Structured Review

Fisher Scientific m dtt
M Dtt, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Fisher Scientific
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Lysis:

Article Title: Characterization of the SRP68/72 interface of human signal recognition particle by systematic site-directed mutagenesis
Article Snippet: .. Frozen cells were resuspended in 6.5 mL of ice-cold 50 m M Na-phosphate, pH 8.0, 200 m M NaCl, 5 m M EDTA, 5 m M DTT (lysis buffer) and sonicated, using a Sonic Dismembrator Model 300 (Fisher Scientific) at a setting of 35 percent, three times for 15 s with 15 s intervals on ice. .. The lysate was subjected at 4°C to a low-speed centrifugation at 15,000 rpm using a Sorvall SS34 rotor for 20 min.

Chromatography:

Article Title: Novel hyperoxidation resistance motifs in 2-Cys peroxiredoxins
Article Snippet: .. All proteins were prereduced with 10 m m DTT (Fisher Scientific) for 30 min, and Bio-Spin disposable chromatography columns containing Bio-Gel P-6 Gel resin (Bio-Rad) were used for desalting. ..

Sonication:

Article Title: Characterization of the SRP68/72 interface of human signal recognition particle by systematic site-directed mutagenesis
Article Snippet: .. Frozen cells were resuspended in 6.5 mL of ice-cold 50 m M Na-phosphate, pH 8.0, 200 m M NaCl, 5 m M EDTA, 5 m M DTT (lysis buffer) and sonicated, using a Sonic Dismembrator Model 300 (Fisher Scientific) at a setting of 35 percent, three times for 15 s with 15 s intervals on ice. .. The lysate was subjected at 4°C to a low-speed centrifugation at 15,000 rpm using a Sorvall SS34 rotor for 20 min.

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