 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
99/100 stars

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Related Articles

High Performance Liquid Chromatography:

Article Title: Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications
Article Snippet: .. Trypsin (mass spectrometry grade; Promega, cat. no. V511A; store at 4 °C) DL-DTT (Promega, cat. no. V3155) (store at −20 °C) Acrylamide (Fluka, cat. no. 01696) (store at 4 °C, in dark) Octyl β-D-glucopyranoside (Sigma-Aldrich, cat. no. 03757) (store at −20 °C) Ammonium bicarbonate (Thermo Fisher, cat. no. A643-500) Trizma base (Sigma-Aldrich, cat. no. T6066) Water, HPLC grade (Thermo Fisher, cat. no. W5-4) Acetonitrile (Thermo Fisher, cat. no. A988-4) ! .. Use appropriate protective attire (lab coats, safety glasses and latex gloves) and pipette under fume hood.

Sample Prep:

Article Title: H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
Article Snippet: .. Sample preparation Frozen cell pellets were thawed, washed with phosphate-buffered saline, resuspended in 500 µL of lysis buffer (pH 7.6) containing 2% (w/v) SDS (L4390; Sigma-Aldrich), 0.1 M DTT (V3155; Promega), 0.1 M Trizma® base (T1503; Sigma-Aldrich), and vortexed. .. The cell suspension was transferred to a 2 mL screw cap microtube (522-Q; Thermo Fisher Scientific) containing 0.1 mm glass beads (11079101; BioSpec Products).

Knock-Out:

Article Title: Generating kidney organoids from human pluripotent stem cells
Article Snippet: .. 2-Mercaptoethanol (55 mM) (Thermo Fisher Scientific, cat. no. 21985-023) Antibiotic-Antimycotic (Thermo Fisher Scientific, cat. no. 15240-062) bFGF (Merck, cat. no. GF003-AF) BSA (Sigma Aldrich, cat. no. A3311-10G) CHIR99021 (R & D, cat. no. 4423/10) DMEM high glucose (Thermo Fisher Scientific, cat. no. 11995-073) DMEM/F-12 (Thermo Fisher Scientific, cat. no. 11320-082) DMSO (Sigma Aldrich, cat. no. D5879) DTT, 100 mM (Promega, cat. no. P1171) Dulbecco's phosphate-buffered saline (DPBS) (Thermo Fisher Scientific, cat. no. 14190-144) FGF9 (R & D, cat. no. 273-F9-025) Foetal Bovine Serum (FBS) (Interpath Services, cat. no. SFBSF) Gelatin (Sigma Aldrich, cat. no. G9391) GlutaMAX Supplement (Thermo Fisher Scientific, cat. no. 35050-061) Glycerol (Sigma Aldrich, cat. no. G9012-500ML) Heparin (Sigma Aldrich, cat. no. H4784-250MG) hESC-qualified Matrigel, LDEV-Free (Corning, cat. no. 354277) Knockout Serum Replacement (Thermo Fisher Scientific, cat. no. 10828-028) Non-Essential Amino Acids (Thermo Fisher Scientific, cat. no. 11140-050) Penicillin/Streptomycin (Thermo Fisher Scientific, cat. no. 15070-063) Recombinant human serum albumin, 10% (wt/vol) (Novozymes, 230-005) STEMdiff APEL Medium (Stem Cell Technologies, cat. no. 5210) TrypLE Select (Thermo Fisher Scientific, cat. no. 12563-029) Trypsin EDTA, 0.05% (Thermo Fisher Scientific, cat. no. 25300-054) Paraformaldehyde (Sigma-Aldrich, cat. no. 158127) Triton X-100 (TritonX) (Sigma-Aldrich, cat. no. T9284-500ML) Donkey serum (Merck Millipore, cat. no. S30-100ML) DAPI (6-Diamidino-2-Phenylindole Dihydrochloride) (Thermo Fisher Scientific, cat. no. D1306) Appropriate antibodies to detect proteins of interest (see for primary antibodies we have successfully used). .. 175 cm2 tissue culture flask (Nunc, cat. no. 159910) 25 cm2 tissue culture flask (Nunc, cat. no. 156367) 6-well transwell cell culture plate, 0.4 μm pore polyester membrane (Corning, cat no. 3450) Benchtop centrifuge (Thermo Scientific, Heraeus Pico 17) Biological safety cabinet (LAF Technologies, TOP-SAFE 1.2) CO2 incubators (Thermo Scientific, Heracell 150i) Conical tubes, 15 ml (Corning, cat. no. 352096) Conical tubes, 50 ml (Corning, cat. no. 352070) Freezing container (Nalgene, Mr. Frosty) Glass-Bottom Dish, 35mm (Mattek, cat. no. P35G-0-14-C) Inverted contrasting tissue culture microscope (Nikon, TS100) Laser confocal microscope (Zeiss, LSM780) Media storage bottle, 500 ml (Corning, cat. no. 430282) Pipetboy (Integra Biosciences, PIPETBOY pro) Pipettes (Gilson, P10, P200 and P1000 PIPETMAN Classic) Serological pipettes, 10 ml (Corning, cat. no. 357551) Stericup 0.22 μm filter unit (Merck Millipore, cat. no. SCGPU05RE) Sterile filter pipette tips (1000, 200, wide bore 200 and 10 μl; Molecular BioProducts, cat. nos.

Modification:

Article Title: Profiling post-translational modifications of histones in human monocyte-derived macrophages
Article Snippet: .. Dithiothreitol (DTT, molecular grade) and modified trypsin (sequencing grade) were purchased from Promega Corporation (Madison, WI, USA). .. Sample preparation Primary human monocytes were obtained by leukophoresis from donors who were seronegative for HIV-1, HIV-2, and hepatitis B virus.

other:

Article Title: The Casein Kinase II catalytic subunit CSNK2A1 interacts with TTBK2 to regulate ciliary trafficking and stability
Article Snippet: Samples were supplemented with 40μL 10% SDS in 50 mM TEAB, then reduced with 10 mM dithiolthreitol for 30min at 80C and alkylated with 20 mM iodoacetamide for 30min at room temperature.

Mass Spectrometry:

Article Title: Integrated mass spectrometry–based analysis of plasma glycoproteins and their glycan modifications
Article Snippet: .. Trypsin (mass spectrometry grade; Promega, cat. no. V511A; store at 4 °C) DL-DTT (Promega, cat. no. V3155) (store at −20 °C) Acrylamide (Fluka, cat. no. 01696) (store at 4 °C, in dark) Octyl β-D-glucopyranoside (Sigma-Aldrich, cat. no. 03757) (store at −20 °C) Ammonium bicarbonate (Thermo Fisher, cat. no. A643-500) Trizma base (Sigma-Aldrich, cat. no. T6066) Water, HPLC grade (Thermo Fisher, cat. no. W5-4) Acetonitrile (Thermo Fisher, cat. no. A988-4) ! .. Use appropriate protective attire (lab coats, safety glasses and latex gloves) and pipette under fume hood.

Lysis:

Article Title: The changing mouse embryo transcriptome at whole tissue and single-cell resolution
Article Snippet: .. Lysis buffer: 8.6 μ L water, 1 μ L C1 loading buffer, 2.4 μ L Smartseq2 oligo dT primer (10mM), 2.4 μ L Clontech 10mM dNTPs, 2 μ L ERCC spikes (AM4456740) (diluted 1:40,000 in UltraPure H2 O (InVitrogen 10977023) containing carrier tRNA (AM7119) at 200 pg/μ L, RNAse inhibitor (Clontech 2313A) at 1 units/μ L and DTT (Promega P1171) at 1 mM), 0.5 μ L 100mM DTT, 2.6 μ L Clontech single-cell reaction buffer. .. Reverse transcription reaction: 5.6 μ L Clontech 10x transcription buffer, 0.6 μ L C1 loading buffer, 5.6 μ L Smart-seq2 TSO (10mM), 0.4. μ L Clontech RNAse inhibitor, 2.8 μ L Clontech SMARTScribe.

Article Title: H2 drives metabolic rearrangements in gas-fermenting Clostridium autoethanogenum
Article Snippet: .. Sample preparation Frozen cell pellets were thawed, washed with phosphate-buffered saline, resuspended in 500 µL of lysis buffer (pH 7.6) containing 2% (w/v) SDS (L4390; Sigma-Aldrich), 0.1 M DTT (V3155; Promega), 0.1 M Trizma® base (T1503; Sigma-Aldrich), and vortexed. .. The cell suspension was transferred to a 2 mL screw cap microtube (522-Q; Thermo Fisher Scientific) containing 0.1 mm glass beads (11079101; BioSpec Products).

Sequencing:

Article Title: Profiling post-translational modifications of histones in human monocyte-derived macrophages
Article Snippet: .. Dithiothreitol (DTT, molecular grade) and modified trypsin (sequencing grade) were purchased from Promega Corporation (Madison, WI, USA). .. Sample preparation Primary human monocytes were obtained by leukophoresis from donors who were seronegative for HIV-1, HIV-2, and hepatitis B virus.

Staining:

Article Title: Single molecule linear analysis of DNA in nano-channel labeled with sequence specific fluorescent probes
Article Snippet: .. DNA loading into nano-channel All the DNA (4 ng/µl) samples were stained with intercalating dye YOYO-1 iodide (Invitrogen, Carlsbad, CA; Cat #Y3601) (1 molecule/10 bp) in presence of 0.4 M DTT (Promega Inc, Madison, WI, USA; Cat #V3151). .. The sample was diluted by two times using the flow buffer consisting of 1× TBE, 3.6% Tween and 10% polyvinyl pyrrolidone (PVP).

Recombinant:

Article Title: Generating kidney organoids from human pluripotent stem cells
Article Snippet: .. 2-Mercaptoethanol (55 mM) (Thermo Fisher Scientific, cat. no. 21985-023) Antibiotic-Antimycotic (Thermo Fisher Scientific, cat. no. 15240-062) bFGF (Merck, cat. no. GF003-AF) BSA (Sigma Aldrich, cat. no. A3311-10G) CHIR99021 (R & D, cat. no. 4423/10) DMEM high glucose (Thermo Fisher Scientific, cat. no. 11995-073) DMEM/F-12 (Thermo Fisher Scientific, cat. no. 11320-082) DMSO (Sigma Aldrich, cat. no. D5879) DTT, 100 mM (Promega, cat. no. P1171) Dulbecco's phosphate-buffered saline (DPBS) (Thermo Fisher Scientific, cat. no. 14190-144) FGF9 (R & D, cat. no. 273-F9-025) Foetal Bovine Serum (FBS) (Interpath Services, cat. no. SFBSF) Gelatin (Sigma Aldrich, cat. no. G9391) GlutaMAX Supplement (Thermo Fisher Scientific, cat. no. 35050-061) Glycerol (Sigma Aldrich, cat. no. G9012-500ML) Heparin (Sigma Aldrich, cat. no. H4784-250MG) hESC-qualified Matrigel, LDEV-Free (Corning, cat. no. 354277) Knockout Serum Replacement (Thermo Fisher Scientific, cat. no. 10828-028) Non-Essential Amino Acids (Thermo Fisher Scientific, cat. no. 11140-050) Penicillin/Streptomycin (Thermo Fisher Scientific, cat. no. 15070-063) Recombinant human serum albumin, 10% (wt/vol) (Novozymes, 230-005) STEMdiff APEL Medium (Stem Cell Technologies, cat. no. 5210) TrypLE Select (Thermo Fisher Scientific, cat. no. 12563-029) Trypsin EDTA, 0.05% (Thermo Fisher Scientific, cat. no. 25300-054) Paraformaldehyde (Sigma-Aldrich, cat. no. 158127) Triton X-100 (TritonX) (Sigma-Aldrich, cat. no. T9284-500ML) Donkey serum (Merck Millipore, cat. no. S30-100ML) DAPI (6-Diamidino-2-Phenylindole Dihydrochloride) (Thermo Fisher Scientific, cat. no. D1306) Appropriate antibodies to detect proteins of interest (see for primary antibodies we have successfully used). .. 175 cm2 tissue culture flask (Nunc, cat. no. 159910) 25 cm2 tissue culture flask (Nunc, cat. no. 156367) 6-well transwell cell culture plate, 0.4 μm pore polyester membrane (Corning, cat no. 3450) Benchtop centrifuge (Thermo Scientific, Heraeus Pico 17) Biological safety cabinet (LAF Technologies, TOP-SAFE 1.2) CO2 incubators (Thermo Scientific, Heracell 150i) Conical tubes, 15 ml (Corning, cat. no. 352096) Conical tubes, 50 ml (Corning, cat. no. 352070) Freezing container (Nalgene, Mr. Frosty) Glass-Bottom Dish, 35mm (Mattek, cat. no. P35G-0-14-C) Inverted contrasting tissue culture microscope (Nikon, TS100) Laser confocal microscope (Zeiss, LSM780) Media storage bottle, 500 ml (Corning, cat. no. 430282) Pipetboy (Integra Biosciences, PIPETBOY pro) Pipettes (Gilson, P10, P200 and P1000 PIPETMAN Classic) Serological pipettes, 10 ml (Corning, cat. no. 357551) Stericup 0.22 μm filter unit (Merck Millipore, cat. no. SCGPU05RE) Sterile filter pipette tips (1000, 200, wide bore 200 and 10 μl; Molecular BioProducts, cat. nos.

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    Promega m dtt
    M Dtt, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Promega
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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