lysis buffer b  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    pH 4 Buffer
    Description:
    This Certified Reference Material CRM is produced and certified in accordance with ISO 17034 and ISO IEC 17025 All information regarding the use of this CRM can be found on the certificate of analysis
    Catalog Number:
    qc1121
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore lysis buffer b
    This Certified Reference Material CRM is produced and certified in accordance with ISO 17034 and ISO IEC 17025 All information regarding the use of this CRM can be found on the certificate of analysis
    https://www.bioz.com/result/lysis buffer b/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    lysis buffer b - by Bioz Stars, 2020-07
    99/100 stars

    Images

    Related Articles

    Co-Immunoprecipitation Assay:

    Article Title: A Positive Role of c-Myc in Regulating Androgen Receptor and its Splice Variants in Prostate Cancer
    Article Snippet: .. Co-immunoprecipitation assay Cells were pelleted, washed twice with cold PBS, and lysed in 500 μl of lysis buffer (1% NP-40, 20 mmol/L Tris, pH 7.4, 140 mmol/L NaCl, and 2 mmol/L EDTA) containing a protease inhibitor cocktail (Sigma). .. The lysate was then used for immunoprecipitation with a c-Myc antibody (Sigma) or an IgG control.

    Sonication:

    Article Title: Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
    Article Snippet: .. The cells were suspended in 6 ml lysis buffer [50 m M NaH2 PO4 pH 8.0, 500 m M NaCl, 10 m M MgCl2 , 3 m M β-mercaptoethanol (β-ME), 1 m M 4-(2-aminoethyl)benzenesulfonylfluoride, 0.1%( v / v ) Triton X-­100, 0.1 µg ml−1 lysozyme and 12.5 U ml−1 Benzonase nuclease (Novagen)] per gram wet weight and incubated at 294 K for 30 min. Lysis was performed by sonication for 5 min in 15 s pulses at 277 K. Cell debris was removed by centrifugation at 15 500 g for 20 min at 277 K. 1 g Protino Ni–IDA matrix (Macherey-Nagel) was added per 50 ml crude lysate and the slurry was incubated for 1–2 h at 277 K in order to allow complete binding. ..

    Protease Inhibitor:

    Article Title: A Positive Role of c-Myc in Regulating Androgen Receptor and its Splice Variants in Prostate Cancer
    Article Snippet: .. Co-immunoprecipitation assay Cells were pelleted, washed twice with cold PBS, and lysed in 500 μl of lysis buffer (1% NP-40, 20 mmol/L Tris, pH 7.4, 140 mmol/L NaCl, and 2 mmol/L EDTA) containing a protease inhibitor cocktail (Sigma). .. The lysate was then used for immunoprecipitation with a c-Myc antibody (Sigma) or an IgG control.

    Article Title: Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE 1
    Article Snippet: .. For SbSnRK1 phosphorylation, frozen samples were homogenized and incubated in lysis buffer (137 m m NaCl, 2.7 m m KCl, 10 m m Na2 HPO4 , 2 m m KH2 PO4 , pH 7.5, 1 m m EDTA, 0.05% [v/v] Triton X-100, 1:500 protease inhibitor cocktail [Sigma; P9599], and 1% [v/v] phosphatase inhibitor cocktail 3 [Sigma; P0044]) for 5 min. After centrifugation at 18,000 g at 4°C for 15 min, the protein concentration was quantified via the manufacturer’s protocol. .. Equal amounts of lysate (300 μg) were separated on a 10% (w/v) SDS-PAGE gel and then blotted onto a polyvinylidene difluoride membrane (0.45 μm; Millipore) that was analyzed with the phospho-AMPKα (Thr-172) polyclonal antibody (Cell Signaling Technology), which recognizes the highly conserved sequence of phosphorylated SbSnRK1 or PKIN1.

    Article Title: Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice
    Article Snippet: .. The brains were collected from neonatal mice at 24 h after the final polyI:C treatment on PD7 and homogenized with lysis buffer (40 mM Tris‐HCl [pH 7.4], 2 mM EDTA, 100 mM NaCl, and 0.5% Triton X‐100, and protease inhibitor cocktail [Sigma‐Aldrich]). .. Lysate protein (1000 mg) collected from COS7 cells and neonatal mouse brain was added to Protein A/G Plus Agarose cross‐linked to rabbit anti‐HA (Sigma‐Aldrich), rabbit anti‐ATP6V0B (Abgent, San Diego, CA), mouse anti‐kinesin heavy chain (Millipore), rabbit anti‐visfatin (Bethyl Laboratories, Montgomery, TX), normal rabbit IgG, or normal mouse IgG (Santa Cruz Biotechnology), and incubated overnight at 4°C.

    Article Title: Maternal insulin resistance multigenerationally impairs synaptic plasticity and memory via gametic mechanisms
    Article Snippet: .. Western blotting Tissues (hippocampi or ovaries) were lysed in ice-cold lysis buffer (NaCl 150 mM, Tris-HCl 50 mM pH 7.4, EDTA 2 mM) containing 1% Triton X-100, 0.1% SDS, 1 × protease inhibitor cocktail (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), and 1 mM sodium fluoride (Sigma-Aldrich). .. Cells were incubated for 10 min on ice with occasional vortexing and spun down at 22,000 × g , 4 °C.

    Centrifugation:

    Article Title: Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
    Article Snippet: .. The cells were suspended in 6 ml lysis buffer [50 m M NaH2 PO4 pH 8.0, 500 m M NaCl, 10 m M MgCl2 , 3 m M β-mercaptoethanol (β-ME), 1 m M 4-(2-aminoethyl)benzenesulfonylfluoride, 0.1%( v / v ) Triton X-­100, 0.1 µg ml−1 lysozyme and 12.5 U ml−1 Benzonase nuclease (Novagen)] per gram wet weight and incubated at 294 K for 30 min. Lysis was performed by sonication for 5 min in 15 s pulses at 277 K. Cell debris was removed by centrifugation at 15 500 g for 20 min at 277 K. 1 g Protino Ni–IDA matrix (Macherey-Nagel) was added per 50 ml crude lysate and the slurry was incubated for 1–2 h at 277 K in order to allow complete binding. ..

    Article Title: Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE 1
    Article Snippet: .. For SbSnRK1 phosphorylation, frozen samples were homogenized and incubated in lysis buffer (137 m m NaCl, 2.7 m m KCl, 10 m m Na2 HPO4 , 2 m m KH2 PO4 , pH 7.5, 1 m m EDTA, 0.05% [v/v] Triton X-100, 1:500 protease inhibitor cocktail [Sigma; P9599], and 1% [v/v] phosphatase inhibitor cocktail 3 [Sigma; P0044]) for 5 min. After centrifugation at 18,000 g at 4°C for 15 min, the protein concentration was quantified via the manufacturer’s protocol. .. Equal amounts of lysate (300 μg) were separated on a 10% (w/v) SDS-PAGE gel and then blotted onto a polyvinylidene difluoride membrane (0.45 μm; Millipore) that was analyzed with the phospho-AMPKα (Thr-172) polyclonal antibody (Cell Signaling Technology), which recognizes the highly conserved sequence of phosphorylated SbSnRK1 or PKIN1.

    Incubation:

    Article Title: Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
    Article Snippet: .. The cells were suspended in 6 ml lysis buffer [50 m M NaH2 PO4 pH 8.0, 500 m M NaCl, 10 m M MgCl2 , 3 m M β-mercaptoethanol (β-ME), 1 m M 4-(2-aminoethyl)benzenesulfonylfluoride, 0.1%( v / v ) Triton X-­100, 0.1 µg ml−1 lysozyme and 12.5 U ml−1 Benzonase nuclease (Novagen)] per gram wet weight and incubated at 294 K for 30 min. Lysis was performed by sonication for 5 min in 15 s pulses at 277 K. Cell debris was removed by centrifugation at 15 500 g for 20 min at 277 K. 1 g Protino Ni–IDA matrix (Macherey-Nagel) was added per 50 ml crude lysate and the slurry was incubated for 1–2 h at 277 K in order to allow complete binding. ..

    Article Title: Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE 1
    Article Snippet: .. For SbSnRK1 phosphorylation, frozen samples were homogenized and incubated in lysis buffer (137 m m NaCl, 2.7 m m KCl, 10 m m Na2 HPO4 , 2 m m KH2 PO4 , pH 7.5, 1 m m EDTA, 0.05% [v/v] Triton X-100, 1:500 protease inhibitor cocktail [Sigma; P9599], and 1% [v/v] phosphatase inhibitor cocktail 3 [Sigma; P0044]) for 5 min. After centrifugation at 18,000 g at 4°C for 15 min, the protein concentration was quantified via the manufacturer’s protocol. .. Equal amounts of lysate (300 μg) were separated on a 10% (w/v) SDS-PAGE gel and then blotted onto a polyvinylidene difluoride membrane (0.45 μm; Millipore) that was analyzed with the phospho-AMPKα (Thr-172) polyclonal antibody (Cell Signaling Technology), which recognizes the highly conserved sequence of phosphorylated SbSnRK1 or PKIN1.

    Mouse Assay:

    Article Title: Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice
    Article Snippet: .. The brains were collected from neonatal mice at 24 h after the final polyI:C treatment on PD7 and homogenized with lysis buffer (40 mM Tris‐HCl [pH 7.4], 2 mM EDTA, 100 mM NaCl, and 0.5% Triton X‐100, and protease inhibitor cocktail [Sigma‐Aldrich]). .. Lysate protein (1000 mg) collected from COS7 cells and neonatal mouse brain was added to Protein A/G Plus Agarose cross‐linked to rabbit anti‐HA (Sigma‐Aldrich), rabbit anti‐ATP6V0B (Abgent, San Diego, CA), mouse anti‐kinesin heavy chain (Millipore), rabbit anti‐visfatin (Bethyl Laboratories, Montgomery, TX), normal rabbit IgG, or normal mouse IgG (Santa Cruz Biotechnology), and incubated overnight at 4°C.

    Protein Extraction:

    Article Title: Biochemical analysis of antimicrobial peptides in two different Capsicum genotypes after fruit infection by Colletotrichum gloeosporioides
    Article Snippet: .. About 40 g of inoculated fruits (without peduncle and seeds) and 40 g of non-inoculated fruits (without peduncle and seeds) (controls) from each interval were used for protein extraction The fruit powders were processed in 200 ml of extraction buffer (10 mM Na2 HPO4 , 15 mM NaH2 PO4 , 100 mM KCl, 1.5% EDTA, pH 5.4) (Sigma) for 15 min with a multiprocessor, and the mixture was shaked for 2 h at 4°C. ..

    Protein Concentration:

    Article Title: Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE 1
    Article Snippet: .. For SbSnRK1 phosphorylation, frozen samples were homogenized and incubated in lysis buffer (137 m m NaCl, 2.7 m m KCl, 10 m m Na2 HPO4 , 2 m m KH2 PO4 , pH 7.5, 1 m m EDTA, 0.05% [v/v] Triton X-100, 1:500 protease inhibitor cocktail [Sigma; P9599], and 1% [v/v] phosphatase inhibitor cocktail 3 [Sigma; P0044]) for 5 min. After centrifugation at 18,000 g at 4°C for 15 min, the protein concentration was quantified via the manufacturer’s protocol. .. Equal amounts of lysate (300 μg) were separated on a 10% (w/v) SDS-PAGE gel and then blotted onto a polyvinylidene difluoride membrane (0.45 μm; Millipore) that was analyzed with the phospho-AMPKα (Thr-172) polyclonal antibody (Cell Signaling Technology), which recognizes the highly conserved sequence of phosphorylated SbSnRK1 or PKIN1.

    Western Blot:

    Article Title: Maternal insulin resistance multigenerationally impairs synaptic plasticity and memory via gametic mechanisms
    Article Snippet: .. Western blotting Tissues (hippocampi or ovaries) were lysed in ice-cold lysis buffer (NaCl 150 mM, Tris-HCl 50 mM pH 7.4, EDTA 2 mM) containing 1% Triton X-100, 0.1% SDS, 1 × protease inhibitor cocktail (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), and 1 mM sodium fluoride (Sigma-Aldrich). .. Cells were incubated for 10 min on ice with occasional vortexing and spun down at 22,000 × g , 4 °C.

    Lysis:

    Article Title: A Positive Role of c-Myc in Regulating Androgen Receptor and its Splice Variants in Prostate Cancer
    Article Snippet: .. Co-immunoprecipitation assay Cells were pelleted, washed twice with cold PBS, and lysed in 500 μl of lysis buffer (1% NP-40, 20 mmol/L Tris, pH 7.4, 140 mmol/L NaCl, and 2 mmol/L EDTA) containing a protease inhibitor cocktail (Sigma). .. The lysate was then used for immunoprecipitation with a c-Myc antibody (Sigma) or an IgG control.

    Article Title: Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
    Article Snippet: .. The cells were suspended in 6 ml lysis buffer [50 m M NaH2 PO4 pH 8.0, 500 m M NaCl, 10 m M MgCl2 , 3 m M β-mercaptoethanol (β-ME), 1 m M 4-(2-aminoethyl)benzenesulfonylfluoride, 0.1%( v / v ) Triton X-­100, 0.1 µg ml−1 lysozyme and 12.5 U ml−1 Benzonase nuclease (Novagen)] per gram wet weight and incubated at 294 K for 30 min. Lysis was performed by sonication for 5 min in 15 s pulses at 277 K. Cell debris was removed by centrifugation at 15 500 g for 20 min at 277 K. 1 g Protino Ni–IDA matrix (Macherey-Nagel) was added per 50 ml crude lysate and the slurry was incubated for 1–2 h at 277 K in order to allow complete binding. ..

    Article Title: Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE 1
    Article Snippet: .. For SbSnRK1 phosphorylation, frozen samples were homogenized and incubated in lysis buffer (137 m m NaCl, 2.7 m m KCl, 10 m m Na2 HPO4 , 2 m m KH2 PO4 , pH 7.5, 1 m m EDTA, 0.05% [v/v] Triton X-100, 1:500 protease inhibitor cocktail [Sigma; P9599], and 1% [v/v] phosphatase inhibitor cocktail 3 [Sigma; P0044]) for 5 min. After centrifugation at 18,000 g at 4°C for 15 min, the protein concentration was quantified via the manufacturer’s protocol. .. Equal amounts of lysate (300 μg) were separated on a 10% (w/v) SDS-PAGE gel and then blotted onto a polyvinylidene difluoride membrane (0.45 μm; Millipore) that was analyzed with the phospho-AMPKα (Thr-172) polyclonal antibody (Cell Signaling Technology), which recognizes the highly conserved sequence of phosphorylated SbSnRK1 or PKIN1.

    Article Title: Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice
    Article Snippet: .. The brains were collected from neonatal mice at 24 h after the final polyI:C treatment on PD7 and homogenized with lysis buffer (40 mM Tris‐HCl [pH 7.4], 2 mM EDTA, 100 mM NaCl, and 0.5% Triton X‐100, and protease inhibitor cocktail [Sigma‐Aldrich]). .. Lysate protein (1000 mg) collected from COS7 cells and neonatal mouse brain was added to Protein A/G Plus Agarose cross‐linked to rabbit anti‐HA (Sigma‐Aldrich), rabbit anti‐ATP6V0B (Abgent, San Diego, CA), mouse anti‐kinesin heavy chain (Millipore), rabbit anti‐visfatin (Bethyl Laboratories, Montgomery, TX), normal rabbit IgG, or normal mouse IgG (Santa Cruz Biotechnology), and incubated overnight at 4°C.

    Article Title: Melatonin receptor signaling contributes to neuroprotection upon arousal from torpor in thirteen-lined ground squirrels
    Article Snippet: .. Samples were homogenized in lysis buffer (50 mM HEPES, pH 7.4, 5 mM CHAPS, and 5 mM DTT) with aprotonin (6.5 μg/ml; A1153; Sigma-Aldrich). .. The supernatant was collected and protein concentration was determined using a bicinchoninic acid assay (BCA; no. 23255; Thermo Fisher Scientific) according to the manufacturer's instructions.

    Article Title: Maternal insulin resistance multigenerationally impairs synaptic plasticity and memory via gametic mechanisms
    Article Snippet: .. Western blotting Tissues (hippocampi or ovaries) were lysed in ice-cold lysis buffer (NaCl 150 mM, Tris-HCl 50 mM pH 7.4, EDTA 2 mM) containing 1% Triton X-100, 0.1% SDS, 1 × protease inhibitor cocktail (Sigma-Aldrich), 1 mM sodium orthovanadate (Sigma-Aldrich), and 1 mM sodium fluoride (Sigma-Aldrich). .. Cells were incubated for 10 min on ice with occasional vortexing and spun down at 22,000 × g , 4 °C.

    Binding Assay:

    Article Title: Cloning, expression, purification and crystallization as well as X-ray fluorescence and preliminary X-ray diffraction analyses of human ADP-ribosylhydrolase 1
    Article Snippet: .. The cells were suspended in 6 ml lysis buffer [50 m M NaH2 PO4 pH 8.0, 500 m M NaCl, 10 m M MgCl2 , 3 m M β-mercaptoethanol (β-ME), 1 m M 4-(2-aminoethyl)benzenesulfonylfluoride, 0.1%( v / v ) Triton X-­100, 0.1 µg ml−1 lysozyme and 12.5 U ml−1 Benzonase nuclease (Novagen)] per gram wet weight and incubated at 294 K for 30 min. Lysis was performed by sonication for 5 min in 15 s pulses at 277 K. Cell debris was removed by centrifugation at 15 500 g for 20 min at 277 K. 1 g Protino Ni–IDA matrix (Macherey-Nagel) was added per 50 ml crude lysate and the slurry was incubated for 1–2 h at 277 K in order to allow complete binding. ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore lysis buffer l b
    Lysis Buffer L B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer l b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysis buffer l b - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore lysis buffer b
    Lysis Buffer B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer b/product/Millipore
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    lysis buffer b - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    85
    Millipore np 40 lysis buffer b
    Np 40 Lysis Buffer B, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/np 40 lysis buffer b/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    np 40 lysis buffer b - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results