Structured Review

Carl Roth GmbH m dtt
M Dtt, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Carl Roth GmbH
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
92/100 stars

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Protease Inhibitor:

Article Title: A Membrane-bound Hemoglobin from Gills of the Green Shore Crab Carcinus maenas *
Article Snippet: .. Cells were harvested by centrifugation at 4000 × g for 30 min and resuspended in 50 m m Tris/HCl, pH 8.0, 1 m m DTT, 1 m m MgCl2 , 8 μg/ml DNase, 4 μg/ml RNase supplemented with Pefabloc SC protease inhibitor (Roth) and Complete protease inhibitor mixture (Roche Applied Science). .. Bacteria were disrupted by sonication (5 × 1 min), and the sample was incubated for 2 h at 37 °C to digest the DNA and RNA, followed by a centrifugation for 1 h at 10,000 × g and 4 °C to remove the cell debris.

Article Title: A Membrane-bound Hemoglobin from Gills of the Green Shore Crab Carcinus maenas *
Article Snippet: .. Selected tissues from C. maenas were homogenized in 10 m m Tris/HCl, pH 7.4, 10 m m NaCl, 5 m m MgCl2 , 1 m m DTT, 0.1% Nonidet P40, 1 m m Pefabloc SC protease inhibitor (Roth) and Complete protease inhibitor mixture (Roche Applied Science). ..

Article Title: Oxygen Supply from the Bird's Eye Perspective
Article Snippet: .. Tissues were homogenized in 10 m m Tris/HCl, pH 7.4, 10 m m NaCl, 5 m m MgCl2 , 1 m m DTT, 1 m m Pefabloc SC protease inhibitor (Carl Roth, Karlsruhe, Germany) and CompleteTM protease inhibitor mix (Roche Applied Science). ..

Centrifugation:

Article Title: A Membrane-bound Hemoglobin from Gills of the Green Shore Crab Carcinus maenas *
Article Snippet: .. Cells were harvested by centrifugation at 4000 × g for 30 min and resuspended in 50 m m Tris/HCl, pH 8.0, 1 m m DTT, 1 m m MgCl2 , 8 μg/ml DNase, 4 μg/ml RNase supplemented with Pefabloc SC protease inhibitor (Roth) and Complete protease inhibitor mixture (Roche Applied Science). .. Bacteria were disrupted by sonication (5 × 1 min), and the sample was incubated for 2 h at 37 °C to digest the DNA and RNA, followed by a centrifugation for 1 h at 10,000 × g and 4 °C to remove the cell debris.

Lysis:

Article Title: Neuronal Dysfunction in iPSC-Derived Medium Spiny Neurons from Chorea-Acanthocytosis Patients Is Reversed by Src Kinase Inhibition and F-Actin Stabilization
Article Snippet: .. The supernatant was collected and the same volume of RIPA lysis buffer was applied containing 50 m m Tris/HCl, pH 7.4, 1% Triton X-100, 1% sodium deoxycholate (Sigma-Aldrich), 0.1% SDS (Roth), 0.15 m NaCl (Roth), 1 m m EDTA (Roth), 1 m m DTT (Roth), and 1 m m sodium orthovanadate (Sigma-Aldrich). .. Cells were scrapped off and incubated in the lysis buffer for 15 min on ice.

Polyacrylamide Gel Electrophoresis:

Article Title: Different pathotypes of the sunflower downy mildew pathogen Plasmopara halstedii all contain isometric virions †
Article Snippet: .. The purified virions were dissociated by heating the samples twice to 95 °C for 5 min in 25 m m Tris buffer, pH 7.5, with 10 m m DTT and 2.5 µL PAGE loading buffer (Roti‐Load, Carl Roth, Karlsruhe, Germany) per 25 µL sample volume. .. Protein electrophoresis was performed on 13% polyacrylamide gels (60 V, 2.5 h, aqueous running buffer: 50 m m Tris, 384 m m glycine and 0.1% SDS, pH 8.3; Coomassie blue staining).

Purification:

Article Title: Different pathotypes of the sunflower downy mildew pathogen Plasmopara halstedii all contain isometric virions †
Article Snippet: .. The purified virions were dissociated by heating the samples twice to 95 °C for 5 min in 25 m m Tris buffer, pH 7.5, with 10 m m DTT and 2.5 µL PAGE loading buffer (Roti‐Load, Carl Roth, Karlsruhe, Germany) per 25 µL sample volume. .. Protein electrophoresis was performed on 13% polyacrylamide gels (60 V, 2.5 h, aqueous running buffer: 50 m m Tris, 384 m m glycine and 0.1% SDS, pH 8.3; Coomassie blue staining).

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    Carl Roth GmbH m dtt
    M Dtt, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Carl Roth GmbH
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
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