luria bertani media  (Thermo Fisher)


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    Structured Review

    Thermo Fisher luria bertani media
    Luria Bertani Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-04
    92/100 stars

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    Related Articles

    Electroporation:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH). .. Plasmid libraries were transformed into SASX38 by electroporation.

    In Vivo:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: A second Arabidopsis (Columbia) cDNA library in the Lambda UniZap vector was purchased from Stratagene (La Jolla, CA) and amplified as pBluescript plasmids by mass in vivo excision of the phage library according to the manual. .. The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH).

    Selection:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: Paragraph title: Bacterial Complementation and Selection ... The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH).

    Agarose Gel Electrophoresis:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Cycling conditions were 94°C for 2 min, followed by thirty cycles of 94°C for 15 s, 60°C for 30 s, and 72°C for 30 s, with a final elongation at 72°C for 10 min. Agarose gel electrophoresis confirmed a single PCR product for each primer set and, after product purification (ExoSAP-IT, Affymetrix, Santa Clara, CA, USA), amplicons were sequenced in both directions using the respective PCR primers (GeneWiz, South Plainfield, NJ, USA). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Clone Assay:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Additionally, a longer sequence of each of the three target genes ( > 300-bp, encompassing the respective QPCR target) was PCR-amplified (primers as in Table , PCR conditions as above) and cloned into bacterial plasmids using a TOPO TA Cloning® kit (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Mutagenesis:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: .. The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH). .. Plasmid libraries were transformed into SASX38 by electroporation.

    Article Title: Staphylococcus aureus Methicillin-Resistance Factor fmtA Is Regulated by the Global Regulator SarA
    Article Snippet: Paragraph title: Purification of SarA and SarAR90A Mutant Proteins ... Seed cultures in BL21(DE3) cells were grown overnight in Luria-Bertani media (Thermo-Fisher).

    Picogreen Assay:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above). .. Plasmid DNA was then linearized with EcoR1 restriction endonuclease (Invitrogen, see above; ESM Figure ) and quantified using a PicoGreen® assay.

    TA Cloning:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Additionally, a longer sequence of each of the three target genes ( > 300-bp, encompassing the respective QPCR target) was PCR-amplified (primers as in Table , PCR conditions as above) and cloned into bacterial plasmids using a TOPO TA Cloning® kit (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Microscopy:

    Article Title: Borrelia burgdorferi Surface-Localized Proteins Expressed during Persistent Murine Infection Are Conserved among Diverse Borrelia spp.
    Article Snippet: Cells were enumerated under dark-field microscopy using a Petroff-Hausser counting chamber. pH shifts were performed as previously described ( ). .. Escherichia coli was maintained in Luria-Bertani media (Fisher, Pittsburgh, PA) supplemented when needed with either 100 μg/ml ampicillin (Fisher) or 40 μg/ml kanamycin (Invitrogen, Carlsbad, CA).

    Purification:

    Article Title: Staphylococcus aureus Methicillin-Resistance Factor fmtA Is Regulated by the Global Regulator SarA
    Article Snippet: Paragraph title: Purification of SarA and SarAR90A Mutant Proteins ... Seed cultures in BL21(DE3) cells were grown overnight in Luria-Bertani media (Thermo-Fisher).

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above). .. Inserts were sequenced in both directions using M13R and M13(-21)F primers (Genewiz, South Plainfield, NJ, USA) to confirm the presence and correct orientation of the respective inserts.

    Real-time Polymerase Chain Reaction:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Additionally, a longer sequence of each of the three target genes ( > 300-bp, encompassing the respective QPCR target) was PCR-amplified (primers as in Table , PCR conditions as above) and cloned into bacterial plasmids using a TOPO TA Cloning® kit (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Sequencing:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Additionally, a longer sequence of each of the three target genes ( > 300-bp, encompassing the respective QPCR target) was PCR-amplified (primers as in Table , PCR conditions as above) and cloned into bacterial plasmids using a TOPO TA Cloning® kit (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Amplification:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: A second Arabidopsis (Columbia) cDNA library in the Lambda UniZap vector was purchased from Stratagene (La Jolla, CA) and amplified as pBluescript plasmids by mass in vivo excision of the phage library according to the manual. .. The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH).

    cDNA Library Assay:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: A second Arabidopsis (Columbia) cDNA library in the Lambda UniZap vector was purchased from Stratagene (La Jolla, CA) and amplified as pBluescript plasmids by mass in vivo excision of the phage library according to the manual. .. The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH).

    Construct:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above). .. Linearized plasmids (encoding the respective QPCR target sequences) were used to construct a seven-level log10 -dilution standard curve (107 –101 copies per reaction, in triplicate) prior to each set of QPCR reactions.

    Expressing:

    Article Title: Staphylococcus aureus Methicillin-Resistance Factor fmtA Is Regulated by the Global Regulator SarA
    Article Snippet: Seed cultures in BL21(DE3) cells were grown overnight in Luria-Bertani media (Thermo-Fisher). .. SarA expression was initiated by adding 1 mM isopropyl-1-thio-β-D-galactopyranoside (IPTG; Rose Scientific, Edmonton, Canada).

    Polymerase Chain Reaction:

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Additionally, a longer sequence of each of the three target genes ( > 300-bp, encompassing the respective QPCR target) was PCR-amplified (primers as in Table , PCR conditions as above) and cloned into bacterial plasmids using a TOPO TA Cloning® kit (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    Transformation Assay:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH). .. Plasmid libraries were transformed into SASX38 by electroporation.

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: Plasmids were then transformed into One Shot® Mach1TM T1 phage-resistant chemically competent Escherichia coli (Life Technologies, see above). .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above).

    other:

    Article Title: Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity
    Article Snippet: Bacterial strains and growth conditions E. coli NovaBlue (Novagen, Madison, WI) and ER2566 (Novagen) strains were routinely grown in Luria-Bertani media (LB, Invitrogen, Carlsbad, CA).

    Plasmid Preparation:

    Article Title: Development of Protoporphyrinogen Oxidase as an Efficient Selection Marker for Agrobacterium tumefaciens-Mediated Transformation of Maize
    Article Snippet: A second Arabidopsis (Columbia) cDNA library in the Lambda UniZap vector was purchased from Stratagene (La Jolla, CA) and amplified as pBluescript plasmids by mass in vivo excision of the phage library according to the manual. .. The Escherichia coli hemG mutant SASX38 ( ) was kindly provided by Dr. Alex Sasarman (Department of Microbiology and Immunology, Universite de Montreal, Quebec, Canada) and maintained on Luria-Bertani media containing 20 μg mL–1 hematin (United States Biochemicals, USB Corporation, Cleveland OH).

    Article Title: Partitioning of Respiration in an Animal-Algal Symbiosis: Implications for Different Aerobic Capacity between Symbiodinium spp.
    Article Snippet: .. After 24 h bacterial growth in Luria-Bertani media at 37°C, plasmids were extracted and purified using a PureLink® Plasmid MiniPrep kit (Invitrogen, see above). .. Inserts were sequenced in both directions using M13R and M13(-21)F primers (Genewiz, South Plainfield, NJ, USA) to confirm the presence and correct orientation of the respective inserts.

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  • News
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  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
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  • 92
    Thermo Fisher luria bertani media
    Luria Bertani Media, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani media/product/Thermo Fisher
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    luria bertani media - by Bioz Stars, 2020-04
    92/100 stars
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