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Cell Signaling Technology Inc m β glycerophosphate
M β Glycerophosphate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m β glycerophosphate/product/Cell Signaling Technology Inc
Average 92 stars, based on 4 article reviews
Price from $9.99 to $1999.99
m β glycerophosphate - by Bioz Stars, 2020-09
92/100 stars

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Protease Inhibitor:

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

Article Title: Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration *
Article Snippet: .. 450 μl of modified NEB buffer ( ) (50 m m HEPES-KOH (pH 7.4), 5 m m MnCl2 , 10 m m MgCl2 , 5 m m EGTA, 2 m m EDTA, 100 m m NaCl, 5 m m KCl, 0.1% (v/v) Nonidet P-40, 30 μg/ml RNase A, 30 μg/ml DNase I, 1 m m PMSF, 5 m m β-glycerophosphate, 5 m m NaF, 1× protease inhibitor mixture, and 1× phosphatase inhibitor mixture) was added, and Myc-NEK3 was immunoprecipitated using 1 μl of the anti-Myc antibody (Cell Signaling Technology, catalog no. 2276) with rotation overnight at 4 °C. .. Immune complexes were captured using 50 μl of recombinant protein G-agarose beads (50% slurry in PBS) with rotation at 4 °C for 2 h. Beads were subsequently washed three times in modified NEB buffer and twice in kinase wash buffer (50 m m HEPES-KOH (pH 7.4) and 5 m m MnCl2 ).

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

Generated:

Article Title: Proteomic Profiling of the Planarian Schmidtea mediterranea and Its Mucous Reveals Similarities with Human Secretions and Those Predicted for Parasitic Flatworms *
Article Snippet: .. Lysates were generated from whole organisms of the CIW4 clonal strain of asexual Schmidtea mediterranea , sized matched to 2–4 mm, using a tissue homogenizer and lysis buffer containing 20 m m HEPES buffer (Cell Signaling Technology, Boston, MA) 8 m urea (EMD Chemicals, Darmstadt, Germany), 1 m m sodium orthovanadate (BioShop Canada Inc., Burlington, ON, Canada), 2.5 m m sodium pyrophosphate (Cell Signaling Technology), and 1 m m β-glycerophosphate (Cell Signaling Technology). .. Whole organisms were also treated with 5% NAC (Sigma-Aldrich, St. Louis, MO) in 1× PBS (BioShop) for 8 min to remove their mucous coating , and subsequently lysed as described above.

Immunoprecipitation:

Article Title: Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration *
Article Snippet: .. 450 μl of modified NEB buffer ( ) (50 m m HEPES-KOH (pH 7.4), 5 m m MnCl2 , 10 m m MgCl2 , 5 m m EGTA, 2 m m EDTA, 100 m m NaCl, 5 m m KCl, 0.1% (v/v) Nonidet P-40, 30 μg/ml RNase A, 30 μg/ml DNase I, 1 m m PMSF, 5 m m β-glycerophosphate, 5 m m NaF, 1× protease inhibitor mixture, and 1× phosphatase inhibitor mixture) was added, and Myc-NEK3 was immunoprecipitated using 1 μl of the anti-Myc antibody (Cell Signaling Technology, catalog no. 2276) with rotation overnight at 4 °C. .. Immune complexes were captured using 50 μl of recombinant protein G-agarose beads (50% slurry in PBS) with rotation at 4 °C for 2 h. Beads were subsequently washed three times in modified NEB buffer and twice in kinase wash buffer (50 m m HEPES-KOH (pH 7.4) and 5 m m MnCl2 ).

Article Title: Two Nedd4-binding Motifs Underlie Modulation of Sodium Channel Nav1.6 by p38 MAPK *
Article Snippet: .. Immunoprecipitated proteins bound to beads were used as substrates in a kinase reaction containing 20 m m MOPS, pH 7.2, 25 m m β-glycerophosphate, 5 m m EGTA, 1 m m Na3 VO4 , 1 m m dithiothreitol, 0.5% DMSO, 10 m m MgCl2 , 25 μ m ATP, 10 μCi [γ-32 P]ATP, and 10 ng/μl recombinant activated p38α (Cell Signaling). .. The active p38 MAPK inhibitor SB203580 (10 μ m ) was added to one reaction tube to confirm that the incorporation of radioactivity was not due to a contaminating kinase that may have co-precipitated with the CD4 fusion proteins.

Incubation:

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

Modification:

Article Title: Identification of NEK3 Kinase Threonine 165 as a Novel Regulatory Phosphorylation Site That Modulates Focal Adhesion Remodeling Necessary for Breast Cancer Cell Migration *
Article Snippet: .. 450 μl of modified NEB buffer ( ) (50 m m HEPES-KOH (pH 7.4), 5 m m MnCl2 , 10 m m MgCl2 , 5 m m EGTA, 2 m m EDTA, 100 m m NaCl, 5 m m KCl, 0.1% (v/v) Nonidet P-40, 30 μg/ml RNase A, 30 μg/ml DNase I, 1 m m PMSF, 5 m m β-glycerophosphate, 5 m m NaF, 1× protease inhibitor mixture, and 1× phosphatase inhibitor mixture) was added, and Myc-NEK3 was immunoprecipitated using 1 μl of the anti-Myc antibody (Cell Signaling Technology, catalog no. 2276) with rotation overnight at 4 °C. .. Immune complexes were captured using 50 μl of recombinant protein G-agarose beads (50% slurry in PBS) with rotation at 4 °C for 2 h. Beads were subsequently washed three times in modified NEB buffer and twice in kinase wash buffer (50 m m HEPES-KOH (pH 7.4) and 5 m m MnCl2 ).

Lysis:

Article Title: Disruption of Nongenomic Testosterone Signaling in a Model of Spinal and Bulbar Muscular Atrophy
Article Snippet: .. Cell lysates were prepared in lysis buffer [20 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m EGTA, 1 m m EDTA, 1% Triton X-100, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin; Cell Signaling] supplemented with protease inhibitors (Mini Complete; Roche, France). .. Lysates were centrifuged at 3000 × g for 10 min at 4 C, and protein concentrations of supernatants were determined by the BCA (bicinchoninic acid) method (Thermo Fisher Scientific, France).

Article Title: Fat-specific Protein 27 (FSP27) Interacts with Adipose Triglyceride Lipase (ATGL) to Regulate Lipolysis and Insulin Sensitivity in Human Adipocytes *
Article Snippet: .. Fully differentiated human adipocytes were harvested in 1 ml of lysis buffer containing 20 m m Tris (pH 7.5), 150 m m NaCl, 1% Triton X-100, 1 m m Na2 EDTA, 2.5 m m sodium pyrophosphate, 1 m m β-glycerophosphate, 1 m m Na3 VO4 , 1 μg/ml leupeptin (Cell Signaling Technology), phosphatase inhibitor mixture, and protease inhibitor mixture (Roche). .. Cleared lysates containing 1 mg of extracted protein were incubated with 50 μl of cross-linked ATGL, FLAG, or HA protein A/G-Sepharose beads or with protein A/G-Sepharose beads alone as a control overnight at 4 °C.

Article Title: NADPH:Quinone Oxidoreductase 1 Regulates Host Susceptibility to Ozone via Isoprostane Generation *
Article Snippet: .. Beads were washed twice with lysis buffer and then twice with kinase reaction buffer (25 m m Tris-HCl (pH 7.5), 5 m m β-glycerophosphate, 2 m m dithiothreitol (DTT), 0.1 m m Na3 VO4 , and 10 m m MgCl2 ) (Cell Signaling Technology). .. The IKK activity assay was initiated by the addition of kinase buffer containing 200 μ m ATP and 1 μg of GST-IκBα as a substrate for the IKK enzyme activity.

Article Title: Proteomic Profiling of the Planarian Schmidtea mediterranea and Its Mucous Reveals Similarities with Human Secretions and Those Predicted for Parasitic Flatworms *
Article Snippet: .. Lysates were generated from whole organisms of the CIW4 clonal strain of asexual Schmidtea mediterranea , sized matched to 2–4 mm, using a tissue homogenizer and lysis buffer containing 20 m m HEPES buffer (Cell Signaling Technology, Boston, MA) 8 m urea (EMD Chemicals, Darmstadt, Germany), 1 m m sodium orthovanadate (BioShop Canada Inc., Burlington, ON, Canada), 2.5 m m sodium pyrophosphate (Cell Signaling Technology), and 1 m m β-glycerophosphate (Cell Signaling Technology). .. Whole organisms were also treated with 5% NAC (Sigma-Aldrich, St. Louis, MO) in 1× PBS (BioShop) for 8 min to remove their mucous coating , and subsequently lysed as described above.

Article Title: Glucose Regulates Hypothalamic Long-chain Fatty Acid Metabolism via AMP-activated Kinase (AMPK) in Neurons and Astrocytes *
Article Snippet: .. Cortical and MBH slices were preincubated in aCSF containing 1 m m glucose and incubated in aCSF containing 1 or 10 m m glucose for 15 min. After washing with cold PBS, samples were lysed in ice-cold lysis buffer containing 1 m Tris-HCl, pH 7.5, 1 m NaCl, 400 m m Na2 EDTA, 100 m m EGTA, pH 7.5, Triton X-100, sodium pyrophosphate, 100 m m β-glycerophosphate, 100 m m Na3 VO4 , and 10 mg/ml leupeptin supplemented with 1% protease inhibitor mixture (Cell Signaling), 1 m m PMSF, and aprotonin. .. Total protein (20 μg) was resolved on a 7.5% acrylamide gel and transferred to a nitrocellulose membrane (Bio-Rad) blocked with 5% nonfat milk in Tris-buffered saline containing 0.2% Tween 20 for 1 h. Blots were then incubated with a P-AMPK or P-ACC primary antibody (1:1000, Cell Signaling) overnight at 4 °C followed by secondary antibody incubation (HRP-goat anti rabbit, 1:10,000, Bio-Rad) for 1 h at room temperature.

Recombinant:

Article Title: Two Nedd4-binding Motifs Underlie Modulation of Sodium Channel Nav1.6 by p38 MAPK *
Article Snippet: .. Immunoprecipitated proteins bound to beads were used as substrates in a kinase reaction containing 20 m m MOPS, pH 7.2, 25 m m β-glycerophosphate, 5 m m EGTA, 1 m m Na3 VO4 , 1 m m dithiothreitol, 0.5% DMSO, 10 m m MgCl2 , 25 μ m ATP, 10 μCi [γ-32 P]ATP, and 10 ng/μl recombinant activated p38α (Cell Signaling). .. The active p38 MAPK inhibitor SB203580 (10 μ m ) was added to one reaction tube to confirm that the incorporation of radioactivity was not due to a contaminating kinase that may have co-precipitated with the CD4 fusion proteins.

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    Cell Signaling Technology Inc m β glycerophosphate
    M β Glycerophosphate, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m β glycerophosphate/product/Cell Signaling Technology Inc
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m β glycerophosphate - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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