mouse anti lxr  (Santa Cruz Biotechnology)

Journal: Bioactive Materials

Article Title: Carrier free oral Co-delivery of atorvastatin via baicalein-copper-network for atherosclerosis therapy through senescence reversal and multi-mechanistic synergy

doi: 10.1016/j.bioactmat.2025.12.036

Figure Lengend Snippet: Effect of CMA on oxLDL uptake and foam cell formation in macrophages. (A) Optical microscopy images of RAW264.7 macrophages stained with Oil Red O (ORO) for lipid visualization. Scale bar = 50 μm. (B) Confocal laser scanning microscopy images of RAW264.7 cells incubated with DiI-oxLDL. Nuclei were counterstained with DAPI. Scale bar = 10 μm. (C) Flow cytometric analysis of the positive rate of DiI-oxLDL uptake in RAW264.7 cells. (D) Flow cytometric analysis of the mean fluorescence intensity (MFI) of DiI-oxLDL uptake in RAW264.7 cells. (E) Quantified total cholesterol (TCHO) content in foam cells derived from RAW264.7 cells. (F) Representative immunoblots showing protein expression levels of PPAR-γ, LXR-α, and ABCA-1. (G) Densitometric analysis of PPAR-γ, LXR-α, and ABCA-1 protein expression normalized to loading control, performed using ImageJ software. Data are presented as mean ± SD (n = 3–6). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 vs. Control; ns, not significant.

Article Snippet: A three-color prestained protein molecular marker (EC0020) was purchased from Shandong Sparkjade Biotechnology Co., Ltd. LXR-α antibody was purchased from MedChemExpress.

Techniques: Microscopy, Staining, Confocal Laser Scanning Microscopy, Incubation, Fluorescence, Derivative Assay, Western Blot, Expressing, Control, Software

Journal: Bioactive Materials

Article Title: Carrier free oral Co-delivery of atorvastatin via baicalein-copper-network for atherosclerosis therapy through senescence reversal and multi-mechanistic synergy

doi: 10.1016/j.bioactmat.2025.12.036

Figure Lengend Snippet: Effect of CMA on oxLDL uptake and foam cell formation in macrophages. (A) Optical microscopy images of RAW264.7 macrophages stained with Oil Red O (ORO) for lipid visualization. Scale bar = 50 μm. (B) Confocal laser scanning microscopy images of RAW264.7 cells incubated with DiI-oxLDL. Nuclei were counterstained with DAPI. Scale bar = 10 μm. (C) Flow cytometric analysis of the positive rate of DiI-oxLDL uptake in RAW264.7 cells. (D) Flow cytometric analysis of the mean fluorescence intensity (MFI) of DiI-oxLDL uptake in RAW264.7 cells. (E) Quantified total cholesterol (TCHO) content in foam cells derived from RAW264.7 cells. (F) Representative immunoblots showing protein expression levels of PPAR-γ, LXR-α, and ABCA-1. (G) Densitometric analysis of PPAR-γ, LXR-α, and ABCA-1 protein expression normalized to loading control, performed using ImageJ software. Data are presented as mean ± SD (n = 3–6). ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001 vs. Control; ns, not significant.

Article Snippet: After three 10-min washes with TBST, membranes were incubated overnight at 4 °C with primary antibodies against PPAR-γ (1:2000, Beyotime, AF7797), LXR-α (1:5000, MCE, HY- P80423 ), or ABCA-1 (1:1000, Abcam, ab307534).

Techniques: Microscopy, Staining, Confocal Laser Scanning Microscopy, Incubation, Fluorescence, Derivative Assay, Western Blot, Expressing, Control, Software