Structured Review

Merck & Co luria bertani media
Luria Bertani Media, supplied by Merck & Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani media/product/Merck & Co
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
luria bertani media - by Bioz Stars, 2020-04
93/100 stars

Images

Related Articles

Centrifugation:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. After centrifugation 20,000 × g for 60 min, the supernatant fraction was loaded onto a Q-Sepharose column (Amersham Pharmacia Biotech) and then eluted fractions containing Ch-CPN were applied to a t-Butyl HIC column (Bio-Rad) .

Over Expression:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: .. The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. After centrifugation 20,000 × g for 60 min, the supernatant fraction was loaded onto a Q-Sepharose column (Amersham Pharmacia Biotech) and then eluted fractions containing Ch-CPN were applied to a t-Butyl HIC column (Bio-Rad) .

Hydrophobic Interaction Chromatography:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. After centrifugation 20,000 × g for 60 min, the supernatant fraction was loaded onto a Q-Sepharose column (Amersham Pharmacia Biotech) and then eluted fractions containing Ch-CPN were applied to a t-Butyl HIC column (Bio-Rad) .

Purification:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. The purified recombinant Ch-CPN protein was concentrated to ~ 17 mg ml−1 for crystallization .

Expressing:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: Crystallography The full-length gene of Ch-CPN WT coding for a 521-residue polypeptide was inserted downstream of the tac promoter of the expression plasmid pTAC24b + (courtesy of Dr. Zeev Pancer) using Nde I and Xho I (NEB Inc.) . .. The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) .

Transformation Assay:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: .. The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. After centrifugation 20,000 × g for 60 min, the supernatant fraction was loaded onto a Q-Sepharose column (Amersham Pharmacia Biotech) and then eluted fractions containing Ch-CPN were applied to a t-Butyl HIC column (Bio-Rad) .

Recombinant:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. The purified recombinant Ch-CPN protein was concentrated to ~ 17 mg ml−1 for crystallization .

Crystallization Assay:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) . .. The purified recombinant Ch-CPN protein was concentrated to ~ 17 mg ml−1 for crystallization .

Plasmid Preparation:

Article Title: Structural and mechanistic characterization of an archaeal-like chaperonin from a thermophilic bacterium
Article Snippet: The plasmid DNA was transformed into the E. coli BL21 (DE3) (Novagen). .. The transformed cells were grown to an OD600 of 0.5 at 37 °C in Luria-Bertani media (Merck) with 50 mg ml−1 kanamycin (Duchefa) and the overexpression of Ch-CPN was induced by the addition of 1 mM isopropyl-1-thio-β-D-galactopyranoside (Duchefa) .

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 88
    Merck & Co culture media luria bertani lb
    Culture Media Luria Bertani Lb, supplied by Merck & Co, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/culture media luria bertani lb/product/Merck & Co
    Average 88 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    culture media luria bertani lb - by Bioz Stars, 2020-04
    88/100 stars
      Buy from Supplier

    Image Search Results