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lvp001  (AMS Biotechnology)


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    AMS Biotechnology lvp001
    Lvp001, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lvp001/pm35093166-57-21-19?v=AMS+Biotechnology
    Average 96 stars, based on 14 article reviews
    lvp001 - by Bioz Stars, 2026-07
    96/100 stars

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    Long term co-culture of platinum-sensitive and resistant cells increases proliferation and sensitivity to platinum of sensitive cells. (A, B) Pictures of co-cultured PE01 and PE04 (A) and OVCAR5 WT and OVCAR5 CisR (B) ovarian cancer cells transduced with RFP or GFP lentiviral vectors. Images show cells at day 5 of co-culture at the ratios of 1:5 (sensitive to resistant) (2 replicates each, scale bar: 200 µm). (C, D) Cell proliferation (mean ± SD, n=3) measured with CCK8 assays in PE01 and PE04 (C) and OVCAR5 WT and CisR (D) cells maintained in co-culture (CC) or monoculture (MC). Cells were co-cultured for 14 days, sorted by FACS and then cultured to evaluate cell viability. Blue asterisk indicates statistical significance for the comparison between sensitive cells, marron asterisk indicates statistical significance for the comparison between resistant cells (E-H) Representative curves of cisplatin effects on cell viability of PE01 (E) , PE04 (F) , OVCAR5 WT (G) and OVCAR5 CisR (H) maintained in co-culture as described in (A, B) vs. the same cell lines in monoculture. (I, J) Comparison of cisplatin IC 50 values (means ± SD, n=3) between monocultured and co-cultured PE01 with PE04 (I) and OVCAR5 WT with CisR cells (J) . All graphs are representative of 3 independent replicates. *p<0.05; **p<0.01.

    Journal: Frontiers in Oncology

    Article Title: E2F1 mediates competition, proliferation and response to cisplatin in cohabitating resistant and sensitive ovarian cancer cells

    doi: 10.3389/fonc.2024.1304691

    Figure Lengend Snippet: Long term co-culture of platinum-sensitive and resistant cells increases proliferation and sensitivity to platinum of sensitive cells. (A, B) Pictures of co-cultured PE01 and PE04 (A) and OVCAR5 WT and OVCAR5 CisR (B) ovarian cancer cells transduced with RFP or GFP lentiviral vectors. Images show cells at day 5 of co-culture at the ratios of 1:5 (sensitive to resistant) (2 replicates each, scale bar: 200 µm). (C, D) Cell proliferation (mean ± SD, n=3) measured with CCK8 assays in PE01 and PE04 (C) and OVCAR5 WT and CisR (D) cells maintained in co-culture (CC) or monoculture (MC). Cells were co-cultured for 14 days, sorted by FACS and then cultured to evaluate cell viability. Blue asterisk indicates statistical significance for the comparison between sensitive cells, marron asterisk indicates statistical significance for the comparison between resistant cells (E-H) Representative curves of cisplatin effects on cell viability of PE01 (E) , PE04 (F) , OVCAR5 WT (G) and OVCAR5 CisR (H) maintained in co-culture as described in (A, B) vs. the same cell lines in monoculture. (I, J) Comparison of cisplatin IC 50 values (means ± SD, n=3) between monocultured and co-cultured PE01 with PE04 (I) and OVCAR5 WT with CisR cells (J) . All graphs are representative of 3 independent replicates. *p<0.05; **p<0.01.

    Article Snippet: GFP and RFP stable PE01, PE04 and OVCAR5 cells were generated by cell transduction with lentiviral vectors (Gentarget #LVP001 and #LVP023, respectively) followed by selection with puromycin and flow cytometry-based cell sorting.

    Techniques: Co-Culture Assay, Cell Culture, Transduction, Comparison