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Difco luria bertani media
Luria Bertani Media, supplied by Difco, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/luria bertani media/product/Difco
Average 91 stars, based on 7 article reviews
Price from $9.99 to $1999.99
luria bertani media - by Bioz Stars, 2020-01
91/100 stars

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Clone Assay:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Paragraph title: Cloning and expression of rChIL-26 ... Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Amplification:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Cloning and expression of rChIL-26 To clone full-length ChIL-26, the predicted ChIL-26 coding sequence (GenBank accession # XM_004937561) was amplified from total RNA of the intestinal mucosal layer using the following restriction enzyme-anchored primers: Eco RI-anchored forward primer, 5′- CGGAATTC ATGAAAAATGTTTTCAGTCATCTTGG-3′; and Hin dIII-anchored reverse primer, 5′- CCAAGCTT TACTATGGTTTGGATGTAGGCCT-3′ (restriction sites are underlined). .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Agarose Gel Electrophoresis:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C. .. The digested ChIL-26 fragment was purified from an agarose gel using a QIAQuick gel extraction kit (QIAgen, Valencia, CA, USA) and then ligated into digested pET32a (EMD Millipore, Billerica, MA, USA).

Synthesized:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: The newly synthesized full-length ChIL-26 gene was directly inserted into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA), followed by transformation into Escherichia coli TOP10 (Invitrogen). .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Isolation:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Total RNA was isolated using TRizol reagent (Invitrogen, Carlsbad, CA, USA) as described [ ] from the intestinal mucosal layer of White Leghorn chickens, kindly provided by the Animal Biosciences and Biotechnology Laboratory (Beltsville, MD, USA) of the USDA-Agricultural Research Service. .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Polymerase Chain Reaction:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Polymerase chain reaction (PCR) was performed to amplify the full-length ChIL-26 cDNA, under the following conditions: initial denaturation at 94 °C for 3 min, 35 cycles of denaturation at 94 °C for 30 s, annealing at 60 °C for 30 s, and extension at 72 °C for 30 s and a final extension at 72 °C for 5 min. .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Cell Culture:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C. .. A transformant was selected by a combination of PCR screening and sequencing (Genotech Inc., Daejeon, Republic of Korea).

Purification:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C. .. The digested ChIL-26 fragment was purified from an agarose gel using a QIAQuick gel extraction kit (QIAgen, Valencia, CA, USA) and then ligated into digested pET32a (EMD Millipore, Billerica, MA, USA).

Concentration Assay:

Article Title: Heme Concentration Dependence and Metalloporphyrin Inhibition of the System I and II Cytochrome c Assembly Pathways ▿ Assembly Pathways ▿ †
Article Snippet: All E. coli strains used in this work were grown aerobically (with shaking at 300 rpm) at 37°C in Luria-Bertani media (Difco). .. Aminolevulinic acid (ALA) (Sigma) was used at a concentration of 50 μg ml−1 (300 μM), unless indicated otherwise.

Selection:

Article Title: Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains
Article Snippet: E. coli strains were grown in Luria-Bertani media (Difco Laboratories, Detroit, Mich.). .. The following antibiotic concentrations were used with enterococci: chloramphenicol, 10 μg/ml; erythromycin, 15 μg/ml (for selection of transformants); erythromycin, 200 μg/ml [for selection of naturally resistant E. faecium strains due to msrC ( ) plus erm (B)]; gentamicin, 125 μg/ml; and tetracycline, 10 μg/ml.

Construct:

Article Title: Importance of Two Enterococcus faecium Loci Encoding Gls-like Proteins for In Vitro Bile Salts Stress Response and Virulence
Article Snippet: Plasmid constructs were given pTEX numbers, and their host strains were assigned respective TX numbers. .. E. coli strains were grown routinely in Luria-Bertani media (Difco Laboratories) and enterococci in either brain heart infusion (BHI) or Todd-Hewitt (TH) broth or agar (Difco Laboratories) at 37°C.

Expressing:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Paragraph title: Cloning and expression of rChIL-26 ... Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Sequencing:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Cloning and expression of rChIL-26 To clone full-length ChIL-26, the predicted ChIL-26 coding sequence (GenBank accession # XM_004937561) was amplified from total RNA of the intestinal mucosal layer using the following restriction enzyme-anchored primers: Eco RI-anchored forward primer, 5′- CGGAATTC ATGAAAAATGTTTTCAGTCATCTTGG-3′; and Hin dIII-anchored reverse primer, 5′- CCAAGCTT TACTATGGTTTGGATGTAGGCCT-3′ (restriction sites are underlined). .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Gel Extraction:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C. .. The digested ChIL-26 fragment was purified from an agarose gel using a QIAQuick gel extraction kit (QIAgen, Valencia, CA, USA) and then ligated into digested pET32a (EMD Millipore, Billerica, MA, USA).

Transformation Assay:

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C. .. A transformant was selected by a combination of PCR screening and sequencing (Genotech Inc., Daejeon, Republic of Korea).

Plasmid Preparation:

Article Title: Importance of Two Enterococcus faecium Loci Encoding Gls-like Proteins for In Vitro Bile Salts Stress Response and Virulence
Article Snippet: Plasmid constructs were given pTEX numbers, and their host strains were assigned respective TX numbers. .. E. coli strains were grown routinely in Luria-Bertani media (Difco Laboratories) and enterococci in either brain heart infusion (BHI) or Todd-Hewitt (TH) broth or agar (Difco Laboratories) at 37°C.

Article Title: The novel chicken interleukin 26 protein is overexpressed in T cells and induces proinflammatory cytokines
Article Snippet: The newly synthesized full-length ChIL-26 gene was directly inserted into the pCR2.1-TOPO vector (Invitrogen, Carlsbad, CA, USA), followed by transformation into Escherichia coli TOP10 (Invitrogen). .. Transformed E. coli TOP10 cells were cultured overnight in Luria–Bertani media (Difco™ and BBL™, NJ, USA) at 37 °C.

Hybridization:

Article Title: Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains
Article Snippet: Enterococcal isolates initially identified to the species level by biochemical tests were confirmed by colony hybridization ( ) using an intragenic ace probe for E. faecalis ( , ) and an aac (6′) -Ii probe for E. faecium ( ). .. E. coli strains were grown in Luria-Bertani media (Difco Laboratories, Detroit, Mich.).

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