lysis b buffer  (Millipore)


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  • 99
    Name:
    Leupeptin
    Description:

    Catalog Number:
    l8511
    Price:
    None
    Buy from Supplier


    Structured Review

    Millipore lysis b buffer
    Leupeptin

    https://www.bioz.com/result/lysis b buffer/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysis b buffer - by Bioz Stars, 2020-05
    99/100 stars

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    Related Articles

    Gas Chromatography:

    Article Title: Mannose 6-Phosphate/Insulin-like Growth Factor-II Receptor Targets the Urokinase Receptor to Lysosomes via a Novel Binding Interaction
    Article Snippet: .. Reagents Glucose 6-phosphate (Glc-6-P), mannose 6-phosphate, and N -propyl-galleat, leupeptin, and pepstatin A were from Sigma Chemical Co. (St. Louis, MO), and Na125 I, d -[2-3 H]mannose, and Pro-mix (l -[35 S]methionine and l -[35 S]cysteine) were from Amersham International (Little Chalfont, UK). .. CNBr-activated Sepharose was purchased from Pharmacia Biotech Sevrage (Uppsala, Sweden), phosphoinositol-specific phospholipase C (PiPLC), Asparaginase-N (Asp-N), and IGF-II were from Boehringer Mannheim GmbH (Mannheim, Germany), and 3,3-dithiobis(sulfosuccinimidylproprionate) (DTSSP) was from Pierce Chemical Co. (Rockford, IL). β-Glucuronidase was purified from the secretions of 13.2.1 cells (a gift of Dr. W. Sly, St. Louis University, St. Louis, MO) as described previously ( ).

    Chromatography:

    Article Title: Phosphorylation of Protease-activated Receptor-2 Differentially Regulates Desensitization and Internalization *
    Article Snippet: .. Agonist peptides TFLLRNPNDK (PAR1-specific) and SLIGKV (PAR2-specific) were synthesized as the carboxyl amide and purified by reverse-phase high-pressure chromatography (University of North Carolina Peptide Facility, Chapel Hill, NC). α-Trypsin treated with tosylamide-2-phenylethyl chloromethyl ketone, leupeptin, and cycloheximide were from Sigma. .. [32 P]Orthophosphate was purchased from PerkinElmer Life Sciences.

    Synthesized:

    Article Title: Phosphorylation of Protease-activated Receptor-2 Differentially Regulates Desensitization and Internalization *
    Article Snippet: .. Agonist peptides TFLLRNPNDK (PAR1-specific) and SLIGKV (PAR2-specific) were synthesized as the carboxyl amide and purified by reverse-phase high-pressure chromatography (University of North Carolina Peptide Facility, Chapel Hill, NC). α-Trypsin treated with tosylamide-2-phenylethyl chloromethyl ketone, leupeptin, and cycloheximide were from Sigma. .. [32 P]Orthophosphate was purchased from PerkinElmer Life Sciences.

    Infection:

    Article Title: Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control
    Article Snippet: .. For selective expression of viral immediate-early proteins, cells were incubated from 30 min prior to infection to 4 h post-infection in the presence of CHX (100 µg/ml; Sigma Aldrich), followed by incubation in the presence of actinomycin D (10 µg/ml; Sigma Aldrich) for another 12 h. Selective expression of early genes was carried out by treatment of the cells with PPA (250 µg/ml; Sigma Aldrich) for 72 h. For proteolysis inhibition experiments, cells were treated with 75 µM MG-132 (Sigma Aldrich) or 250 µM leupeptin (Sigma Aldrich) for 6 h and 24 h, respectively, before harvesting. .. Under the conditions used, MG-132 and leupeptin did not generally affect cell viability as assessed by trypan blue cell staining.

    Purification:

    Article Title: Phosphorylation of Protease-activated Receptor-2 Differentially Regulates Desensitization and Internalization *
    Article Snippet: .. Agonist peptides TFLLRNPNDK (PAR1-specific) and SLIGKV (PAR2-specific) were synthesized as the carboxyl amide and purified by reverse-phase high-pressure chromatography (University of North Carolina Peptide Facility, Chapel Hill, NC). α-Trypsin treated with tosylamide-2-phenylethyl chloromethyl ketone, leupeptin, and cycloheximide were from Sigma. .. [32 P]Orthophosphate was purchased from PerkinElmer Life Sciences.

    Concentration Assay:

    Article Title: Downregulation of p53 drives autophagy during human trophoblast differentiation
    Article Snippet: .. Leupeptin (Sigma-Aldrich, St. Louis, MO, USA) was added to cultures 24 h prior to the end of experiments at a concentration of 100 µM. ..

    Incubation:

    Article Title: Intraneuronal aggregation of the β-CTF fragment of APP (C99) induces Aβ-independent lysosomal-autophagic pathology
    Article Snippet: .. To monitor cathepsin B (CatB) activity, 60 µg of protein extracts were incubated in acetate buffer (25 mM, pH 5.5, 100 µl and 8 mM l -cysteine HCl) containing CatB substrate (CBZ-Arg-Arg-7-Amido-4-methylcoumarin, 100 µM, Sigma) in the absence or presence of leupeptin (10 µM, Sigma). .. Specific CatB activity corresponds to the leupeptin-sensitive fluorescence recorded at 320 nm (excitation) and 420 nm (emission) using a fluorescence plate reader (FLUOstar Omega, BMG Labtech, France).

    Article Title: Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control
    Article Snippet: .. For selective expression of viral immediate-early proteins, cells were incubated from 30 min prior to infection to 4 h post-infection in the presence of CHX (100 µg/ml; Sigma Aldrich), followed by incubation in the presence of actinomycin D (10 µg/ml; Sigma Aldrich) for another 12 h. Selective expression of early genes was carried out by treatment of the cells with PPA (250 µg/ml; Sigma Aldrich) for 72 h. For proteolysis inhibition experiments, cells were treated with 75 µM MG-132 (Sigma Aldrich) or 250 µM leupeptin (Sigma Aldrich) for 6 h and 24 h, respectively, before harvesting. .. Under the conditions used, MG-132 and leupeptin did not generally affect cell viability as assessed by trypan blue cell staining.

    Inhibition:

    Article Title: Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control
    Article Snippet: .. For selective expression of viral immediate-early proteins, cells were incubated from 30 min prior to infection to 4 h post-infection in the presence of CHX (100 µg/ml; Sigma Aldrich), followed by incubation in the presence of actinomycin D (10 µg/ml; Sigma Aldrich) for another 12 h. Selective expression of early genes was carried out by treatment of the cells with PPA (250 µg/ml; Sigma Aldrich) for 72 h. For proteolysis inhibition experiments, cells were treated with 75 µM MG-132 (Sigma Aldrich) or 250 µM leupeptin (Sigma Aldrich) for 6 h and 24 h, respectively, before harvesting. .. Under the conditions used, MG-132 and leupeptin did not generally affect cell viability as assessed by trypan blue cell staining.

    Activity Assay:

    Article Title: Intraneuronal aggregation of the β-CTF fragment of APP (C99) induces Aβ-independent lysosomal-autophagic pathology
    Article Snippet: .. To monitor cathepsin B (CatB) activity, 60 µg of protein extracts were incubated in acetate buffer (25 mM, pH 5.5, 100 µl and 8 mM l -cysteine HCl) containing CatB substrate (CBZ-Arg-Arg-7-Amido-4-methylcoumarin, 100 µM, Sigma) in the absence or presence of leupeptin (10 µM, Sigma). .. Specific CatB activity corresponds to the leupeptin-sensitive fluorescence recorded at 320 nm (excitation) and 420 nm (emission) using a fluorescence plate reader (FLUOstar Omega, BMG Labtech, France).

    Expressing:

    Article Title: Cytomegalovirus m154 Hinders CD48 Cell-Surface Expression and Promotes Viral Escape from Host Natural Killer Cell Control
    Article Snippet: .. For selective expression of viral immediate-early proteins, cells were incubated from 30 min prior to infection to 4 h post-infection in the presence of CHX (100 µg/ml; Sigma Aldrich), followed by incubation in the presence of actinomycin D (10 µg/ml; Sigma Aldrich) for another 12 h. Selective expression of early genes was carried out by treatment of the cells with PPA (250 µg/ml; Sigma Aldrich) for 72 h. For proteolysis inhibition experiments, cells were treated with 75 µM MG-132 (Sigma Aldrich) or 250 µM leupeptin (Sigma Aldrich) for 6 h and 24 h, respectively, before harvesting. .. Under the conditions used, MG-132 and leupeptin did not generally affect cell viability as assessed by trypan blue cell staining.

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  • 99
    Millipore lysis buffer l b
    Lysis Buffer L B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer l b/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lysis buffer l b - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    99
    Millipore lysis buffer b
    Lysis Buffer B, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer b/product/Millipore
    Average 99 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    lysis buffer b - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    85
    Millipore np 40 lysis buffer b
    Np 40 Lysis Buffer B, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/np 40 lysis buffer b/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    np 40 lysis buffer b - by Bioz Stars, 2020-05
    85/100 stars
      Buy from Supplier

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