blastocystis antigen  (Roche)


Bioz Verified Symbol Roche is a verified supplier
Bioz Manufacturer Symbol Roche manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88

    Structured Review

    Roche blastocystis antigen
    Serum antibodies from <t>Blastocystis</t> –positive clinical subgroups IBS-P and HC-P, and Blastofluor® Ab reacting with Blastocystis proteins in Western blot
    Blastocystis Antigen, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 33524 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blastocystis antigen/product/Roche
    Average 88 stars, based on 33524 article reviews
    Price from $9.99 to $1999.99
    blastocystis antigen - by Bioz Stars, 2020-09
    88/100 stars

    Images

    1) Product Images from "Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls"

    Article Title: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls

    Journal: Parasites & Vectors

    doi: 10.1186/s13071-015-1069-x

    Serum antibodies from Blastocystis –positive clinical subgroups IBS-P and HC-P, and Blastofluor® Ab reacting with Blastocystis proteins in Western blot
    Figure Legend Snippet: Serum antibodies from Blastocystis –positive clinical subgroups IBS-P and HC-P, and Blastofluor® Ab reacting with Blastocystis proteins in Western blot

    Techniques Used: Western Blot

    Serum antibodies from clinical subgroup IBS-P and monoclonal antibodies MAb1D5 and MAb5 reacting with Blastocystis proteins in Western blot
    Figure Legend Snippet: Serum antibodies from clinical subgroup IBS-P and monoclonal antibodies MAb1D5 and MAb5 reacting with Blastocystis proteins in Western blot

    Techniques Used: Western Blot

    Serum antibodies from Blastocystis negative clinical subgroups IBS-N and HC-N reacting with Blastocystis proteins in Western blot
    Figure Legend Snippet: Serum antibodies from Blastocystis negative clinical subgroups IBS-N and HC-N reacting with Blastocystis proteins in Western blot

    Techniques Used: Western Blot

    Serum antibodies from Blastocystis positive clinical subgroups IBS-P and HC-P reacting with Blastocystis proteins in Western blot
    Figure Legend Snippet: Serum antibodies from Blastocystis positive clinical subgroups IBS-P and HC-P reacting with Blastocystis proteins in Western blot

    Techniques Used: Western Blot

    Serum antibodies in IBS-P clinical subgroup and MAb anti-proMMP-9 reacting with Blastocystis proteins in Western blot
    Figure Legend Snippet: Serum antibodies in IBS-P clinical subgroup and MAb anti-proMMP-9 reacting with Blastocystis proteins in Western blot

    Techniques Used: Western Blot

    Related Articles

    Centrifugation:

    Article Title: A l-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily
    Article Snippet: .. Subsequently, the culture was induced with 0.02% l- arabinose and incubated at 37 °C for 3 h. When the suspension reached an A 600/cm of 2, the cells were harvested by centrifugation and resuspended in 70 μl of lysis buffer consisting of 20 m m HEPES-NaOH, 100 m m NaCl, 10 m m MgSO4 pH 7.5, 1× Complete protease inhibitor mixture (Roche Applied Science), 1 mg/ml lysozyme (Applichem, Darmstadt), 1 unit of Benzonase (Merck KGaA). .. After 20 min of incubation at room temperature, 5 μl of 10% SDS and 25 μl of 4× SDS-PAGE loading buffer were added to the mixture.

    Article Title: The N0-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient ?-helices
    Article Snippet: .. After an incubation of 3 h, cells were harvested by centrifugation and resuspended in 20 m M Tris-HCl, 150 m M NaCl, 50 m M Glu, and 50 m M Arg, pH 7.5 (Buffer A) supplemented with antiproteases (Complete™ Protease Inhibitor Cocktail Tablets, Roche), and cells were disrupted by sonication. ..

    Protease Inhibitor:

    Article Title: TPD52 expression increases neutral lipid storage within cultured cells
    Article Snippet: .. Briefly, D52-2-7 cells were treated with 400 µM oleic acid in fatty-acid-free BSA for 24 h and then lysed in hypotonic lysis medium [HLM; 40 mM Tris–HCl pH 7.4, 2 mM EDTA, 20 mM sodium fluoride, and EDTA-free protease inhibitor cocktail tablets (Roche Applied Science, NSW, Australia)). .. After incubation on ice for 10 min, cell lysates were briefly sonicated and subjected to centrifugation at 1000 g for 10 min.

    Article Title: Identification of a Novel Zn2+-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin *-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin * S⃞
    Article Snippet: .. The E. coli cells were harvested and lysed by sonication on ice in 20 m m Tris-HCl (pH 7.4), 120 m m NaCl, 1 m m dithiothreitol buffer supplemented with EDTA-free protease inhibitor mixture (Roche Applied Science). .. Triton X-100 was added to the cell lysate to a final concentration of 1%, and the sample was incubated at 4 °C for 30 min with rotation.

    Article Title: A l-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily
    Article Snippet: .. Subsequently, the culture was induced with 0.02% l- arabinose and incubated at 37 °C for 3 h. When the suspension reached an A 600/cm of 2, the cells were harvested by centrifugation and resuspended in 70 μl of lysis buffer consisting of 20 m m HEPES-NaOH, 100 m m NaCl, 10 m m MgSO4 pH 7.5, 1× Complete protease inhibitor mixture (Roche Applied Science), 1 mg/ml lysozyme (Applichem, Darmstadt), 1 unit of Benzonase (Merck KGaA). .. After 20 min of incubation at room temperature, 5 μl of 10% SDS and 25 μl of 4× SDS-PAGE loading buffer were added to the mixture.

    Article Title: The N0-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient ?-helices
    Article Snippet: .. After an incubation of 3 h, cells were harvested by centrifugation and resuspended in 20 m M Tris-HCl, 150 m M NaCl, 50 m M Glu, and 50 m M Arg, pH 7.5 (Buffer A) supplemented with antiproteases (Complete™ Protease Inhibitor Cocktail Tablets, Roche), and cells were disrupted by sonication. ..

    Incubation:

    Article Title: A l-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily
    Article Snippet: .. Subsequently, the culture was induced with 0.02% l- arabinose and incubated at 37 °C for 3 h. When the suspension reached an A 600/cm of 2, the cells were harvested by centrifugation and resuspended in 70 μl of lysis buffer consisting of 20 m m HEPES-NaOH, 100 m m NaCl, 10 m m MgSO4 pH 7.5, 1× Complete protease inhibitor mixture (Roche Applied Science), 1 mg/ml lysozyme (Applichem, Darmstadt), 1 unit of Benzonase (Merck KGaA). .. After 20 min of incubation at room temperature, 5 μl of 10% SDS and 25 μl of 4× SDS-PAGE loading buffer were added to the mixture.

    Article Title: The N0-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient ?-helices
    Article Snippet: .. After an incubation of 3 h, cells were harvested by centrifugation and resuspended in 20 m M Tris-HCl, 150 m M NaCl, 50 m M Glu, and 50 m M Arg, pH 7.5 (Buffer A) supplemented with antiproteases (Complete™ Protease Inhibitor Cocktail Tablets, Roche), and cells were disrupted by sonication. ..

    Inhibition:

    Article Title: First Identification of Resident and Circulating Fibrocytes in Dupuytren's Disease Shown to Be Inhibited by Serum Amyloid P and Xiapex
    Article Snippet: .. Xiapex is More Effective in Fibrocyte Inhibition in DD Compared to Collagenase A We set out to determine whether Xiapex (a special formulation of Collagenase Clostridium histolyticum by Auxilium Pharmaceuticals, USA) is more effective in inhibiting fibrocyte differentiation compared to commercially available collagenase A (Roche Diagnostics, UK). ..

    Sonication:

    Article Title: Identification of a Novel Zn2+-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin *-binding Domain in the Autosomal Recessive Juvenile Parkinson-related E3 Ligase Parkin * S⃞
    Article Snippet: .. The E. coli cells were harvested and lysed by sonication on ice in 20 m m Tris-HCl (pH 7.4), 120 m m NaCl, 1 m m dithiothreitol buffer supplemented with EDTA-free protease inhibitor mixture (Roche Applied Science). .. Triton X-100 was added to the cell lysate to a final concentration of 1%, and the sample was incubated at 4 °C for 30 min with rotation.

    Article Title: The N0-binding region of the vesicular stomatitis virus phosphoprotein is globally disordered but contains transient ?-helices
    Article Snippet: .. After an incubation of 3 h, cells were harvested by centrifugation and resuspended in 20 m M Tris-HCl, 150 m M NaCl, 50 m M Glu, and 50 m M Arg, pH 7.5 (Buffer A) supplemented with antiproteases (Complete™ Protease Inhibitor Cocktail Tablets, Roche), and cells were disrupted by sonication. ..

    FACS:

    Article Title: High-dimensional analysis of intestinal immune cells during helminth infection
    Article Snippet: .. Reagents RPMI (Gibco RPMI Medium 1640 #11875–093) HBSS (Gibco Hank’s Balanced Salt Solution #14175–095) DPBS (Gibco Dulbecco’s Phosphate Buffered Saline #14190–144) UltraPure 0.5M EDTA (Thermofisher #15575020) DNAse I (Roche #10104159001, 2916 Kunitz units/mgL) Collagenase A from Clostridium histolyticum (Roche #10103578001, 0.223 U/mg solid) Fetal Bovine Serum (Gibco) FACS buffer (PBS, 2% FBS, 2 mM EDTA) EDTA wash buffer (HBSS, 2 mM EDTA) Wash buffer (HBSS) Collection buffer (HBSS, 2% FBS) Digestion mix (RPMI, 20% FBS, 1 mg/ml Collagenase A, 0.05 mg/ml DNAse) Trypan Blue Solution (Gibco) .. Materials Funnels 10 × 10 cm Gauze (140 µm mesh size) Tweezers to hold gauze in place 50 ml falcon tubes 40 µm cell strainers 100 µm cell strainer 25 ml serological pipette Scissor/tweezers for collection Petri dish Haemocytometer

    Lysis:

    Article Title: Blastocystis specific serum immunoglobulin in patients with irritable bowel syndrome (IBS) versus healthy controls
    Article Snippet: .. Blastocystis antigen was prepared using the proteinase inhibitor Complete Lysis-B (2x), EDTA-free kit (Roche, Mannheim, Germany:04719948001) and stored at −20 °C until required. .. SDS-PAGE protocol Approximately 300 ng of Blastocystis antigen was mixed with equal volumes of loading buffer (Laemmli sample buffer mixed with 2 M 2-mercaptoethanol in a ratio of 19:1vol:vol (Bio- Rad, California, USA)) and loaded into the large well of a preparative 4-15 % Tris–HCl Ready Gel (Bio-Rad: 161–1140).

    Article Title: Degradation of integral membrane proteins modified with the photosensitive degron module requires the cytosolic endoplasmic reticulum–associated degradation pathway
    Article Snippet: .. Briefly, cells in exponential growth phase (culture size 50 ml) were harvested, washed with 4 ml of cold H2 O containing 1 mM phenylmethylsulfonyl fluoride (PMSF), 10 mM NaN3 , and 20 mM N-ethyl maleimide (NEM) and lysed with glass beads in lysis buffer (6 M urea, 50 mM Tris/HCl, pH 7.5, 150 mM NaCl, 1% SDS, 1 mM PMSF, 20 mM NEM, and Roche complete inhibitor mix). .. Cell debris was removed by centrifugation and the lysate was diluted with nine volumes of IP dilution buffer (55 mM Tris/HCl, pH 7.5, 165 mM NaCl, 5.5 mM EDTA, 1.1% Triton X-100, 1 mM PMSF, 20 mM NEM, and Roche complete inhibitor mix).

    Article Title: TPD52 expression increases neutral lipid storage within cultured cells
    Article Snippet: .. Briefly, D52-2-7 cells were treated with 400 µM oleic acid in fatty-acid-free BSA for 24 h and then lysed in hypotonic lysis medium [HLM; 40 mM Tris–HCl pH 7.4, 2 mM EDTA, 20 mM sodium fluoride, and EDTA-free protease inhibitor cocktail tablets (Roche Applied Science, NSW, Australia)). .. After incubation on ice for 10 min, cell lysates were briefly sonicated and subjected to centrifugation at 1000 g for 10 min.

    Article Title: A l-Lysine Transporter of High Stereoselectivity of the Amino Acid-Polyamine-Organocation (APC) Superfamily
    Article Snippet: .. Subsequently, the culture was induced with 0.02% l- arabinose and incubated at 37 °C for 3 h. When the suspension reached an A 600/cm of 2, the cells were harvested by centrifugation and resuspended in 70 μl of lysis buffer consisting of 20 m m HEPES-NaOH, 100 m m NaCl, 10 m m MgSO4 pH 7.5, 1× Complete protease inhibitor mixture (Roche Applied Science), 1 mg/ml lysozyme (Applichem, Darmstadt), 1 unit of Benzonase (Merck KGaA). .. After 20 min of incubation at room temperature, 5 μl of 10% SDS and 25 μl of 4× SDS-PAGE loading buffer were added to the mixture.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    Roche lysis b edta free kit
    Lysis B Edta Free Kit, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis b edta free kit/product/Roche
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysis b edta free kit - by Bioz Stars, 2020-09
    90/100 stars
      Buy from Supplier

    Image Search Results