rfp ef1a puro  (AMS Biotechnology)

Journal: Biomedicines

Article Title: Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone–Iodine and Chlorhexidine in Mono and Coculture Models

doi: 10.3390/biomedicines11071855

Figure Lengend Snippet: Ki67/nuclei fluorescent area in 2D culture systems. ( A ) ADSC monocultures—the ratio significantly increased following P3 treatment compared to untreated ADSCs and decreased after P2 treatment; ( B ) NHDF monocultures—no significant ratio shifts; ( C ) A80N20 coculture—no significant ratio shifts; ( D ) A20N80 cocultures—the ratios decreased after P2 treatment compared to untreated conditions (image acquisition was performed 5 days after treatment; for each condition, a minimum of 2 images were analyzed; * p ˂ 0.0002); the results represent the overall immuno-fluorescence of Ki67 normalized to the total Hoechst area, i.e., a surrogate for total cell number; all data are displayed as means with standard errors (SEM; UT—untreated).

Article Snippet: The lentivirus contained an elongation factor 1α as promoter, a puromycin resistance gene, and either a green fluorescent protein gene (LVP426, Amsbio, Abingdon, UK) for ADSCs or a red fluorescent protein gene (LVP429, Amsbio, Abingdon, UK) for NHDFs.

Techniques: Fluorescence