Structured Review

ATUM m dtt
M Dtt, supplied by ATUM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m dtt/product/ATUM
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m dtt - by Bioz Stars, 2020-09
90/100 stars

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Incubation:

Article Title: CerS6 Is a Novel Transcriptional Target of p53 Protein Activated by Non-genotoxic Stress *
Article Snippet: .. DNA and p53 were incubated in the reaction mixture containing 2 μl of 10× buffer (100 m m Tris, 500 m m KCl, 10 m m DTT, pH 7.5), 1 μl of poly(dI·dC) (1 μg/μl in 10 m m Tris-HCl, 1 m m EDTA, pH 7.5), 2 μl of 25 m m DTT, 1 μl of the corresponding DNA (20 pmol/μl) in 1× Tris-EDTA buffer, 2 μl of purified p53 protein (5 ng/μl in 20 m m Tris-HCl buffer, pH 7.5), and 12 μl of H2 O. .. Reaction mixtures were incubated for 30 min at room temperature.

Purification:

Article Title: CerS6 Is a Novel Transcriptional Target of p53 Protein Activated by Non-genotoxic Stress *
Article Snippet: .. DNA and p53 were incubated in the reaction mixture containing 2 μl of 10× buffer (100 m m Tris, 500 m m KCl, 10 m m DTT, pH 7.5), 1 μl of poly(dI·dC) (1 μg/μl in 10 m m Tris-HCl, 1 m m EDTA, pH 7.5), 2 μl of 25 m m DTT, 1 μl of the corresponding DNA (20 pmol/μl) in 1× Tris-EDTA buffer, 2 μl of purified p53 protein (5 ng/μl in 20 m m Tris-HCl buffer, pH 7.5), and 12 μl of H2 O. .. Reaction mixtures were incubated for 30 min at room temperature.