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Boehringer Mannheim m dtt
M Dtt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Boehringer Mannheim
Average 92 stars, based on 10 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
92/100 stars

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Lysis:

Article Title: The human papillomavirus E7 oncoprotein can uncouple cellular differentiation and proliferation in human keratinocytes by abrogating p21Cip1-mediated inhibition of cdk2
Article Snippet: .. The immunocomplexes were washed three times in 0.1% NP-40 lysis buffer and twice in 2× kinase buffer (50 m m Tris HCl at pH 7.4, 20 m m MgCl2 ) and then resuspended in 2× kinase buffer supplemented with 1 m m DTT and 100 μ m ATP (Boehringer Mannheim). ..

Article Title: Increased Binding Activity at an Antioxidant-Responsive Element in the Metallothionein-1 Promoter and Rapid Induction of Metallothionein-1 and -2 in Response to Cerebral Ischemia and Reperfusion
Article Snippet: .. The cortices were homogenized using a glass homogenizer (Kontes, Vineland, NJ) in 3 ml of cell lysis buffer consisting of 10 m m HEPES, 1.5 m m MgCl2 , 10 m m KCl, 0.5 m m DTT, pH 7.9, and the following protease/phosphatase inhibitors: 0.2 m m dimethylsulfonyl fluoride, 10 μg/ml aprotinin, 10 μg/ml leupeptin, 1.5 μg/ml pepastatin, 1 μg/ml phosphoramidon, and 1 m m Na3 VO4 (all from Boehringer Mannheim, Mannheim, Germany). .. After 15 min on ice, 180 μl of a solution of 10% Nonidet P-40 (Fluka, Natick, MA) was added, and the homogenate was vortexed for 10 sec and centrifuged at 1500 × g for 5 min at 4°C.

Article Title: The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context
Article Snippet: .. Abp1p SH3 domain affinity resin was incubated with 0.4, 0.8, or 1.6 mg of whole-cell extract and lysis buffer [100 m m Tris–HCl (pH 7.9), 250 m m NaCl, 5 m m EDTA, 50 m m NaF, 0.1% NP-40, 1 m m DTT, 10% glycerol, 1 EDTA-free protease inhibitor cocktail tablet (Boehringer Mannheim, Indianapolis) per 10 ml] to a total volume of 100 μl. ..

Incubation:

Article Title: Pentoxifylline decreases in vivo and in vitro tumour necrosis factor-alpha (TNF-?) production in lepromatous leprosy patients with erythema nodosum leprosum (ENL)
Article Snippet: .. After cooling on ice, a solution containing 5× RT buffer, 40 U RNAsin (Promega Biotec, Madison, WI), 10 m m DTT (Boehringer Mannheim Biochemicals, Indianapolis, IN), 100 μg/ml acetylated bovine serum albumin (BSA; Promega Biotec), 250 μ m of each dNTP (Perkin Elmer Cetus, Emeryville, CA), and SuperScript II RNAse H− reverse transcriptase (G ibco ) at 200 U, in a final volume of 20 μl, was added to the tubes, which were incubated for 1 h at 42°C. ..

Article Title: c-fos Controls the “Private Pathway” of Light-Induced Apoptosis of Retinal Photoreceptors
Article Snippet: .. For EMSA, 2–5 μg (5 μl) of protein of nuclear extract were incubated on ice for 20 min with 19 μl of 5 m m MgCl2 , 0.1 m m EDTA, 0.75 m m DTT, 7.5% glycerol, and 0.05% NP-40 containing 24 μg of BSA and 2 μg of poly(dI·dC) (Boehringer Mannheim). .. Radiolabeled oligonucleotide (1 μl) was added, and incubation was continued for another 20 min. Protein–DNA complexes were resolved at 150 V on a 0.75 or 1.5 mm 6% polyacrylamide gel using 0.25× Tris Borat EDTA buffer and visualized on x-ray film.

Article Title: The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context
Article Snippet: .. Abp1p SH3 domain affinity resin was incubated with 0.4, 0.8, or 1.6 mg of whole-cell extract and lysis buffer [100 m m Tris–HCl (pH 7.9), 250 m m NaCl, 5 m m EDTA, 50 m m NaF, 0.1% NP-40, 1 m m DTT, 10% glycerol, 1 EDTA-free protease inhibitor cocktail tablet (Boehringer Mannheim, Indianapolis) per 10 ml] to a total volume of 100 μl. ..

Protease Inhibitor:

Article Title: The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context
Article Snippet: .. Abp1p SH3 domain affinity resin was incubated with 0.4, 0.8, or 1.6 mg of whole-cell extract and lysis buffer [100 m m Tris–HCl (pH 7.9), 250 m m NaCl, 5 m m EDTA, 50 m m NaF, 0.1% NP-40, 1 m m DTT, 10% glycerol, 1 EDTA-free protease inhibitor cocktail tablet (Boehringer Mannheim, Indianapolis) per 10 ml] to a total volume of 100 μl. ..

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    Boehringer Mannheim m dtt
    M Dtt, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Boehringer Mannheim
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim m dithiothreitol
    M Dithiothreitol, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dithiothreitol/product/Boehringer Mannheim
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dithiothreitol - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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