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Abcam m dtt
M Dtt, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/Abcam
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
99/100 stars

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Concentration Assay:

Article Title: MicroRNA-145 Regulates Human Corneal Epithelial Differentiation
Article Snippet: .. The clear supernatant was collected for protein denaturation in a buffer at a final concentration of 2% sodium dodecylsulfate (SDS), 50 mM DL-dithiothreitol and 1% glycerol and analyzed by 10% SDS-PAGE (polyacrylamide gel electrophoresis) using monoclonal antibody against Cnx-43, ABCG2 (Abcam), p63α (Cell Signaling, Danvers, MA, US) or GAPDH (Sigma), followed by appropriate horseradish peroxidase-conjugated Ig secondary antibodies. .. Staining signals were detected by enhanced chemiluminescence (ECL, GE Healthcare).

Article Title: Assembly and Folding Properties of Cytosolic IgG Intrabodies
Article Snippet: .. To validate the complete removal of DTT in the above column and dialysis steps, 100 mM DTT in PBS was passed through the desalting column then subjected to dialysis against PBS, and the concentration of DTT was determined using a free thiol detection kit (Abcam; cat# ab112158). ..

Incubation:

Article Title: Activation and cleavage of SASH1 by caspase-3 mediates an apoptotic response
Article Snippet: .. SASH1-bound beads were incubated for 16 h in protease inhibitor-free buffer (25 mM HEPES, 0.1% (w/v) CHAPS, 10 mM DTT, pH 7.5) with and without 2 U of active recombinant caspase-3 (Abcam, Melbourne, VIC, Australia). ..

Activity Assay:

Article Title: Calcium Channel Blockade Ameliorates Endoplasmic Reticulum Stress in the Hippocampus Induced by Amyloidopathy in the Entorhinal Cortex
Article Snippet: .. PDI assayPDI activity was determined by measuring reduction of insulin in the presence of dithiothreitol (DTT) as a reducing agent. ..

Polyacrylamide Gel Electrophoresis:

Article Title: MicroRNA-145 Regulates Human Corneal Epithelial Differentiation
Article Snippet: .. The clear supernatant was collected for protein denaturation in a buffer at a final concentration of 2% sodium dodecylsulfate (SDS), 50 mM DL-dithiothreitol and 1% glycerol and analyzed by 10% SDS-PAGE (polyacrylamide gel electrophoresis) using monoclonal antibody against Cnx-43, ABCG2 (Abcam), p63α (Cell Signaling, Danvers, MA, US) or GAPDH (Sigma), followed by appropriate horseradish peroxidase-conjugated Ig secondary antibodies. .. Staining signals were detected by enhanced chemiluminescence (ECL, GE Healthcare).

Staining:

Article Title: Systematic analysis of the IL‐17 receptor signalosome reveals a robust regulatory feedback loop
Article Snippet: .. In order to check the production of SF‐IL‐17, samples were mixed with SDS sample buffer, reduced with 50 mM dithiothreitol (DTT) or left untreated, separated using SDS‐PAGE and gels were stained with InstantBlue Coomassie protein stain (Expedeon). .. In order to test functionality of produced SF‐IL‐17, cells were incubated on ice with SF‐IL‐17 (1 μg/ml) in FACS buffer (PBS/0.2%FCS/0.1%NaN3) and washed and binding of SF‐IL‐17 was detected using the APC‐conjugated anti‐Flag antibody on Accuri C6 cytometer (BD Biosciences) and analyzed by FlowJo (BD Biosciences).

Recombinant:

Article Title: Activation and cleavage of SASH1 by caspase-3 mediates an apoptotic response
Article Snippet: .. SASH1-bound beads were incubated for 16 h in protease inhibitor-free buffer (25 mM HEPES, 0.1% (w/v) CHAPS, 10 mM DTT, pH 7.5) with and without 2 U of active recombinant caspase-3 (Abcam, Melbourne, VIC, Australia). ..

SDS Page:

Article Title: Systematic analysis of the IL‐17 receptor signalosome reveals a robust regulatory feedback loop
Article Snippet: .. In order to check the production of SF‐IL‐17, samples were mixed with SDS sample buffer, reduced with 50 mM dithiothreitol (DTT) or left untreated, separated using SDS‐PAGE and gels were stained with InstantBlue Coomassie protein stain (Expedeon). .. In order to test functionality of produced SF‐IL‐17, cells were incubated on ice with SF‐IL‐17 (1 μg/ml) in FACS buffer (PBS/0.2%FCS/0.1%NaN3) and washed and binding of SF‐IL‐17 was detected using the APC‐conjugated anti‐Flag antibody on Accuri C6 cytometer (BD Biosciences) and analyzed by FlowJo (BD Biosciences).

Article Title: MicroRNA-145 Regulates Human Corneal Epithelial Differentiation
Article Snippet: .. The clear supernatant was collected for protein denaturation in a buffer at a final concentration of 2% sodium dodecylsulfate (SDS), 50 mM DL-dithiothreitol and 1% glycerol and analyzed by 10% SDS-PAGE (polyacrylamide gel electrophoresis) using monoclonal antibody against Cnx-43, ABCG2 (Abcam), p63α (Cell Signaling, Danvers, MA, US) or GAPDH (Sigma), followed by appropriate horseradish peroxidase-conjugated Ig secondary antibodies. .. Staining signals were detected by enhanced chemiluminescence (ECL, GE Healthcare).

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    Abcam m dtt
    M Dtt, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m dtt/product/Abcam
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m dtt - by Bioz Stars, 2020-09
    92/100 stars
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