lysosome isolation kit  (Millipore)


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    Millipore lysosome isolation kit
    Lysosome Isolation Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysosome isolation kit/product/Millipore
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    lysosome isolation kit - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: .. The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Article Title: Ubiquitination of Rheb governs growth factor-induced mTORC1 activation
    Article Snippet: Lysosomes isolation Lysosomes were isolated with lysosome Isolation Kit (Catalog Number LYSISO1, sigma), all subsequent steps of the lysosomal isolation were performed according to manufacturer’s description. .. After 20 strokes, the nuclei were removed by centrifugation at 1000×g for 10 min, the supernatant was centrifuged at 20,000×g for 20 min and the resulting pellet, containing the crude lysosomal fraction (CLF).

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosome Isolation Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

    Article Title: Eucommia ulmoides Leaf Extract Ameliorates Steatosis Induced by High-fat Diet in Rats by Increasing Lysosomal Function
    Article Snippet: Lysosomal Fractionation Lysosomal fractionation was conducted using the Lysosome Isolation Kit (Sigma, St. Louis, MO, USA, LYSISO1). .. After another wash, the cells were suspended in extraction buffer and subjected to Dounce homogenization (Pestle B) followed by centrifugation (1000 × g for 10 min).

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

    Activity Assay:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Article Title: Phospholipase D1 deficiency in mice causes nonalcoholic fatty liver disease via an autophagy defect
    Article Snippet: .. Isolation of lysosomal fractions and PLD activity assay Lysosomal fractions were isolated from the liver using a Lysosome Isolation Kit (LYSISO1, Sigma Aldrich) according to the manufacturer instructions. .. PLD activity was measured using Phospholipase D Assay Kit (MAK137, Sigma Aldrich) according to the manufacturer instructions.

    Cell Fractionation:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Lysosome isolation kit is from Sigma-Aldrich. .. Cell fractionation and lysosome isolation were performed according to manufacturer’s instructions.

    Modification:

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. 2.5 Isolation of Organelle From Spleen Tissues Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Acid Phosphatase Assay:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Protease Inhibitor:

    Article Title: Targetome analysis of chaperone-mediated autophagy in cancer cells
    Article Snippet: Lysosomal fractionation The lysosomal fractionation was performed according to the manufacturer of the Lysosome Isolation Kit (Sigma-Aldrich, LYSISO1). .. Cells were resuspended in 2 ml of 1x extraction buffer supplemented with Protease Inhibitor Cocktail (Sigma-Aldrich, P8340).

    Cell Culture:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: .. The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Nucleic Acid Electrophoresis:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Lysosome isolation kit is from Sigma-Aldrich. .. Protein extraction procedures, gel electrophoresis, and immunoblotting were detailed previously , .

    Isolation:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: .. The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Article Title: Phosphorylation of LAMP2A by p38 MAPK couples ER stress to chaperone-mediated autophagy
    Article Snippet: .. Isolation and subfractionation of lysosomes Lysosomes were purified from SN4741 cells using a lysosome isolation kit from Sigma (Cat# LYSISO1). ..

    Article Title: Autophagy-dependent ribosomal RNA degradation is essential for maintaining nucleotide homeostasis during C. elegans development
    Article Snippet: .. Lysosomes were purified from adult worms by a Lysosome Isolation kit (LYSISO1; Sigma-Aldrich, Missouri, USA) as described previously with modifications ( ). .. Worm pellets were suspended in the extraction buffer supplied in the Lysosome Isolation kit and ruptured by glass beads with the FastPrep−24 Instrument (MP Biomedicals, Ohio, USA).

    Article Title: Ubiquitination of Rheb governs growth factor-induced mTORC1 activation
    Article Snippet: .. Lysosomes isolation Lysosomes were isolated with lysosome Isolation Kit (Catalog Number LYSISO1, sigma), all subsequent steps of the lysosomal isolation were performed according to manufacturer’s description. .. In brief, the transfected HEK 293 T cells were harvested on ~90% confluency, and the cells were centrifuged for 5 min at 600×g .

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: .. Lysosome isolation kit is from Sigma-Aldrich. .. Cell fractionation and lysosome isolation were performed according to manufacturer’s instructions.

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. 2.5 Isolation of Organelle From Spleen Tissues Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Article Title: Oxidation of Survival Factor MEF2D in Neuronal Death and Parkinson's Disease
    Article Snippet: .. The cells and liver tissues were washed with PBS and homogenized in an extraction buffer (Lysosome Isolation Kit; Sigma). .. After separation by density gradient centrifugation, the lysosomes were isolated from the light mitochondrial–lysosomal fraction as previously described ( ).

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: .. Lysosome Isolation Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. ..

    Article Title: Autophagy-dependent ribosomal RNA degradation is essential for maintaining nucleotide homeostasis during C. elegans development
    Article Snippet: .. Lysosome purification and examination of RNA accumulation Lysosomes were purified from adult worms by a Lysosome Isolation kit (LYSISO1; Sigma-Aldrich, Missouri, USA) as described previously with modifications ( ). .. Worm pellets were suspended in the extraction buffer supplied in the Lysosome Isolation kit and ruptured by glass beads with the FastPrep−24 Instrument (MP Biomedicals, Ohio, USA).

    Article Title: Targetome analysis of chaperone-mediated autophagy in cancer cells
    Article Snippet: .. Lysosomal fractionation The lysosomal fractionation was performed according to the manufacturer of the Lysosome Isolation Kit (Sigma-Aldrich, LYSISO1). ..

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Article Title: Vitamin D3 activates the autolysosomal degradation function against Helicobacter pylori through the PDIA3 receptor in gastric epithelial cells
    Article Snippet: .. Lysosome isolation was performed according to the manufacturer’s description (Lysosome Isolation Kit; Sigma-Aldrich, LYSISO1). ..

    Article Title: Eucommia ulmoides Leaf Extract Ameliorates Steatosis Induced by High-fat Diet in Rats by Increasing Lysosomal Function
    Article Snippet: .. Lysosomal Fractionation Lysosomal fractionation was conducted using the Lysosome Isolation Kit (Sigma, St. Louis, MO, USA, LYSISO1). ..

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: .. Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. ..

    Transfection:

    Article Title: Ubiquitination of Rheb governs growth factor-induced mTORC1 activation
    Article Snippet: Lysosomes isolation Lysosomes were isolated with lysosome Isolation Kit (Catalog Number LYSISO1, sigma), all subsequent steps of the lysosomal isolation were performed according to manufacturer’s description. .. In brief, the transfected HEK 293 T cells were harvested on ~90% confluency, and the cells were centrifuged for 5 min at 600×g .

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Transfected cells were re-plated in LabTek chamber coverglasses (NUNC) grown overnight at 37°C with 5% CO2 in a PeCon open chamber (PeCon, Erbach, Germany) for microscopy. .. Lysosome isolation kit is from Sigma-Aldrich.

    Labeling:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Ultrathin cryosectioning and immunogold labeling were previously described . .. Lysosome isolation kit is from Sigma-Aldrich.

    Mouse Assay:

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. 2.5 Isolation of Organelle From Spleen Tissues Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: .. Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). ..

    Protein Extraction:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Lysosome isolation kit is from Sigma-Aldrich. .. Protein extraction procedures, gel electrophoresis, and immunoblotting were detailed previously , .

    Microscopy:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Transfected cells were re-plated in LabTek chamber coverglasses (NUNC) grown overnight at 37°C with 5% CO2 in a PeCon open chamber (PeCon, Erbach, Germany) for microscopy. .. Lysosome isolation kit is from Sigma-Aldrich.

    Purification:

    Article Title: Phosphorylation of LAMP2A by p38 MAPK couples ER stress to chaperone-mediated autophagy
    Article Snippet: .. Isolation and subfractionation of lysosomes Lysosomes were purified from SN4741 cells using a lysosome isolation kit from Sigma (Cat# LYSISO1). ..

    Article Title: Autophagy-dependent ribosomal RNA degradation is essential for maintaining nucleotide homeostasis during C. elegans development
    Article Snippet: .. Lysosomes were purified from adult worms by a Lysosome Isolation kit (LYSISO1; Sigma-Aldrich, Missouri, USA) as described previously with modifications ( ). .. Worm pellets were suspended in the extraction buffer supplied in the Lysosome Isolation kit and ruptured by glass beads with the FastPrep−24 Instrument (MP Biomedicals, Ohio, USA).

    Article Title: Autophagy-dependent ribosomal RNA degradation is essential for maintaining nucleotide homeostasis during C. elegans development
    Article Snippet: .. Lysosome purification and examination of RNA accumulation Lysosomes were purified from adult worms by a Lysosome Isolation kit (LYSISO1; Sigma-Aldrich, Missouri, USA) as described previously with modifications ( ). .. Worm pellets were suspended in the extraction buffer supplied in the Lysosome Isolation kit and ruptured by glass beads with the FastPrep−24 Instrument (MP Biomedicals, Ohio, USA).

    Software:

    Article Title: Autophagy termination and lysosome reformation regulated by mTOR
    Article Snippet: Images were digitally acquired by a Leica TCS SP5 confocal microsystem and analyzed using instrument software and by the IMARIS package (Bitplane, Switzerland). .. Lysosome isolation kit is from Sigma-Aldrich.

    Homogenization:

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: 2.5 Isolation of Organelle From Spleen Tissues Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). .. Another 1 mL Extraction buffer was added to the pellet and the homogenization was repeated.

    Article Title: Chitinase-3-like Protein 1: A Progranulin Downstream Molecule and Potential Biomarker for Gaucher Disease
    Article Snippet: Spleens from WT and PGRN KO mice with OVA challenge were dissected and the cellular organelles were isolated by using a modified protocol of the Lysosome Isolation Kit (Sigma-Aldrich). .. Another 1 mL Extraction buffer was added to the pellet and the homogenization was repeated.

    Article Title: Eucommia ulmoides Leaf Extract Ameliorates Steatosis Induced by High-fat Diet in Rats by Increasing Lysosomal Function
    Article Snippet: Lysosomal Fractionation Lysosomal fractionation was conducted using the Lysosome Isolation Kit (Sigma, St. Louis, MO, USA, LYSISO1). .. After another wash, the cells were suspended in extraction buffer and subjected to Dounce homogenization (Pestle B) followed by centrifugation (1000 × g for 10 min).

    Spectrophotometry:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The acid phosphatase activity was presented by the conversion of 4-nitrophenyl phosphate to 4-nitrophenol that was measured by spectrophotometer at 405 nm ( , ).

    Concentration Assay:

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosome Isolation Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

    Fractionation:

    Article Title: Targetome analysis of chaperone-mediated autophagy in cancer cells
    Article Snippet: .. Lysosomal fractionation The lysosomal fractionation was performed according to the manufacturer of the Lysosome Isolation Kit (Sigma-Aldrich, LYSISO1). ..

    Article Title: Eucommia ulmoides Leaf Extract Ameliorates Steatosis Induced by High-fat Diet in Rats by Increasing Lysosomal Function
    Article Snippet: .. Lysosomal Fractionation Lysosomal fractionation was conducted using the Lysosome Isolation Kit (Sigma, St. Louis, MO, USA, LYSISO1). ..

    Marker:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Gradient Centrifugation:

    Article Title: Reconstitution of lysosomal NAADP-TRP-ML1 signaling pathway and its function in TRP-ML1−/− cells
    Article Snippet: .. The lysosomes from cultured human fibroblasts were isolated by a lysosome isolation kit (LYSISO1, Sigma) with differential centrifugation, followed by a density gradient centrifugation and Ca2+ precipitation, as detailed in the manufacturer's manual. .. The purity of the prepared lysosomes from fibroblasts was biochemically determined by measuring the activity of acid phosphatase, a lysosomal marker enzyme, with the acid phosphatase assay kit (CS0740, Sigma).

    Article Title: Oxidation of Survival Factor MEF2D in Neuronal Death and Parkinson's Disease
    Article Snippet: The cells and liver tissues were washed with PBS and homogenized in an extraction buffer (Lysosome Isolation Kit; Sigma). .. After separation by density gradient centrifugation, the lysosomes were isolated from the light mitochondrial–lysosomal fraction as previously described ( ).

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosome Isolation Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

    Article Title: Ac2-26 Induces IKKβ Degradation Through Chaperone-Mediated Autophagy Via HSPB1 in NCM-Treated Microglia
    Article Snippet: Lysosomes were isolated from brain tissue using a Lysosome Isolation kit (Sigma) according to the kit protocol. .. The mixed medium was separated by density gradient centrifugation (150,000× g for 4 h) with Beckman Coulter Optimal L-80 XP Ultracentrifue and SW 55Ti Swing out Rotor in a multistep OptiPrep gradient, and calcium chloride was added to a final concentration of 8 mM for low-speed (5000× g for 15 min) centrifugation.

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    Lysosomes are organelles ubiquitous in most prokaryotic and eukaryotic cells They contain many acid hydrolases that take part in protein degradation in the cell Lysosomes also contain lipases nucleases and
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