lysosome isolation kit  (Millipore)


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    Name:
    Lysosome Isolation Kit
    Description:
    Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells They contain many acid hydrolases that take part in protein degradation in the cell Lysosomes also contain lipases nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs Gaucher Hunter disease and others They also contribute to maintaining cellular homeostasis Malfunctions in this organelle directly impact cell behavior and fate Lysosomes may also be involved in other cellular processes such as Albinism and aging
    Catalog Number:
    lysiso1
    Price:
    None
    Applications:
    Lysosome Isolation Kit has been used:. in the isolation of cytosol and lysosome. to obtain enriched light and heavy lysosomal fractions from cultured cells. in the isolation of lysosomes of mouse bone marrow macrophages (BMMs). in lysosome purification
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    Structured Review

    Millipore lysosome isolation kit
    Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells They contain many acid hydrolases that take part in protein degradation in the cell Lysosomes also contain lipases nucleases and polysaccharidases and deficiencies in some of these enzymes lead to specific lysosomal storage diseases such as Tay Sachs Gaucher Hunter disease and others They also contribute to maintaining cellular homeostasis Malfunctions in this organelle directly impact cell behavior and fate Lysosomes may also be involved in other cellular processes such as Albinism and aging
    https://www.bioz.com/result/lysosome isolation kit/product/Millipore
    Average 99 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    lysosome isolation kit - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    MTT Assay:

    Article Title: Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion
    Article Snippet: .. 4′, 6-Diamidino-2-phenylindole dihydrochloride (DAPI), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Lipopolysaccharides (LPS, L3024), lysosome isolation kit, bicinchoninic acid (BCA) protein assay kit and phospholipid assay kit were obtained from Sigma-Aldrich (USA). .. Interferon γ (IFN-γ, 315-05) was obtained from PeproTech (USA).

    Article Title: trans-4,4’-Dihydroxystilbene (DHS) inhibits human neuroblastoma tumor growth and induces mitochondrial and lysosomal damages in neuroblastoma cell lines
    Article Snippet: .. Penicillin, streptomycin, phenylmethylsulfonyl fluoride (PMSF), aprotinin, sodium orthovanadate (NaVO4 ), cis-platin®, acridine orange (AO), Triton X-100, propidium iodide (PI), leupeptin (Leu) and pepstatin A (Pep A), RNAse A, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), uridine, sodium pyruvate, N-acetylcysteine (NAC), ethidium bromide (EtBr), annexin-V kit, JC-1, 2’,7’-dichlorodihydrofluorescin diacetate (H2DCFDA), LYSISO1 kit, and antibodies for pro-caspases-3, -8, -9 were procured from Sigma chemicals (St. Luois, MO). .. Other chemicals used were: Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Lysotracker Red (Life Technologies, Carlsbad, CA), Hoechst 33342 (Molecular Probes, Inc., Eugene, OR), Z-LEHD-FMK, Z-IETD-FMK, Z-DEVD-FMK, Z-VAD-FMK, U0126, SB203580 and SP600125 (Calbiochem, Gibbstown, NJ), antibodies for cytochrome c, cytochrome oxidase I (COX I), cytochrome oxidase IV (COX IV) and β-actin (Abcam Danvers, MA), for BAX, BCL-2, BID, t-BID, apoptosis-activating factor-1 (ApaF1), phosophorylated and non-phoshorylated -p38, -JNK, -ERK, cleaved caspases-3, -8, and -9 (Cell Signaling Technology Inc., Danvers, MA), for cathepsins-B, -L and -D (Novus Biologicals, Littleton, CO) and for LAMP1 (Santa Cruz Biotechnology Inc., Dallas, TX) and lipofectamine reagent (Invitrogen, Carlsbad, CA).

    Transfection:

    Article Title: PIP4K2A regulates intracellular cholesterol transport through modulating PI(4,5)P2 homeostasis
    Article Snippet: .. Peroxisomes and lysosomes from SV589 cells transfected with indicated siRNAs were purified with either a peroxisome or lysosome isolation kit (Sigma Aldrich) according to the manu­facturer’s instructions. ..

    Luciferase:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Isolation:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Article Title: Chaperone-mediated autophagy prevents apoptosis by degrading BBC3/PUMA
    Article Snippet: .. Livers were removed, washed with cold PBS, and homogenized in the extraction buffer (Sigma, lysosomal isolation kit, LYSISO1-1KT). .. After separation by density gradient centrifugation (150,000 × g for 4 h), lysosomes were isolated from lysosomal fraction and tested for LAMP2A levels by immunoblotting.

    Article Title: Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion
    Article Snippet: .. 4′, 6-Diamidino-2-phenylindole dihydrochloride (DAPI), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Lipopolysaccharides (LPS, L3024), lysosome isolation kit, bicinchoninic acid (BCA) protein assay kit and phospholipid assay kit were obtained from Sigma-Aldrich (USA). .. Interferon γ (IFN-γ, 315-05) was obtained from PeproTech (USA).

    Article Title: PIP4K2A regulates intracellular cholesterol transport through modulating PI(4,5)P2 homeostasis
    Article Snippet: .. Peroxisomes and lysosomes from SV589 cells transfected with indicated siRNAs were purified with either a peroxisome or lysosome isolation kit (Sigma Aldrich) according to the manu­facturer’s instructions. ..

    Article Title: Disruption of chaperone-mediated autophagy-dependent degradation of MEF2A by oxidative stress-induced lysosome destabilization
    Article Snippet: .. Anti-LC3B (L7543), anti-LAMP1 (L1418), N -acetyl-Leu-Leu-Nle-CHO (ALLN, A6185), lactacystin (L6785), ammonium chloride (A0171), chloroquine diphosphate salt (C6628), 3-methyladenine (M9281), 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF, A8456), EZview™ Red Anti-c-MYC Affinity Gel, lysosomal isolation kit (LYSISO1) and acridine orange (318337) were purchased from Sigma Aldrich. .. Anti-HSPA8 (HSC70, ab51052) and anti-LAMP2A (ab18528) was purchased from Abcam.

    Article Title: Tumor Necrosis Factor-related Apoptosis-inducing Ligand (TRAIL) Protein-induced Lysosomal Translocation of Proapoptotic Effectors Is Mediated by Phosphofurin Acidic Cluster Sorting Protein-2 (PACS-2) *
    Article Snippet: .. Lysosomes were isolated from cell cultures using a commercially available lysosome isolation kit (Sigma Aldrich). .. Whole cell lysates were obtained by incubating cells for 30 min on ice with lysis buffer (50 m m Tris-HCl (pH 7.4); 1% Nonidet P-40; 0.25% sodium deoxycholate; 150 m m NaCl; 1 m m EDTA; 1 m m PMSF; 1 μg/ml aprotinin, leupeptin, and pepstatin; 1 m m Na3 VO4 ; 1 m m NaF) followed by centrifugation at 14,000 × g for 15 min at 4 °C.

    Bicinchoninic Acid Protein Assay:

    Article Title: Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion
    Article Snippet: .. 4′, 6-Diamidino-2-phenylindole dihydrochloride (DAPI), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Lipopolysaccharides (LPS, L3024), lysosome isolation kit, bicinchoninic acid (BCA) protein assay kit and phospholipid assay kit were obtained from Sigma-Aldrich (USA). .. Interferon γ (IFN-γ, 315-05) was obtained from PeproTech (USA).

    Centrifugation:

    Article Title: Elaiophylin, a novel autophagy inhibitor, exerts antitumor activity as a single agent in ovarian cancer cells
    Article Snippet: .. Lysosomal fractions were extracted from cell homogenates by differential centrifugation followed by density centrifugation according to the manufacturer's protocol (Lysosome Extraction Kit; Sigma-Aldrich; LYSISO1). ..

    Purification:

    Article Title: PIP4K2A regulates intracellular cholesterol transport through modulating PI(4,5)P2 homeostasis
    Article Snippet: .. Peroxisomes and lysosomes from SV589 cells transfected with indicated siRNAs were purified with either a peroxisome or lysosome isolation kit (Sigma Aldrich) according to the manu­facturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The RNA-binding protein Mex3B is a coreceptor of Toll-like receptor 3 in innate antiviral response
    Article Snippet: .. Reagents and antibodies Poly(I:C), LPS, R837, PGN, chloroquine (Invivogen); TNFα, IL-1β (R & D Systems); D-galactosamine (Sigma); GM-CSF (peproTech); ELISA kit for murine IFN-β, TNFα, IL-6 (Biolegend); dual-specific luciferase assay kit (Promega); EZ-link Psoralen-PEG3 -Biotin (Thermo); streptavidin agarose (Solulink); lysosome isolation kit (sigma); mouse monoclonal antibodies against Flag, β-actin (Sigma), HA (OriGene), phospho-IBα (Cell Signaling Technology), TLR3 (IMGENEX); rabbit monoclonal antibodies against TLR3, phospho-IRF3 (Cell Signaling Technology); rabbit polyclonal antibodies against IRF3 (Santa Cruz Biotechnology), TRIF (Cell Signaling Technology), TBK1 and phospho-TBK1(EPITOMICS), Flag (MBL); Alexa Fluor 555-conjugated goat anti-rabbit IgG antibody, Alexa Fluor 647-conjugated goat anti-mouse IgG antibody (Invitrogen) were purchased from the indicated manufacturers. .. Mouse antisera against human Mex3B were raised against recombinant human Mex3B fragments containing aa236-517 and aa1-517, respectively.

    Phospholipid Assay:

    Article Title: Proinflammatory macrophage-derived microvesicles exhibit tumor tropism dependent on CCL2/CCR2 signaling axis and promote drug delivery via SNARE-mediated membrane fusion
    Article Snippet: .. 4′, 6-Diamidino-2-phenylindole dihydrochloride (DAPI), dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), Lipopolysaccharides (LPS, L3024), lysosome isolation kit, bicinchoninic acid (BCA) protein assay kit and phospholipid assay kit were obtained from Sigma-Aldrich (USA). .. Interferon γ (IFN-γ, 315-05) was obtained from PeproTech (USA).

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  • 99
    Millipore lysosome isolation kit
    Lysosome Isolation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysosome isolation kit/product/Millipore
    Average 99 stars, based on 82 article reviews
    Price from $9.99 to $1999.99
    lysosome isolation kit - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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