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Difco luria bertani media
Luria Bertani Media, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani media/product/Difco
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
luria bertani media - by Bioz Stars, 2020-04
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water
Article Snippet: Bacterial strains and culture condition used for spiking water Gram-positive Bacillus amyloliquefaciens (strain BS6), acid-fast Mycobacterium smegmatis and two gram-negative strains, Escherichia coli and Yersinia enterocolitica , obtained from the Microbiome Laboratory (Department of Animal Science, University of Manitoba, Canada) were cultured in the liquid Luria-Bertani media (LB; Difco, Fisher Scientific, Edmonton, AB, Canada) on a rotary shaker (New Brunswick Scientific, Edison, NJ, USA). .. To experimentally create mini-microbial communities, ultra-pure HPLC-grade (Fisher Scientific, Ottawa, ON, Canada) was used as the water source and spiked with mixtures of bacterial species present at a concentration of 1.5 × 104 CFU/ml in triplicate as follow: (1) four live bacterial species, (2) four dead bacterial species, (3) dead gram-positive and acid-fast species plus live gram-negative species, (4) dead gram-negative species plus live gram-positive and acid-fast species, and (5) HPLC grade water without any bacterial culture (negative control).

Clone Assay:

Article Title: Common Cell Shape Evolution of Two Nasopharyngeal Pathogens
Article Snippet: .. For cloning experiments, E . coli DH5α was grown at 37°C in Luria-Bertani Media (Difco). .. When required, antibiotics were added as follows: chloramphenicol (30 μg/ml for E . coli ; 5 μg/ml for Neisseria sp.), kanamycin (50 μg/ml for E . coli ; 100 μg/ml for Neisseria sp.) and erythromycin (300 μg/ml for E . coli ; 3 μg/ml for Neisseria sp.) and sub-inhibiting concentration of penicillin G (0.12 μg/ml).

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C. .. For antibiotic selection, chloramphenicol (10 μg mL−1 ), kanamycin (50 μg mL−1 ), spectinomycin (100 μg mL−1 ), and ampicillin (100 μg mL−1 ) were added to the medium.

Negative Control:

Article Title: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water
Article Snippet: Bacterial strains and culture condition used for spiking water Gram-positive Bacillus amyloliquefaciens (strain BS6), acid-fast Mycobacterium smegmatis and two gram-negative strains, Escherichia coli and Yersinia enterocolitica , obtained from the Microbiome Laboratory (Department of Animal Science, University of Manitoba, Canada) were cultured in the liquid Luria-Bertani media (LB; Difco, Fisher Scientific, Edmonton, AB, Canada) on a rotary shaker (New Brunswick Scientific, Edison, NJ, USA). .. To experimentally create mini-microbial communities, ultra-pure HPLC-grade (Fisher Scientific, Ottawa, ON, Canada) was used as the water source and spiked with mixtures of bacterial species present at a concentration of 1.5 × 104 CFU/ml in triplicate as follow: (1) four live bacterial species, (2) four dead bacterial species, (3) dead gram-positive and acid-fast species plus live gram-negative species, (4) dead gram-negative species plus live gram-positive and acid-fast species, and (5) HPLC grade water without any bacterial culture (negative control).

Selection:

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C. .. With E. faecium TX82, gentamicin (Sigma Chemical Co., St. Louis, MO) was used at 125 µg/mL (150 µg/mL for selection of transformants) and chloramphenicol at 10 µg/mL and, with E. coli , gentamicin was used at 25 µg/mL.

Article Title: Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains
Article Snippet: E. coli strains were grown in Luria-Bertani media (Difco Laboratories, Detroit, Mich.). .. The following antibiotic concentrations were used with enterococci: chloramphenicol, 10 μg/ml; erythromycin, 15 μg/ml (for selection of transformants); erythromycin, 200 μg/ml [for selection of naturally resistant E. faecium strains due to msrC ( ) plus erm (B)]; gentamicin, 125 μg/ml; and tetracycline, 10 μg/ml.

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C. .. With E. faecium TX82, gentamicin (Sigma Chemical Co., St. Louis, MO) was used at 125 μg/mL (150 μg/mL for selection of transformants) and chloramphenicol at 10 μg/mL and, with E. coli , gentamicin was used at 25 μg/mL.

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: For antibiotic selection, erythromycin (5 μg mL−1 ), chloramphenicol (10 μg mL−1 ), kanamycin (200 μg mL−1 ), and spectinomycin (100 μg mL−1 ) were added to the medium. .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C.

Mutagenesis:

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: The MGAS6143Δscl1 -, MGAS6183Δscl1 -, and MGAS10870Δscl1 -inactivated mutant strains have been previously described (Han et al., ; Caswell et al., ; Flores et al., ). .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C.

Isolation:

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: MGAS315 was isolated from a patient with streptococcal toxic syndrome in the 1980's (Musser et al., ) and MGAS10870 was isolated from a patient with soft tissue infection in Ontario in 2002 (Beres et al., ). .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C.

Construct:

Article Title: Importance of Two Enterococcus faecium Loci Encoding Gls-like Proteins for In Vitro Bile Salts Stress Response and Virulence
Article Snippet: Plasmid constructs were given pTEX numbers, and their host strains were assigned respective TX numbers. .. E. coli strains were grown routinely in Luria-Bertani media (Difco Laboratories) and enterococci in either brain heart infusion (BHI) or Todd-Hewitt (TH) broth or agar (Difco Laboratories) at 37°C.

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: Plasmid constructs were given pTEX numbers and their host strains corresponding TX numbers. .. E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C.

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: Plasmid constructs were given pTEX numbers and their host strains corresponding TX numbers. .. E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C.

Article Title: Importance of the Collagen Adhesin Ace in Pathogenesis and Protection against Enterococcus faecalis Experimental Endocarditis
Article Snippet: All constructs were given TX numbers and plasmids from these constructs were assigned respective pTEX numbers ( ). .. E. coli strains were grown in Luria-Bertani media (Difco Laboratories, Detroit, Mich.).

Concentration Assay:

Article Title: Genetic Analysis of the Role of the Transfer Gene, traN, of the F and R100-1 Plasmids in Mating Pair Stabilization during Conjugation
Article Snippet: Cells were grown in Luria-Bertani media (LB; 1% Difco tryptone, 0.5% Difco yeast extract, 1% NaCl [BDH Inc.]) at 37°C on a tube roller or shaker. .. Glucose was added to cultures to a final concentration of 100 mM to repress expression from an isopropyl-β- d -thiogalactopyranoside (IPTG)-inducible promoter or as a general growth enhancer.

Article Title: Common Cell Shape Evolution of Two Nasopharyngeal Pathogens
Article Snippet: For cloning experiments, E . coli DH5α was grown at 37°C in Luria-Bertani Media (Difco). .. When required, antibiotics were added as follows: chloramphenicol (30 μg/ml for E . coli ; 5 μg/ml for Neisseria sp.), kanamycin (50 μg/ml for E . coli ; 100 μg/ml for Neisseria sp.) and erythromycin (300 μg/ml for E . coli ; 3 μg/ml for Neisseria sp.) and sub-inhibiting concentration of penicillin G (0.12 μg/ml).

Article Title: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water
Article Snippet: Bacterial strains and culture condition used for spiking water Gram-positive Bacillus amyloliquefaciens (strain BS6), acid-fast Mycobacterium smegmatis and two gram-negative strains, Escherichia coli and Yersinia enterocolitica , obtained from the Microbiome Laboratory (Department of Animal Science, University of Manitoba, Canada) were cultured in the liquid Luria-Bertani media (LB; Difco, Fisher Scientific, Edmonton, AB, Canada) on a rotary shaker (New Brunswick Scientific, Edison, NJ, USA). .. To experimentally create mini-microbial communities, ultra-pure HPLC-grade (Fisher Scientific, Ottawa, ON, Canada) was used as the water source and spiked with mixtures of bacterial species present at a concentration of 1.5 × 104 CFU/ml in triplicate as follow: (1) four live bacterial species, (2) four dead bacterial species, (3) dead gram-positive and acid-fast species plus live gram-negative species, (4) dead gram-negative species plus live gram-positive and acid-fast species, and (5) HPLC grade water without any bacterial culture (negative control).

Incubation:

Article Title: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water
Article Snippet: Bacterial strains and culture condition used for spiking water Gram-positive Bacillus amyloliquefaciens (strain BS6), acid-fast Mycobacterium smegmatis and two gram-negative strains, Escherichia coli and Yersinia enterocolitica , obtained from the Microbiome Laboratory (Department of Animal Science, University of Manitoba, Canada) were cultured in the liquid Luria-Bertani media (LB; Difco, Fisher Scientific, Edmonton, AB, Canada) on a rotary shaker (New Brunswick Scientific, Edison, NJ, USA). .. For each of the cultured species, half of the culture was killed by placing flasks in a water bath at 95 °C for 30 min and confirming that aliquots (100 μl) did not produce any visible colonies on the LB agar (Difco) upon incubation.

other:

Article Title: High-throughput metal susceptibility testing of microbial biofilms
Article Snippet: Bacteria were grown in Luria-Bertani media (pH 7.1, Difco) enriched with 0.001% vitamin B1 (LB + B1) or in the minimal salts vitamins glucose (MSVG, pH 7.1) of Teitzel and Parsek [ ].

Cell Culture:

Article Title: Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance
Article Snippet: Pph strains (Table ) were cultured at 25° C for 48 h on King’s B (KB) agar plates (Difco, UK). .. Overnight cultures were grown in Luria–Bertani media (LB, Difco, UK) at 25° C. Antibiotics and supercoiling inhibitors were used at the following concentrations (μg/ml): kanamycin (Km) 50, novobiocin (1–100) and ciprofloxacin (0.5–5).

Article Title: Comparison of DNA-, PMA-, and RNA-based 16S rRNA Illumina sequencing for detection of live bacteria in water
Article Snippet: .. Bacterial strains and culture condition used for spiking water Gram-positive Bacillus amyloliquefaciens (strain BS6), acid-fast Mycobacterium smegmatis and two gram-negative strains, Escherichia coli and Yersinia enterocolitica , obtained from the Microbiome Laboratory (Department of Animal Science, University of Manitoba, Canada) were cultured in the liquid Luria-Bertani media (LB; Difco, Fisher Scientific, Edmonton, AB, Canada) on a rotary shaker (New Brunswick Scientific, Edison, NJ, USA). .. E. coli and Y. enterocolitica were cultured at 200 rpm and 37 °C for 16–18 h. M. smegmatis was cultured at 200 rpm and 37 °C for 72 h. B. amyloliquefaciens was cultured at 180 rpm and 30 °C for 16 to 18 h. All bacteria were collected from late log cultures to minimize the number of dead bacteria within the cultures.

Article Title: A low frequency persistent reservoir of a genomic island in a pathogen population ensures island survival and improves pathogen fitness in a susceptible host
Article Snippet: Bacterial and plant growth conditions Pph 1302A::NCR (Pitman et al ., ) and RJ3 (Jackson et al ., ) were cultured at 25°C for 48 h on Kings B (KB) agar plates (Difco, UK). .. Overnight cultures were grown in Luria–Bertani media (Difco) at 25°C shaking at 200 rpm.

Expressing:

Article Title: Genetic Analysis of the Role of the Transfer Gene, traN, of the F and R100-1 Plasmids in Mating Pair Stabilization during Conjugation
Article Snippet: Cells were grown in Luria-Bertani media (LB; 1% Difco tryptone, 0.5% Difco yeast extract, 1% NaCl [BDH Inc.]) at 37°C on a tube roller or shaker. .. Glucose was added to cultures to a final concentration of 100 mM to repress expression from an isopropyl-β- d -thiogalactopyranoside (IPTG)-inducible promoter or as a general growth enhancer.

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C. .. For antibiotic selection, chloramphenicol (10 μg mL−1 ), kanamycin (50 μg mL−1 ), spectinomycin (100 μg mL−1 ), and ampicillin (100 μg mL−1 ) were added to the medium.

Infection:

Article Title: Unique Footprint in the scl1.3 Locus Affects Adhesion and Biofilm Formation of the Invasive M3-Type Group A Streptococcus
Article Snippet: MGAS315 was isolated from a patient with streptococcal toxic syndrome in the 1980's (Musser et al., ) and MGAS10870 was isolated from a patient with soft tissue infection in Ontario in 2002 (Beres et al., ). .. Cloning experiments were performed in XL-1 Blue and TB1 E. coli cells, while protein expression experiments were performed in BL21 and TB1 E. coli cells grown in Luria-Bertani media (Difco Laboratories) at 37°C.

Plasmid Preparation:

Article Title: Importance of Two Enterococcus faecium Loci Encoding Gls-like Proteins for In Vitro Bile Salts Stress Response and Virulence
Article Snippet: Plasmid constructs were given pTEX numbers, and their host strains were assigned respective TX numbers. .. E. coli strains were grown routinely in Luria-Bertani media (Difco Laboratories) and enterococci in either brain heart infusion (BHI) or Todd-Hewitt (TH) broth or agar (Difco Laboratories) at 37°C.

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: Plasmid constructs were given pTEX numbers and their host strains corresponding TX numbers. .. E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C.

Article Title: Characterization of the ebpfm pilus-encoding operon of Enterococcus faecium and its role in biofilm formation and virulence in a murine model of urinary tract infection
Article Snippet: Plasmid constructs were given pTEX numbers and their host strains corresponding TX numbers. .. E. faecium strains were routinely grown in/on brain heart infusion (BHI) (Difco Laboratories, Detroit, MI) broth/agar or, for some experiments (see below), in tryptic soy broth supplemented with 0.25% glucose (TSBG) or on trypticase soy agar supplemented with 5% sheep blood (Becton Dickinson, Franklin Lakes, NJ), and E. coli in Luria-Bertani media (Difco Laboratories) at 37°C.

Hybridization:

Article Title: Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains
Article Snippet: Enterococcal isolates initially identified to the species level by biochemical tests were confirmed by colony hybridization ( ) using an intragenic ace probe for E. faecalis ( , ) and an aac (6′) -Ii probe for E. faecium ( ). .. E. coli strains were grown in Luria-Bertani media (Difco Laboratories, Detroit, Mich.).

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  • 97
    Difco luria bertani lb broth
    Luria Bertani Lb Broth, supplied by Difco, used in various techniques. Bioz Stars score: 97/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb broth/product/Difco
    Average 97 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb broth - by Bioz Stars, 2020-04
    97/100 stars
      Buy from Supplier

    94
    Difco luria bertani lb media
    Luria Bertani Lb Media, supplied by Difco, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luria bertani lb media/product/Difco
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    luria bertani lb media - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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