hek293t cell lysate - untransfected cell lysate  (AMS Biotechnology)

Journal: PLoS ONE

Article Title: Serum Antibody Repertoire Profiling Using In Silico Antigen Screen

doi: 10.1371/journal.pone.0067181

Figure Lengend Snippet: ( A ). The CPA3 amino acid sequence and the 36 matching peptides with the highest match scores generated by bl2seq program are shown. The sequences of the protein matching to peptides and the corresponding peptide sequences that match to protein are underlined. ( B ). Western blot analysis of the 293T cell lysates overexpressing the recombinant CPA3 protein or transfected with empty vector. The membranes were incubated with serum from either melanoma patient (left panel) or healthy donor (right panel). The reaction was developed as described in the “Materials and Methods” section.

Article Snippet: To test if the serum antibodies of the melanoma patient will recognize the whole CPA3 protein we analyzed the 293T cells lysate (purchased from the origene.com) overexpressing the recombinant CPA3 protein using Western blotting.

Techniques: Sequencing, Generated, Western Blot, Recombinant, Transfection, Plasmid Preparation, Incubation