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Nacalai m dtt
M Dtt, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Nacalai
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
92/100 stars

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Centrifugation:

Article Title: Crystallization and preliminary X-ray structural studies of human prouroguanylin
Article Snippet: .. The recombinant 2K mutant, which was obtained as insoluble material, was dissolved in 30 ml 0.1 M Tris–HCl pH 8.0 containing 8 M urea and 10 m M DTT and kept at 323 K for 1 h. After centrifugation, the supernatant was applied onto a column of Cosmosil 140C18 -OPN (10 ml; Nacarai Tesque, Kyoto, Japan) which was pre-equilibrated and washed with 50 ml 20% acetonitrile in 0.05% trifluoroacetic acid (TFA). ..

Radio Immunoprecipitation:

Article Title: Anks4b, a Novel Target of HNF4? Protein, Interacts with GRP78 Protein and Regulates Endoplasmic Reticulum Stress-induced Apoptosis in Pancreatic ?-Cells *
Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 0.1% SDS, 1% Nonidet P-40, 5 m m EDTA, 0.5% sodium deoxycholate, 20 μg/ml Na3 VO4 , 10 m m NaF, 1 m m PMSF, 2 m m DTT, and protease inhibitor mixture (1/100)) from Nacalai Tesque. .. Total protein was separated by SDS-polyacrylamide gel electrophoresis, transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA), and probed with primary antibodies.

Mutagenesis:

Article Title: Crystallization and preliminary X-ray structural studies of human prouroguanylin
Article Snippet: .. The recombinant 2K mutant, which was obtained as insoluble material, was dissolved in 30 ml 0.1 M Tris–HCl pH 8.0 containing 8 M urea and 10 m M DTT and kept at 323 K for 1 h. After centrifugation, the supernatant was applied onto a column of Cosmosil 140C18 -OPN (10 ml; Nacarai Tesque, Kyoto, Japan) which was pre-equilibrated and washed with 50 ml 20% acetonitrile in 0.05% trifluoroacetic acid (TFA). ..

Protease Inhibitor:

Article Title: Anks4b, a Novel Target of HNF4? Protein, Interacts with GRP78 Protein and Regulates Endoplasmic Reticulum Stress-induced Apoptosis in Pancreatic ?-Cells *
Article Snippet: .. Cells were lysed in radioimmunoprecipitation assay buffer (50 m m Tris-HCl (pH 8.0), 150 m m NaCl, 0.1% SDS, 1% Nonidet P-40, 5 m m EDTA, 0.5% sodium deoxycholate, 20 μg/ml Na3 VO4 , 10 m m NaF, 1 m m PMSF, 2 m m DTT, and protease inhibitor mixture (1/100)) from Nacalai Tesque. .. Total protein was separated by SDS-polyacrylamide gel electrophoresis, transferred to a polyvinylidene fluoride (PVDF) membrane (Immobilon-P; Millipore, Bedford, MA), and probed with primary antibodies.

Article Title: The Plasma Membrane H+-ATPase AHA1 Plays a Major Role in Stomatal Opening in Response to Blue Light 1
Article Snippet: .. Guard cell protoplasts were treated with 26S proteasome inhibitors MG132 (50 µ m ; Sigma-Aldrich) and Ser protease inhibitor apronitin (10 µ m ; Calbiochem) with 2 m m DTT (Nacalai Tesque), 1 m m Nα -tosyl-lys chloromethyl ketone (TLCK; Calbiochem), and 1% protease inhibitor cocktail Set III (Calbiochem) by incubation of the protoplasts with each chemical for 30 min at 24°C. .. Guard cell protoplasts were also treated with 5 m m PMSF (Nacalai Tesque) for 30 min at 24°C in the same manner.

Article Title: Crystal Structure of Heterodimeric Hexaprenyl Diphosphate Synthase from Micrococcus luteus B-P 26 Reveals That the Small Subunit Is Directly Involved in the Product Chain Length Regulation *
Article Snippet: .. The harvested cell pellet was suspended in suspending buffer (50 m m sodium phosphate (pH 7.0), 300 m m NaCl, 1 m m DTT) containing 10 μl/ml protease/inhibitor cocktail (NACALAI TESQUE). .. The suspension was sonicated on ice, and the cell debris was removed by centrifugation for 60 min. at 18,000 rpm (30,000 × g ).

Incubation:

Article Title: The Plasma Membrane H+-ATPase AHA1 Plays a Major Role in Stomatal Opening in Response to Blue Light 1
Article Snippet: .. Guard cell protoplasts were treated with 26S proteasome inhibitors MG132 (50 µ m ; Sigma-Aldrich) and Ser protease inhibitor apronitin (10 µ m ; Calbiochem) with 2 m m DTT (Nacalai Tesque), 1 m m Nα -tosyl-lys chloromethyl ketone (TLCK; Calbiochem), and 1% protease inhibitor cocktail Set III (Calbiochem) by incubation of the protoplasts with each chemical for 30 min at 24°C. .. Guard cell protoplasts were also treated with 5 m m PMSF (Nacalai Tesque) for 30 min at 24°C in the same manner.

Article Title: Phosphatidylcholine Affects Inner Membrane Protein Translocases of Mitochondria *
Article Snippet: .. In brief, the cell wall was disrupted by incubation with 0.1 m Tris/HCl, pH 9.4, 10 m m DTT and subsequent treatment with zymolyase (Nacalai Tesque) in 1.2 m sorbitol, 20 m m KPi , pH 7.2. .. The generated spheroplasts were resuspended in homogenizing buffer (0.6 m sorbitol, 10 m m Tris/HCl, pH 7.4, 1 m m EDTA, 1 m m PMSF, and 0.2% (w/v) BSA) and disintegrated by using a Dounce homogenizer.

Lysis:

Article Title: The Soluble Form of LOTUS inhibits Nogo Receptor-Mediated Signaling by Interfering with the Interaction Between Nogo Receptor Type 1 and p75 Neurotrophin Receptor
Article Snippet: .. The treated cells were washed with cold TBS once and treated with Rho lysis buffer containing 50 m m Tris, pH 7.5, 150 m m NaCl, 30 m m MgCl2 , 1% Nonidet P-40, 1 m m sodium orthovanadate (Sigma-Aldrich), 10 m m NaF, 1 m m DTT, 10 μg/ml leupeptin (Nacalai Tesque), and 0.1 U/ml aprotinin (Sigma-Aldrich). .. After centrifugation at 16,900 × g for 10 min, cell lysate was incubated with GST-fused Rhotekin-Rho binding domain beads (Cytoskeleton, catalog #RT02) for 45 min at 4°C with mild rotation.

Recombinant:

Article Title: Crystallization and preliminary X-ray structural studies of human prouroguanylin
Article Snippet: .. The recombinant 2K mutant, which was obtained as insoluble material, was dissolved in 30 ml 0.1 M Tris–HCl pH 8.0 containing 8 M urea and 10 m M DTT and kept at 323 K for 1 h. After centrifugation, the supernatant was applied onto a column of Cosmosil 140C18 -OPN (10 ml; Nacarai Tesque, Kyoto, Japan) which was pre-equilibrated and washed with 50 ml 20% acetonitrile in 0.05% trifluoroacetic acid (TFA). ..

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    Nacalai m dithiothreitol
    M Dithiothreitol, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Nacalai
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Nacalai m dtt
    M Dtt, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Nacalai
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    91
    Nacalai m dithiothreitol dtt
    M Dithiothreitol Dtt, supplied by Nacalai, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol dtt/product/Nacalai
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol dtt - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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