m br utp (Thermo Fisher)

Thermo Fisher is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Name:

RNaseOUT Recombinant Ribonuclease Inhibitor

Description:

RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C

Catalog Number:

10777019

Price:

None

Applications:

PCR & Real-Time PCR|Reverse Transcription

Category:

Proteins Enzymes Peptides

Buy from Supplier

Structured Review

Thermo Fisher m br utp
RNaseOUT Recombinant Ribonuclease Inhibitor is a potent noncompetitive inhibitor of pancreatic type ribonucleases such as RNase A and is used to avoid RNA degradation in a variety of applications RNaseOUT Recombinant Ribonuclease Inhibitor is an acidic protein with a molecular weight of 52 kDa RNaseOUT inhibits RNase A RNase B and RNase C ApplicationscDNA synthesis RT PCR and in vitro transcription and translationSourcePurified by affinity chromatography from E coli expressing a cloned porcine genePerformance and quality testingSDS PAGE purity endodeoxyribonuclease assay protein concentration specific activity performance evaluated by RT PCRUnit definitionOne unit inhibits 5 ng of RNase A by 50 using cytidine 2 3 cyclic monophosphate cCMP as the substrateUnit reaction conditions100 mM Tris acetate pH 6 5 1 mM EDTA 0 2 mM cCMP 2 µg RNase A in 1 mL from 0 to 10 min at 25°C
https://www.bioz.com/result/m br utp/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99

m br utp - by Bioz Stars, 2021-01

99/100 stars

Related Products / Commonly Used Together

gtp
rnaseout
ctp
m atp
run-on buffer

Images

Sample Prep:

Article Title: FACS purification of Drosophila larval Neuroblasts for next generation sequencing
Article Snippet: .. Chloroform/Trichlormethan (Roth, cat. no. 3313.4) Isopropanol (Roth, cat. no. CP41) RNase free water (Life technologies, cat. no. AM9932) Diethylpyrocarbonate (DEPC) treated water (Life technologies, cat. no. 750023) Glycoblue (Life technologies, cat. no. AM9515) Agilent RNA 6000 Nano Kit (Agilent Technologies, cat. no. 5067-1511) Agilent RNA 6000 Pico Kit (Agilent Technologies, cat. no. 5067-1513) Dynabeads mRNA purification Kit (Life technologies, cat. no. 61006) Fragmentation buffer 5× (see REAGENT SETUP) Tris base, adjusted to pH 8.2 (Sigma-Aldrich, cat. no. T1503) Potassium acetate (Sigma-Aldrich, cat. no. P1190) Magensium acetate (Sigma-Aldrich, cat. no. M5661) Sodium Acetate 3 M, pH 5.5 (Life technologies, cat. no. AM9740) Random hexamer primers 3 μg/μL (Life technologies, cat. no. 48190-011) dNTP Mix, 10 mM (Life technologies, cat. no. 18427-013) SuperScript® II Reverse Transcriptase (Life technologies, cat. no. 18064-014) SuperScript® III Reverse Transcriptase, in a set containing 5× first-strand buffer and 0.1 M DTT (Life technologies, cat. no. 18080-044) iQ SYBR Green Supermix (Bio-Rad, cat. no. 170-8882) RNaseOUT™ (Life technologies, cat. no. 10777-019) Second-Strand Buffer (Life technologies, cat. no. 10812-014) dUTP (Fermentas, cat. no. R0133) dATP (Fermentas, cat. no. R0141) dCTP (Fermentas, cat. no. R0151) dGTP (Fermentas, cat. no. R0161) DNA Polymerase I ( Escherichia coli ) (Life technologies, cat. no. 18010-017) Ribonuclease H (Life technologies, cat. no. 18021-014) E. coli DNA Ligase (Life technologies, cat. no. 18052-019) MinElute Reaction Cleanup Kit (QIAGEN, cat. no. 28204) Quant-iT Picogreen dsDNA Assay Kit (Life technologies, cat. no. P7589) NEBNext DNA sample Prep Reagent kits (New England Biolabs, cat. no. E6040) .. Foreceps Dumont Nr.5 (Fine Science Tools, cat. no. 11252-23) Stereomicrosope SteREO Discovery.V8 (Zeiss) Glass-bottom dishes, 35mm (MatTek Corporation, cat. no. P35G-0-14-C) Steriflip (Millipore, cat. no. SCGP00525) Thermo mixer compact (Eppendorf) Cell strainer, 35 μm (BD Bioscience Falcon, cat. no. 352235) FACSAriaIII, cell sorter (BD Bioscience) Low-binding reagent tubes, 1.5 mL (Life technologies, cat. no. AM12450) Phase-lock heavy gel tubes, 2 mL (5 PRIME, cat. no. 2302830) Agilent 2100 Bioanalyzer (Agilent Technologies) Magnetic rack (for example DynaMag™-2, Life technologies, cat. no. 12321D) NanoDrop 3300 fluorospectrophotometer (Thermo Scientific) Genome Analyzer IIX (GAIIX; Illumina) HiSeq 2000 sequence analyzer (Illumina)

In Vitro:

Article Title: Design and application of cotranscriptional non-enzymatic RNA circuits and signal transducers
Article Snippet: .. In vitro transcription In all, 100 pg to 1000 ng of double-stranded DNA transcription templates was transcribed using 100 U of T7 RNA polymerase (NEB) in 50 µl reactions containing 40 mM Tris–HCl, pH 7.9, 30 mM MgCl2 , 10 mM DTT, 2 mM spermidine, 4 mM ribonucleotide (rNTP) mix and 20 U of the recombinant ribonuclease inhibitor RNaseOUT (Life Technologies). .. Transcription reactions were incubated at 42°C for 1–2 h. After this, transcripts of the circuit components were either (i) used directly for assembly or (ii) subjected to purification before assembly.

Serial Dilution:

Article Title: A comparative study of digital PCR and real-time qPCR for the detection and quantification of HPV mRNA in sentinel lymph nodes of cervical cancer patients
Article Snippet: .. For cDNA synthesis of clinical samples as well as for the serial dilution series, 5 µg of total RNA were reverse transcribed in a 80 μl reaction comprising 40 pmol CDS-primer (5′-Tn= 30 VN-3′), 40 pmol of each dNTP, first strand buffer, 5 mM DTT, 80 U RNaseOUT™ (Thermo Fisher Scientific, Catalog Number 10777019) and 400 U SuperScriptII (Thermo Fisher Scientific, Catalog Number 18064014). .. To allow optimal annealing, primer and RNA were incubated at 65 °C for 5 min and cooled down on ice.

Picogreen Assay:

Labeling:

Article Title: L1 retrotransposition is activated by Ten-eleven-translocation protein 1 and repressed by methyl-CpG binding proteins
Article Snippet: .. 500 ng of total RNA were used for cDNA synthesis using 200 units M-MuLV reverse transcriptase (NEB), 0.01 OD units random primer from the Prime-It II Random Primer Labeling Kit (Stratagene), 0.5 mM dNTPs (Carl Roth GmbH) and 40 units recombinant ribonuclease inhibitor RNaseOUT (Invitrogen) in a total reaction volume of 20 µL. .. For qPCR, 0.5 ng of cDNA were used for each reaction.

Purification:

SYBR Green Assay:

Random Hexamer Labeling:

Recombinant:

Article Title: Splitomicin, a SIRT1 Inhibitor, Enhances Hematopoietic Differentiation of Mouse Embryonic Stem Cells
Article Snippet: .. Then, 2 μ g of the total RNA was reverse-transcribed in the first-strand buffer containing 6 μ g/ml oligo (dT) primer, 50 U M-MLV reverse transcriptase (Invitrogen), 2 mM dNTP, and 40 U RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). .. One microliter of the cDNA synthesis was subjected to a standard PCR reaction of 20~35 cycles of denaturation for 60 seconds at 95°C, annealing for 60 seconds at 58~60°C, and elongation for 60 seconds at 72°C.

Article Title: Genome-wide gene expression analysis of anguillid herpesvirus 1
Article Snippet: .. RNA was extracted according to manufacturer’s protocol, and dissolved in 200 μl RNase-free water (Sigma-Aldrich) with 2 μl RNaseOUT recombinant ribonuclease inhibitor (Invitrogen). .. RNA concentration was checked by spectrophotometry using a Nanodrop ND-1000 (Thermo Fisher Scientific, MA, USA).