n m proteasome  (BioVision)

 
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    Name:
    Z DEVD FMK
    Description:
    A synthetic peptide that irreversibly inhibits activity of DEVD dependent caspases e g caspase 3 and caspase 7 and blocks apoptosis The inhibitor is designed as a methyl ester to facilitate cell permeability The product can be used for both in vitro and in vivo studies CAUTION If the intended use is on purified or recombinant enzymes esterase should be added to generate free carboxyl groups
    Catalog Number:
    1143-1
    Price:
    None
    Size:
    1 mg
    Category:
    Caspase Inhibitor
    Quantity:
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    Structured Review

    BioVision n m proteasome
    Z DEVD FMK
    A synthetic peptide that irreversibly inhibits activity of DEVD dependent caspases e g caspase 3 and caspase 7 and blocks apoptosis The inhibitor is designed as a methyl ester to facilitate cell permeability The product can be used for both in vitro and in vivo studies CAUTION If the intended use is on purified or recombinant enzymes esterase should be added to generate free carboxyl groups
    https://www.bioz.com/result/n m proteasome/product/BioVision
    Average 90 stars, based on 166 article reviews
    Price from $9.99 to $1999.99
    n m proteasome - by Bioz Stars, 2020-09
    90/100 stars

    Images

    1) Product Images from "Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *"

    Article Title: Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.720151

    Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (
    Figure Legend Snippet: Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (

    Techniques Used: Inhibition, Activity Assay

    2) Product Images from "Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *"

    Article Title: Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.720151

    Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (
    Figure Legend Snippet: Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (

    Techniques Used: Inhibition, Activity Assay

    3) Product Images from "Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells"

    Article Title: Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1512869112

    Caspase inhibition limits satellite cell differentiation and perturbs muscle regeneration. ( A ) IF of single fibers isolated from Myf5-Cre/Rosa-YFP mice cultured in 20 µM z.DEVD.fmk ( Right ) or DMSO (control) ( Left ) stained with Pax7 (red), GFP
    Figure Legend Snippet: Caspase inhibition limits satellite cell differentiation and perturbs muscle regeneration. ( A ) IF of single fibers isolated from Myf5-Cre/Rosa-YFP mice cultured in 20 µM z.DEVD.fmk ( Right ) or DMSO (control) ( Left ) stained with Pax7 (red), GFP

    Techniques Used: Inhibition, Cell Differentiation, Isolation, Mouse Assay, Cell Culture, Staining

    Pax7 protein is cleaved by caspase 3 at a cryptic cleavage site. ( A ) Differentiation time course of primary myoblasts treated with the caspase 3 peptide inhibitor z.DEVD.fmk (20 µM) or DMSO control. Lysates were probed for αPax7 (upper
    Figure Legend Snippet: Pax7 protein is cleaved by caspase 3 at a cryptic cleavage site. ( A ) Differentiation time course of primary myoblasts treated with the caspase 3 peptide inhibitor z.DEVD.fmk (20 µM) or DMSO control. Lysates were probed for αPax7 (upper

    Techniques Used:

    4) Product Images from "Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *"

    Article Title: Substrate Ubiquitination Controls the Unfolding Ability of the Proteasome *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M116.720151

    Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (
    Figure Legend Snippet: Ubp6 inhibition does not cause lower unfolding ability of Rsp5-ubiquitinated substrates. a, deubiquitinase activity (hydrolysis of 0.5 μ m ) of ubiquitin-AMC of 40 n m proteasome ( black squares ) in the absence or presence of 100 μ m IU1 (

    Techniques Used: Inhibition, Activity Assay

    5) Product Images from "Exposure of Phosphatidylserine by Xk-related Protein Family Members during Apoptosis *"

    Article Title: Exposure of Phosphatidylserine by Xk-related Protein Family Members during Apoptosis *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.583419

    Caspase-mediated Xkr cleavage. A , representations of wild-type (Xkr4 and Xkr9) and caspase-resistant mutant forms (Xkr4 1DA and Xkr9 2DA) fused to GFP. TM , transmembrane. B and C , caspase cleavage of Xkr4 and Xkr9. The solubilized membrane fraction of
    Figure Legend Snippet: Caspase-mediated Xkr cleavage. A , representations of wild-type (Xkr4 and Xkr9) and caspase-resistant mutant forms (Xkr4 1DA and Xkr9 2DA) fused to GFP. TM , transmembrane. B and C , caspase cleavage of Xkr4 and Xkr9. The solubilized membrane fraction of

    Techniques Used: Mutagenesis

    6) Product Images from "MFN2 Couples Glutamate Excitotoxicity and Mitochondrial Dysfunction in Motor Neurons *MFN2 Couples Glutamate Excitotoxicity and Mitochondrial Dysfunction in Motor Neurons * ♦"

    Article Title: MFN2 Couples Glutamate Excitotoxicity and Mitochondrial Dysfunction in Motor Neurons *MFN2 Couples Glutamate Excitotoxicity and Mitochondrial Dysfunction in Motor Neurons * ♦

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.617167

    Degradation of MFN2 by calpain in response to glutamate challenge in primary spinal cord motor neurons. A, motor neurons (DIV7) were treated with 25 μ m glutamate for 24 h, and mRNA levels of mitochondrial dynamic proteins were assayed by real
    Figure Legend Snippet: Degradation of MFN2 by calpain in response to glutamate challenge in primary spinal cord motor neurons. A, motor neurons (DIV7) were treated with 25 μ m glutamate for 24 h, and mRNA levels of mitochondrial dynamic proteins were assayed by real

    Techniques Used:

    Degradation of MFN2 by calpain in vitro . A, representative immunoblot of recombinant human MFN2 in the presence of different units of μ-calpain for 30 min at 30 °C. Cleavage of recombinant of MFN2 could be totally inhibited by calpain
    Figure Legend Snippet: Degradation of MFN2 by calpain in vitro . A, representative immunoblot of recombinant human MFN2 in the presence of different units of μ-calpain for 30 min at 30 °C. Cleavage of recombinant of MFN2 could be totally inhibited by calpain

    Techniques Used: In Vitro, Recombinant

    7) Product Images from "Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner"

    Article Title: Schmallenberg virus induces apoptosis in Vero cell line via extrinsic and intrinsic pathways in a time and dose dependent manner

    Journal: The Journal of Veterinary Medical Science

    doi: 10.1292/jvms.18-0582

    Effects of SBV infection on activation of caspase-3, -8 and -9 in Vero cells. Activation of caspases are detected by using colorimetric kits. Samples were collected at 2, 6, 12, 18, 24, 36, 48 hr post-infection. Results are calculated as the mean ± SEM versus mock infected group. Asterisk indicates statistically significance ( P
    Figure Legend Snippet: Effects of SBV infection on activation of caspase-3, -8 and -9 in Vero cells. Activation of caspases are detected by using colorimetric kits. Samples were collected at 2, 6, 12, 18, 24, 36, 48 hr post-infection. Results are calculated as the mean ± SEM versus mock infected group. Asterisk indicates statistically significance ( P

    Techniques Used: Infection, Activation Assay

    8) Product Images from "Self-assembled glycopeptide nanofibers as modulators of galectin-1 bioactivity"

    Article Title: Self-assembled glycopeptide nanofibers as modulators of galectin-1 bioactivity

    Journal: Cellular and molecular bioengineering

    doi: 10.1007/s12195-015-0399-2

    LacNAc-Q11 nanofibers inhibited galectin-1-mediated Jurkat T cell agglutination and apoptosis a) Cytotoxicity of GlcNAc-Q11 and LacNAc-Q11 nanofibers. b) Bright-field photomicrographs of Jurkat T cell agglutination in culture media with or without Gal-1 in the presence of 1 mM Q11 nanofibers with 0, 100, or 250 μM LacNAc, or 250 μM β-lactose. c) Bright-field and fluorescent photomicrographs of Jurkat T cells stained with Annexin V-eGFP to assess phosphatidylserine exposure, an early marker of apoptosis, in media supplemented with 10 μM Gal-1 alone or with 1 mM Q11 nanofibers having 100 μM LacNAc-Q11. d) Metabolic activity of Jurkat T cells in media supplemented with 10 μM Gal-1 plus 1 mM Q11 nanofibers with 0-250 μM LacNAc or 500 μM thiodigalactoside, a known galectin inhibitor and stable analog of β-lactose. * represents p
    Figure Legend Snippet: LacNAc-Q11 nanofibers inhibited galectin-1-mediated Jurkat T cell agglutination and apoptosis a) Cytotoxicity of GlcNAc-Q11 and LacNAc-Q11 nanofibers. b) Bright-field photomicrographs of Jurkat T cell agglutination in culture media with or without Gal-1 in the presence of 1 mM Q11 nanofibers with 0, 100, or 250 μM LacNAc, or 250 μM β-lactose. c) Bright-field and fluorescent photomicrographs of Jurkat T cells stained with Annexin V-eGFP to assess phosphatidylserine exposure, an early marker of apoptosis, in media supplemented with 10 μM Gal-1 alone or with 1 mM Q11 nanofibers having 100 μM LacNAc-Q11. d) Metabolic activity of Jurkat T cells in media supplemented with 10 μM Gal-1 plus 1 mM Q11 nanofibers with 0-250 μM LacNAc or 500 μM thiodigalactoside, a known galectin inhibitor and stable analog of β-lactose. * represents p

    Techniques Used: Agglutination, Staining, Marker, Activity Assay

    9) Product Images from "Exposure of Phosphatidylserine by Xk-related Protein Family Members during Apoptosis *"

    Article Title: Exposure of Phosphatidylserine by Xk-related Protein Family Members during Apoptosis *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M114.583419

    Caspase-mediated Xkr cleavage. A , representations of wild-type (Xkr4 and Xkr9) and caspase-resistant mutant forms (Xkr4 1DA and Xkr9 2DA) fused to GFP. TM , transmembrane. B and C , caspase cleavage of Xkr4 and Xkr9. The solubilized membrane fraction of
    Figure Legend Snippet: Caspase-mediated Xkr cleavage. A , representations of wild-type (Xkr4 and Xkr9) and caspase-resistant mutant forms (Xkr4 1DA and Xkr9 2DA) fused to GFP. TM , transmembrane. B and C , caspase cleavage of Xkr4 and Xkr9. The solubilized membrane fraction of

    Techniques Used: Mutagenesis

    10) Product Images from "Estrogen receptor α mediates the effects of notoginsenoside R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes"

    Article Title: Estrogen receptor α mediates the effects of notoginsenoside R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2015.3394

    TNFα stimulates the activation of caspase-3 and apoptosis in H9c2 cardiomyocytes. (A) H9c2 cells were cultured with the indicated concentrations (0–16 ng/ml) of TNF-α for 24 h. The activity of caspase-3 was measured using a fluorometric assay and expressed as the fold-change compared with the control (n=6 per group; # P
    Figure Legend Snippet: TNFα stimulates the activation of caspase-3 and apoptosis in H9c2 cardiomyocytes. (A) H9c2 cells were cultured with the indicated concentrations (0–16 ng/ml) of TNF-α for 24 h. The activity of caspase-3 was measured using a fluorometric assay and expressed as the fold-change compared with the control (n=6 per group; # P

    Techniques Used: Activation Assay, Cell Culture, Activity Assay

    Effects of LPS, NG-R1 and/or ER antagonists on the apoptosis of H9c2 cardiomyocytes. (A) ERα mediates the effects of NG-R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes. Cells were pre-incubated with ICI or MPP for 30 min prior to treatment with or without NG-R1 (25 μ M) for 1 h, followed by LPS (20 μ g/ml) for 24 h. The cells were subsequently fixed and subjected to TUNEL and DAPI staining (magnification, ×200). (B) TUNEL apoptotic index was determined by calculating the ratio of TUNEL-positive cells to total cells. (C) Caspase-3 activity was measured using a fluorometric assay, and expressed as the fold-change compared with the control. The data are presented as the mean ± standard error of the mean (n=8 per group; # P
    Figure Legend Snippet: Effects of LPS, NG-R1 and/or ER antagonists on the apoptosis of H9c2 cardiomyocytes. (A) ERα mediates the effects of NG-R1 on endotoxin-induced inflammatory and apoptotic responses in H9c2 cardiomyocytes. Cells were pre-incubated with ICI or MPP for 30 min prior to treatment with or without NG-R1 (25 μ M) for 1 h, followed by LPS (20 μ g/ml) for 24 h. The cells were subsequently fixed and subjected to TUNEL and DAPI staining (magnification, ×200). (B) TUNEL apoptotic index was determined by calculating the ratio of TUNEL-positive cells to total cells. (C) Caspase-3 activity was measured using a fluorometric assay, and expressed as the fold-change compared with the control. The data are presented as the mean ± standard error of the mean (n=8 per group; # P

    Techniques Used: Incubation, TUNEL Assay, Staining, Activity Assay

    11) Product Images from "Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells"

    Article Title: Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1512869112

    Caspase inhibition limits satellite cell differentiation and perturbs muscle regeneration. ( A ) IF of single fibers isolated from Myf5-Cre/Rosa-YFP mice cultured in 20 µM z.DEVD.fmk ( Right ) or DMSO (control) ( Left ) stained with Pax7 (red), GFP
    Figure Legend Snippet: Caspase inhibition limits satellite cell differentiation and perturbs muscle regeneration. ( A ) IF of single fibers isolated from Myf5-Cre/Rosa-YFP mice cultured in 20 µM z.DEVD.fmk ( Right ) or DMSO (control) ( Left ) stained with Pax7 (red), GFP

    Techniques Used: Inhibition, Cell Differentiation, Isolation, Mouse Assay, Cell Culture, Staining

    Pax7 protein is cleaved by caspase 3 at a cryptic cleavage site. ( A ) Differentiation time course of primary myoblasts treated with the caspase 3 peptide inhibitor z.DEVD.fmk (20 µM) or DMSO control. Lysates were probed for αPax7 (upper
    Figure Legend Snippet: Pax7 protein is cleaved by caspase 3 at a cryptic cleavage site. ( A ) Differentiation time course of primary myoblasts treated with the caspase 3 peptide inhibitor z.DEVD.fmk (20 µM) or DMSO control. Lysates were probed for αPax7 (upper

    Techniques Used:

    12) Product Images from "Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells"

    Article Title: Aldehyde dehydrogenase 1A1 confers intrinsic and acquired resistance to gemcitabine in human pancreatic adenocarcinoma MIA PaCa-2 cells

    Journal: International Journal of Oncology

    doi: 10.3892/ijo.2012.1516

    Aldehyde dehydrogenase 1A1 (ALDH1A1)-small interfering RNA (siRNA) increases apoptotic cell death by gemcitabine (GEM) in MIA PaCa-2/GR cells. MIA PaCa-2 cells were transfected with ALDH1A1-siRNA or control-siRNA for 48 h, and then further treated with 1 μ M GEM for 48 h. (A) Western blot analysis for the detection of poly(ADP-ribose) polymerase (PARP) cleavage was used to determine apoptotic cell death. (B) A caspase-3 activity assay for the detection of caspase-3 activity was also used to determine apoptotic cell death. Error bars represent the standard deviation. ** P
    Figure Legend Snippet: Aldehyde dehydrogenase 1A1 (ALDH1A1)-small interfering RNA (siRNA) increases apoptotic cell death by gemcitabine (GEM) in MIA PaCa-2/GR cells. MIA PaCa-2 cells were transfected with ALDH1A1-siRNA or control-siRNA for 48 h, and then further treated with 1 μ M GEM for 48 h. (A) Western blot analysis for the detection of poly(ADP-ribose) polymerase (PARP) cleavage was used to determine apoptotic cell death. (B) A caspase-3 activity assay for the detection of caspase-3 activity was also used to determine apoptotic cell death. Error bars represent the standard deviation. ** P

    Techniques Used: Small Interfering RNA, Transfection, Western Blot, Caspase-3 Activity Assay, Activity Assay, Standard Deviation

    Aldehyde dehydrogenase 1A1 (ALDH1A1) knockdown enhances apoptotic cell death by gemcitabine (GEM). MIA PaCa-2 cells were transfected with ALDH1A1-siRNA or control-siRNA for 48 h, and then further treated with 1 μ M GEM for 48 h. (A) Western blot analysis for the detection of poly(ADP-ribose) polymerase (PARP) cleavage was used to measure apoptotic cell death. Anti-α-tubulin antibody was used for loading and transfer control. (B) A caspase-3 activity assay was also used to determine apoptotic cell death. Error bars represent the standard deviation. *** P
    Figure Legend Snippet: Aldehyde dehydrogenase 1A1 (ALDH1A1) knockdown enhances apoptotic cell death by gemcitabine (GEM). MIA PaCa-2 cells were transfected with ALDH1A1-siRNA or control-siRNA for 48 h, and then further treated with 1 μ M GEM for 48 h. (A) Western blot analysis for the detection of poly(ADP-ribose) polymerase (PARP) cleavage was used to measure apoptotic cell death. Anti-α-tubulin antibody was used for loading and transfer control. (B) A caspase-3 activity assay was also used to determine apoptotic cell death. Error bars represent the standard deviation. *** P

    Techniques Used: Transfection, Western Blot, Caspase-3 Activity Assay, Standard Deviation

    13) Product Images from "Self-assembled glycopeptide nanofibers as modulators of galectin-1 bioactivity"

    Article Title: Self-assembled glycopeptide nanofibers as modulators of galectin-1 bioactivity

    Journal: Cellular and molecular bioengineering

    doi: 10.1007/s12195-015-0399-2

    LacNAc-Q11 nanofibers inhibited galectin-1-mediated Jurkat T cell agglutination and apoptosis a) Cytotoxicity of GlcNAc-Q11 and LacNAc-Q11 nanofibers. b) Bright-field photomicrographs of Jurkat T cell agglutination in culture media with or without Gal-1 in the presence of 1 mM Q11 nanofibers with 0, 100, or 250 μM LacNAc, or 250 μM β-lactose. c) Bright-field and fluorescent photomicrographs of Jurkat T cells stained with Annexin V-eGFP to assess phosphatidylserine exposure, an early marker of apoptosis, in media supplemented with 10 μM Gal-1 alone or with 1 mM Q11 nanofibers having 100 μM LacNAc-Q11. d) Metabolic activity of Jurkat T cells in media supplemented with 10 μM Gal-1 plus 1 mM Q11 nanofibers with 0-250 μM LacNAc or 500 μM thiodigalactoside, a known galectin inhibitor and stable analog of β-lactose. * represents p
    Figure Legend Snippet: LacNAc-Q11 nanofibers inhibited galectin-1-mediated Jurkat T cell agglutination and apoptosis a) Cytotoxicity of GlcNAc-Q11 and LacNAc-Q11 nanofibers. b) Bright-field photomicrographs of Jurkat T cell agglutination in culture media with or without Gal-1 in the presence of 1 mM Q11 nanofibers with 0, 100, or 250 μM LacNAc, or 250 μM β-lactose. c) Bright-field and fluorescent photomicrographs of Jurkat T cells stained with Annexin V-eGFP to assess phosphatidylserine exposure, an early marker of apoptosis, in media supplemented with 10 μM Gal-1 alone or with 1 mM Q11 nanofibers having 100 μM LacNAc-Q11. d) Metabolic activity of Jurkat T cells in media supplemented with 10 μM Gal-1 plus 1 mM Q11 nanofibers with 0-250 μM LacNAc or 500 μM thiodigalactoside, a known galectin inhibitor and stable analog of β-lactose. * represents p

    Techniques Used: Agglutination, Staining, Marker, Activity Assay

    Related Articles

    Colorimetric Assay:

    Article Title: Intracellular Glutathione Depletion by Oridonin Leads to Apoptosis in Hepatic Stellate Cells
    Article Snippet: .. Z-DEVD-fmk and the Caspase 3/CPP32 colorimetric assay kit were obtained from BioVision (Mountain, PA, USA). .. The cell proliferation reagent WST-1 and RNase A were obtained from Roche Applied Sciences (Mannheim, Germany).

    MTT Assay:

    Article Title: Lobocrassin B Induces Apoptosis of Human Lung Cancer and Inhibits Tumor Xenograft Growth
    Article Snippet: .. Caspase Inhibitor Assay The cells were seeded into 24-well plates at 2 × 104 cells/well and were grown overnight and then pre-treated with inhibitors of caspase-3 (Z-DEVD-FMK, 50 µM, BioVision, San Francisco, CA, USA), caspase-8 (Z-IETD-FMK; 50 µM, San Francisco, CA, USA), and caspase-9 (Z-LEHD-FMK; 50 µM, San Francisco, CA, USA) for 2 h. Cells were then treated with 5 μM Lobocrassin B for 24 h. The cell viability were examined by MTT assay. .. DNA Content by Flow Cytometric Analysis Cells (2 × 105 /well) were subcultured into 6-well plates and treated with 1.25 and 2.5, 5 μM Lobocrassin B for 24 h. After challenge, cells were harvested by trypsinization and washed with PBS, fixed in 70% (v /v ) ethanol at −20 °C overnight.

    Mouse Assay:

    Article Title: A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells
    Article Snippet: .. Casp3Inh (Z-DEVD-FMK, 10 mM; BioVision Inc.) dissolved in DMSO was injected to 12-week-old C3H mice (The Jackson Laboratory) intraperitoneally at a dosage of 0.4 μl/g body weight twice a week for 2 weeks starting 2 days after ovariectomy. .. An equal volume of DMSO was injected as a control.

    Incubation:

    Article Title: Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells
    Article Snippet: .. Recombinant Pax7 protein (100–300 ng) and recombinant active-caspase 3 (0.5 µg; Chemicon) were incubated for 3 h in cleavage assay buffer [50 mM Hepes, pH 7.5; 0.1 M NaCl; 10% (vol/vol) glycerol; 0.1% Chaps; 10 mM DTT] containing either DMSO or z.DEVD.fmk (20 µM; BioVision) as indicated. ..

    other:

    Article Title: Caffeine induces sustained apoptosis of human gastric cancer cells by activating the caspase-9/caspase-3 signalling pathway
    Article Snippet: Two inhibitors, Z-LEHD-FMK (a specific inhibitor of caspase-9) and Z-DEVD-FMK (a specific inhibitor of caspase-3), were obtained from BioVision, Inc. (Milpitas, CA, USA).

    Injection:

    Article Title: A crucial role of caspase-3 in osteogenic differentiation of bone marrow stromal stem cells
    Article Snippet: .. Casp3Inh (Z-DEVD-FMK, 10 mM; BioVision Inc.) dissolved in DMSO was injected to 12-week-old C3H mice (The Jackson Laboratory) intraperitoneally at a dosage of 0.4 μl/g body weight twice a week for 2 weeks starting 2 days after ovariectomy. .. An equal volume of DMSO was injected as a control.

    Recombinant:

    Article Title: Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells
    Article Snippet: .. Recombinant Pax7 protein (100–300 ng) and recombinant active-caspase 3 (0.5 µg; Chemicon) were incubated for 3 h in cleavage assay buffer [50 mM Hepes, pH 7.5; 0.1 M NaCl; 10% (vol/vol) glycerol; 0.1% Chaps; 10 mM DTT] containing either DMSO or z.DEVD.fmk (20 µM; BioVision) as indicated. ..

    Cleavage Assay:

    Article Title: Caspase 3 cleavage of Pax7 inhibits self-renewal of satellite cells
    Article Snippet: .. Recombinant Pax7 protein (100–300 ng) and recombinant active-caspase 3 (0.5 µg; Chemicon) were incubated for 3 h in cleavage assay buffer [50 mM Hepes, pH 7.5; 0.1 M NaCl; 10% (vol/vol) glycerol; 0.1% Chaps; 10 mM DTT] containing either DMSO or z.DEVD.fmk (20 µM; BioVision) as indicated. ..

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    BioVision m dtt
    M Dtt, supplied by BioVision, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/BioVision
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
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